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Effect of aging on the expression of intracellular Ca2+ transport proteins in a rat heart

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Abstract

Aging process is accompanied by various biological dysfunctions including altered calcium homeostasis. Modified calcium handling might be responsible for changed cardiac function and potential development of the pathological state. In the present study we compared the mRNA and protein levels of the intracellular Ca2+-handling proteins—inositol 1,4,5-trisphosphate receptor (IP3R), ryanodine receptor (RyR), sarcoplasmic reticulum Ca2+ pump (SERCA2), and also transient receptor potential C (TRPC) channels in cardiac tissues of 5-, 15-, and 26-month-old rats. Aging was accompanied by significant increase in the mRNA levels of IP3R and TRPC channels in both ventricles and atria, but mRNA level of the type 2 RyR was unchanged. Protein content of the IP3R1 correlated with mRNA levels, in the left ventricle of 15- and 26-month-old rats the value was approximately 1.8 and 2.8-times higher compared to 5-month-old rats. No significant differences were observed in mRNA and protein levels of the SERCA2 among 5-month-old and aged rats. However, Ca2+-ATPase activity significantly decreased with age, activities in 5-, 15-, and 26-month-old rats were 421.2 ± 13.7, 335.5 ± 18.1 and 304.6 ± 14.8 nmol Pi min-1 mg−1. These results suggest that altered transporting activity and/or gene expression of Ca2+-handling proteins of intracellular Ca2+ stores might affect cardiac function during aging.

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Acknowledgment

This work was supported by VEGA 1/2263/05, 2/6078, 2/5125, APVT–51-027404, and Genomika SP 51/0280800/0280802.

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Authors and Affiliations

  1. Department of Biochemistry, Jessenius Faculty of Medicine, Comenius University, Mala Hora 4, Martin, Slovak Republic

    P. Kaplan, E. Babusikova, P. Racay, J. Lehotsky, A. Drgova & D. Dobrota

  2. Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34, Bratislava, Slovak Republic

    D. Jurkovicova, S. Hudecova, M. Sirova & O. Krizanova

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  1. P. Kaplan
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  2. D. Jurkovicova
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  3. E. Babusikova
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  10. O. Krizanova
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Correspondence to O. Krizanova.

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Kaplan, P., Jurkovicova, D., Babusikova, E. et al. Effect of aging on the expression of intracellular Ca2+ transport proteins in a rat heart. Mol Cell Biochem 301, 219–226 (2007). https://doi.org/10.1007/s11010-007-9414-9

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  • DOI: https://doi.org/10.1007/s11010-007-9414-9

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