Abstract
We have studied the effect of exogenous administration of hydrogen peroxide (H2O2) on phagocytic activity of human neutrophils. The treatment of cells with increasing concentrations of H2O2 evoke a significant elevation of phagocytic function assayed as phagocytic index, percentage and efficiency; and was similar to that induced by the calcium mobilising agonist formyl-methionyl-leucyl-phenylalanine (fMLP). This stimulatory effect was reduced by pre-treatment of neutrophils with catalase and abolished in neutrophils loaded with the intracellular calcium quelator dimethyl BAPTA. In the absence of extracellular calcium, treatment of cells with H2O2 resulted in a increase in [Ca2+] i , indicating the release of calcium from intracellular stores. H2O2 abolished the typical calcium release stimulated by the physiological agonist fMLP, while depletion of agonist-sensitive calcium pools by fMLP was able to prevent H2O2-induced calcium release. We conclude that H2O2 induces calcium release from agonist-sensitive stores and consequently increase the phagocytosis process.
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Acknowledgments
This work was supported by Ministerio de Educación y Ciencia Grant (BFU 2004-00165). The authors would like to thank Mrs. Elena Circujano Vadillo for her excellent technical support.
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Bejarano, I., Terrón, M.P., Paredes, S.D. et al. Hydrogen peroxide increases the phagocytic function of human neutrophils by calcium mobilisation. Mol Cell Biochem 296, 77–84 (2007). https://doi.org/10.1007/s11010-006-9301-9
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DOI: https://doi.org/10.1007/s11010-006-9301-9