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Effect of aging on the response of biochemical markers in the rabbit subjected to short-term partial bladder obstruction

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Abstract

Purpose Partial bladder outlet obstruction (PBOO) results in marked biochemical alterations in the bladder. In this study, we focused on comparison of thapsigargin sensitive sarco/endoplasmic reticulum Ca2+ ATPase activity (SERCA) and Citrate Synthase after short term PBOO in young versus old rabbits. Materials and methods A total of 20 young and 20 mature male rabbits were divided into 4 sub-groups of 5 rabbits each (4 obstructed and 1 sham-control rabbit). The rabbits in the groups were evaluated after 1, 3, 7, and 14 days of obstruction, respectively. The activities of SERCA and citrate synthase were examined as markers for sarcoplasmic reticular calcium storage and release and mitochondrial function, respectively. Results The SERCA activity of bladder body smooth muscle in the young animals increased at 7 and 14 days. For the old rabbits, the SERCA activity decreased significantly by 1 day and remained this level throughout the course of obstruction, and was significantly lower than young at all time periods. The citrate synthase activity in the young animals decreased over the 1–7 days, and then returned toward control level by 14 days following obstruction. In the old animals, citrate synthase activity of bladder body smooth muscle progressively decreased over the course of the study, and was significantly lower in the old than the young animals after 14 days obstructed. Conclusion The urinary bladders of the young rabbits have a considerable greater ability to adapt to PBOO than do those of the old rabbits. The deterioration of mitochondrial and SR function may be important mechanisms underlying geriatric voiding dysfunction.

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Acknowledgments

This material is based upon work supported in part by the Office of Research and Development Medical Research Service, Department of Veteran’s Affairs and in part by NIH grant RO-1-DK 067114 and the Capital Region Medical Research Foundation

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Authors and Affiliations

  1. Department of Pediatric Surgery, Gulhane Military Medical Faculty, Ankara, Turkey

    Ahmet Guven

  2. Albany College of Pharmacy, 106 New Scotland Ave, Albany, NY, 12208, USA

    Ahmet Guven, Wei-Yu Lin, Robert E. Leggett, Robert M. Levin & Anita Mannikarottu

  3. Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Chia-Yi, Taiwan, ROC

    Wei-Yu Lin

  4. Albany Medical College, Albany, NY, USA

    Barry A. Kogan, Robert M. Levin & Anita Mannikarottu

  5. Stratton VA Medical Center, Albany, NY, USA

    Robert M. Levin

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  1. Ahmet Guven
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  2. Wei-Yu Lin
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  3. Robert E. Leggett
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  4. Barry A. Kogan
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  5. Robert M. Levin
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  6. Anita Mannikarottu
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Correspondence to Robert M. Levin.

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Guven, A., Lin, WY., Leggett, R.E. et al. Effect of aging on the response of biochemical markers in the rabbit subjected to short-term partial bladder obstruction. Mol Cell Biochem 306, 213–219 (2007). https://doi.org/10.1007/s11010-007-9571-x

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  • DOI: https://doi.org/10.1007/s11010-007-9571-x

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