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Characterization of a novel low-molecular-mass dual specificity phosphatase-4 (LDP-4) expressed in brain

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Abstract

Dual-specificity phosphatases (DSPs), which dephosphorylate proteins at Ser/Thr as well as Tyr residues, are thought to be involved in critical signaling events such as control of MAP kinases (MAPKs). We have isolated the cDNA for a novel DSP and termed it low molecular mass DSP-4 (LDP-4). LDP-4 is composed of 211 amino acids with a predicted molecular mass of 23.9-kDa. Northern blot analysis using various mouse tissues showed that the LDP-4 transcript was expressed exclusively in brain. In situ hybridization showed that brain expression of LDP-4 was ubiquitous except for the hippocampus. When expressed in COS-7 cells, FLAG-tagged LDP-4 protein was present within the nucleus and Golgi apparatus. LDP-4 expression did not reduce phosphorylation levels of MAPKs, but rather evoked activation of JNK and p38.

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Acknowledgements

We thank Dr. J. M. Kryiakis for pMT-HA-p38α and Dr.␣M.␣Karin for pSRα-HA-JNK1 and pSRα-HA-ERK2. We are grateful to Mrs. E. Yoshida for secretarial assistance. This work was supported in part by HIROMI Medical Research Foundation, Osaka Cancer Research Foundation, grants-in-aid for Scientific Research (B) provided by the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Division of Biochemical Oncology and Immunology, Institute for Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, Japan

    Kentaro Takagaki, Nobuhiro Tanuma, Takeshi Satoh & Kunimi Kikuchi

  2. Division of Cancer Chemotherapy, Miyagi Cancer Center Research Institute, 47-1 Nodayama, Medeshima-Shiode, Natori, Miyagi, Japan

    Hiroshi Shima & Miyuki Nomura

  3. Department of Anatomy, Hokkaido University School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan

    Masahiko Watanabe

Authors
  1. Kentaro Takagaki
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  2. Hiroshi Shima
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  3. Nobuhiro Tanuma
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  4. Miyuki Nomura
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  5. Takeshi Satoh
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  6. Masahiko Watanabe
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  7. Kunimi Kikuchi
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Correspondence to Hiroshi Shima.

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Takagaki, K., Shima, H., Tanuma, N. et al. Characterization of a novel low-molecular-mass dual specificity phosphatase-4 (LDP-4) expressed in brain. Mol Cell Biochem 296, 177–184 (2007). https://doi.org/10.1007/s11010-006-9313-5

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  • DOI: https://doi.org/10.1007/s11010-006-9313-5

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