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Nitricoxide synthase-induced oxidative stress in prolonged alcoholic myopathies of rats

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Abstract

Previous studies showed that nitricoxide synthase (NOS) and oxidative stress can induce skeletal muscle atrophy in the muscular dystrophy and inclusion-body myopathy. There is a correlation between NOS and oxidative stress. However, it is not clear, whether there are some changes of the NOS activity in prolonged alcoholic myopathy (PAM), and whether NOS activity has relation to amyotrophy of PAM. We established experimental alcoholic myopathy model of rats by prolonged alcohol intake. We found that there is a reduction in GSH-px (P < 0.05) and an increase of SOD (P < 0.05), MDA (P < 0.05) and iNOS (P < 0.05) in the plantaris of the experimental group by spectrophotometer. In the soleus of the experimental group, except for MDA showed an increase (P < 0.05), the other enzymes showed no obvious difference (P > 0.05). The immunohistochemistry results showed that there was obvious expression of iNOS in the cytoplasm of plantaris in the experimental group and there was no expression of iNOS in the control group. There was a decrease of nNOS expression on the membranes of the plantaris cells in the experimental group by immunofluorescence. Meanwhile, we found the expression of nNOS in some cytoplasm. Our results suggested that NOS might be an important factor during the development of PAM. We could infer that there are some disturbances with regard to output and scavenging of free radical in PAM. Alcohol can induce the oxidative stress reaction and further result in imbalance of the oxidant-antioxidant status in the organism.

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Abbreviations

PAM:

Prolonged alcoholic myopathy

NOS:

Nitricoxide synthase

iNOS:

Inducible nitric oxide synthase

nNOS:

Neuronal nitric oxide synthase

SOD:

Superoxide dismutase

MDA:

Maleic dialdehyde

GSH-px:

Glutathione peroxidase

PBS:

Phosphate buffered saline

References

  1. Preedy VR, Ohlendieck K, Adachi J et al (2003) The importance of alcohol—induced muscle disease. J Muscle Res Cell Motil 24(1):55–63

    Article  PubMed  CAS  Google Scholar 

  2. Lang CH, Frost RA, Summer AD et al (2005) Molecular mechanisms responsible for alcohol-induced myopathy in skeletal muscle and heart. Int J Biochem Cell Biol 10:2180–2195

    Article  CAS  Google Scholar 

  3. Preedy VR, Adachi J, Peters TJ et al (2001) Recent advances in the pathology of alcoholic myopathy. Alcohol Clin Exp Res 2:54–59

    Google Scholar 

  4. Preedy VR, Patel VB, Reilly ME et al (1999) Oxidants, antioxidants and alcohol: implications for skeletal and cardiac muscle. Front Biosci 4:58–66

    Article  Google Scholar 

  5. Adachi J, Asano M, Ueno Y et al (2000) 7a-and7b-hydroperoxycholest-5-en-3b-ol in muscle as indices of oxidative stress: response to ethanol dosage in rats. Alcohol Clin Exp Res 24(5):675–681

    PubMed  CAS  Google Scholar 

  6. Fernandez-Sola J, Garcia G, Elena M, Tobias E et al (2002) Muscle antioxidant status in prolonged alcoholism. Res Soc Alcohol 26:1858–1862

    Article  CAS  Google Scholar 

  7. Preedy VR, Adachi J, Asano M et al (2002) Free radicals in alcoholic myopathy: indices of damage and preventive studies. Free Radic Biol Med 32:683–687

    Article  PubMed  CAS  Google Scholar 

  8. Rando TA (2002) Oxidative stress and the pathogenesis of muscular dystrophies. Lippincott Williams Wilkins 81:175–186

    Google Scholar 

  9. Mukherjee M, Mittal B (2004) Muscular dystrophies. Indian J Pediatr 71(2):161–168

    PubMed  Google Scholar 

  10. Yang CC, Alvarez RB, Engel WK et al (1998) Nitric oxide—induced oxidative stress in autosomal recessive and dominant inclusion-body myopathies. Brain 121:1089–1097

    Article  PubMed  Google Scholar 

  11. Preedy VR, Peters TJ (1988) The effect of prolonged ethanol ingestion on protein metabolism in type-I- and type-II-fibre-rich skeletal muscles of the rat. Biochem J 254:631–639

