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Functional characterization of AATF transcriptome in human leukemic cells

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Abstract

The study, addressed to explore the transcriptional expression and regulation of Apoptosis-antagonizing transcription factor (AATF) gene within various types of human leukemic cell lines, revealed that AATF gene was overexpressed ubiquitously in all the leukemic cell lines studied and this upregulation was accompanied by c-myc gene overamplification in these cells. Downregulation of AATF gene transcription within leukemic cells not only resulted in the downregulation of c-myc gene and vice-versa but also contributed to apoptosis leading to cell death. Further, the link between AATF expression and leukemic cellular apoptosis involved PI3K/Akt pathway. Based on these results we propose that AATF gene may be of crucial importance in maintaining the leukemic state of a cell compartment through its ability to initiate cell proliferation coupled with repression of cellular apoptosis.

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References

  1. Kaul D Receptor Ck and leukemogenesis. Leukemia Res, 22: 389–394, 1998

    Article  CAS  Google Scholar 

  2. Burgdorf S, Leister P, Scheidtmann KH TSG101 interacts with apoptosis antagonizing transcription factor and enhances androgen receptor-mediated transcription by promoting its monoubiquitination. J Biol Chem 279: 17524–17534, 2004

    Article  PubMed  CAS  Google Scholar 

  3. Bruno T, De Angelis R, De Nicola F, Barbato C, Di Padova M, Corbi N, Libri V, Benassi B, Mattei E, Chersi A, Soddu S, Floridi A, Passananti C, Fanciulli M Che-1 affects cell growth by interacting with the recruitment of HDAC-1 by Rb Cancer cell, 2: 387–399, 2002

    Article  PubMed  CAS  Google Scholar 

  4. Page G, Lodige I, Kogel D, Scheidtmann KH AATF, a novel transcription factor that interacts with DLK/ZIP kinase FEBS Lett, 462: 187–191, 1999

    Article  PubMed  CAS  Google Scholar 

  5. Di Padova M, Bruno T, De Nicola F, Iezzi S, D’Angelo C, Gallo R, Nicosia D, Corbi N, Biroccio A, Floridi A, Passananti C, Fanciulli M Che-1 arrests human carcinoma cell proliferation by displacing HDAC-1 from the p21WAF1/C1P1 promoter J Biol Chem, 278: 36496–36504, 2003

    Article  PubMed  CAS  Google Scholar 

  6. Moller MB Molecular control of the cell cycle in cancer: biological and clinical aspects Dan Med Bull, 50(2): 118–138, 2003

    PubMed  CAS  Google Scholar 

  7. Preuss U, Landsberg G, Scheidtmann KH Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase Nucleic Acids Res, 31(3): 878–885, 2003

    Article  PubMed  CAS  Google Scholar 

  8. Palmieri C, Coombes RC, Vigushin DM Targeted histone deacetylase inhibition for cancer prevention and therapy Prog Drug Res: 63, 147–181, 2005

    Article  PubMed  CAS  Google Scholar 

  9. Downward J Mechanisms and consequences of activation of protein kinase B/ Akt Curr Opin Cell Biol, 10: 262–267, 1998

    Article  PubMed  CAS  Google Scholar 

  10. Kaul D, Rani R, Sehgal R Receptor-Ck regulates giardia encystations process Mol Cell Biochem, 225: 167–169, 2001

    Article  PubMed  CAS  Google Scholar 

  11. Anand PK, Kaul D, Sharma M Green tea polyphenol inhibits Mycobacterium tuberculosis survival within human macrophages Int J Biochem Cell Biol, 38: 600–609, 2006

    Article  PubMed  CAS  Google Scholar 

  12. Sikand K, Kaul D, Varma N Receptor Ck-dependent signaling regulates hTERT gene transcription BMC Cell Biol, 7: 2–22, 2006

    Article  PubMed  CAS  Google Scholar 

  13. Kaul D, Anand PK Regulation of PPAR-γ gene in human promyelocytic HL-60 cell line Leukemia Res, 27: 683–686, 2003

    Article  CAS  Google Scholar 

  14. Albert T, Wells J, Funk JO, Pullner A, Raschke EE, Stelzer G, Meisterernst M, Farnham PJ, Eick D The chromatin structure of the dual c-myc promoter p1/p2 is regulated by separate elements J Biol Chem, 276: 20482–20490, 2001

    Article  PubMed  CAS  Google Scholar 

  15. Evan GI, Vousden KH Proliferation, cell cycle and apoptosis in cancer Nature, 411: 342–348, 2001

    Article  PubMed  CAS  Google Scholar 

  16. Kasibhatla S, Tseng B Why target apoptosis in cancer treatment. Mol Cancer Ther, 2: 573–580, 2003

    PubMed  CAS  Google Scholar 

  17. Cheema KS, Mishra SK, Rangnekar VM, Tari AM, Kumar R, Lopez-Berestein G Par-4 transcriptionally regulates BCL-2 through a WT1-binding site on the bcl-2 promoter J Biol Chem, 278(22): 19995–20005, 2003

    Article  PubMed  CAS  Google Scholar 

  18. Goswami A, Burikhanov R, de Thonel A, Fujita N, Goswami M, Zhao Y, Eriksson JE, Tsuruo T, Rangnekar VM Binding and phosphorylation of par-4 by Akt is essential for cancer cell survival Mol Cell 20(1): 33–44, 2005

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Molecular Biology Unit,Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India

    Deepak Kaul & Aanchal Mehrotra

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  1. Deepak Kaul
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  2. Aanchal Mehrotra
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Correspondence to Deepak Kaul.

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Kaul, D., Mehrotra, A. Functional characterization of AATF transcriptome in human leukemic cells. Mol Cell Biochem 297, 215–220 (2007). https://doi.org/10.1007/s11010-006-9317-1

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  • DOI: https://doi.org/10.1007/s11010-006-9317-1

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