    PubMed  CAS  Google Scholar 

  12. Preedy VR, McIntosh A, Bonner AB et al (1996) Ethanol dosage regimens in studies of ethanol toxicity: influence of nutrition and surgical interventions. Addict Biol 1:255–262

    Article  PubMed  CAS  Google Scholar 

  13. Lieber CS, Decarli LM (1989) Liquid diet technique of ethanol administration. Alcohol 24(3):197–211

    CAS  Google Scholar 

  14. Lieber CS, DeCarli LM (1994) Animal models of prolonged ethanol toxicity. Methods Enzymol 233:585–594

    Article  PubMed  CAS  Google Scholar 

  15. Wang JF, Cheng XY, Chu HY et al (2006) Establishment of the model of prolonged alcoholic myopathy in rat. Chin J Neuromed 5:382–385

    Google Scholar 

  16. Stamler JS, Meissner G (2001) Physiology of nitric oxide synthade in skeletal muscle. Physiol Rev 81:209–237

    PubMed  CAS  Google Scholar 

  17. Koch OR, Pani G, Borrello S et al (2004) Oxidative stress and antioxidant defenses in ethanol-induced cell injury. Mol Aspects Med 25:191–198

    Article  PubMed  CAS  Google Scholar 

  18. Zimiani K, Guarnier A, Miranda HC et al (2005) Nitric oxide mediated oxidative stress injury in rat skeletal muscle subjected to ischemia/reperfusion as evaluated by chemiluminescence. Nitric Oxide 13:196–203

    Article  PubMed  CAS  Google Scholar 

  19. Vassilakopoulos T, Deckman G, Kebbewar M et al (2003) Regulation of nitric oxide production in limb and ventilatory muscles during prolonged exercise training. Physiol Lung Cell Mol Physiol 284:452–457

    Google Scholar 

  20. Bando TA (2001) Role of nitric oxide in the pathogenesis of muscular dystrophies: a “two hit” hypothesis of the cause of muscle necrosis. Microsc Res Tech 55:223–235

    Article  Google Scholar 

  21. Reid MB (2001) Nitric oxide, reactive oxygen species, and skeletal muscle contraction. Lippincott Williams Wilkins 33:371–376

    CAS  Google Scholar 

  22. Tidball JG (2005) Inflammatory processes in muscle injury and repair. Physiol Regul Integr Comp Physiol 288:345–353

    Google Scholar 

  23. Lawler JM, Song W (2002) Specificity of antioxidant enzyme inhibition in skeletal muscle to reactive nitrogen species donors. Biochem Biophys Res Commun 294(5):1093–1100

    Article  PubMed  CAS  Google Scholar 

  24. Altura BM, Altura BT, Gebrewold A (1990) Comparative effects of ethanol acetaldehyde and acetate on arterioles and venules in skeletal muscle: direct in sity studies on the microcirculation and their possible relationship to alcoholic myopathy. Microcirc Endothelium Lymphatics 6:107–126

    PubMed  CAS  Google Scholar 

  25. Hobbs AJ, Fukuto JM, Ignarro LJ (1994) Formation of free nitric oxide from l-arginine by nitric oxide synthase: direct enhancement of generation by superoxide dismutase. Proc Natl Acad Sci USA 23:10992–10996

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from National Ministry of Personnel Foundation of China (No. 129,2005).

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Authors and Affiliations

  1. Department of Neurology, Dalian Centre Hospital, Dalian, 116033, Liaoning Province, China

    Jianfeng Wang, Hua Zhao, Xueying Cheng & Yanli Liu

  2. Department of Histology and Embryology, Dalian Medical University, 465 Zhongshan Road, Dalian, 116027, Liaoning Province, China

    Haiying Chu, Wei Jin, Jinyao Zhao, Bo Liu, Yanfang Ding & Haiying Ma

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  1. Jianfeng Wang
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  2. Haiying Chu
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  3. Hua Zhao
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  7. Jinyao Zhao
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  9. Yanfang Ding
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Correspondence to Haiying Chu.

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Haiying Chu is the co-first author.

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Wang, J., Chu, H., Zhao, H. et al. Nitricoxide synthase-induced oxidative stress in prolonged alcoholic myopathies of rats. Mol Cell Biochem 304, 135–142 (2007). https://doi.org/10.1007/s11010-007-9494-6

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  • DOI: https://doi.org/10.1007/s11010-007-9494-6

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