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Human Homologue of Seta Binding Protein 1 Interacts with Cathepsin B and Participates in TNF-Induced Apoptosis in Ovarian Cancer Cells

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Abstract

Lysosomal cysteine protease cathepsin B has been reported to play an important role in apoptosis of many different cancer cells, but the regulation of cathepsin B in apoptosis is poorly understood. Human homologue of SETA binding protein 1 (hSB1) was identified to interact with cathepsin B by yeast-two hybrid method, and the interaction was confirmed in vitro GST pull-down assay and in vivo coimmunoprecipitation experiment. hSB1 was co-localized with cathepsin B in cellular lysosomes. Our previous study has shown that TNF can induce ovarian cancer cells OV-90 apoptosis and the apoptosis process is cathepsin B-depended. Here we provide evidence that overexpression of cathepsin B-interacting protein hSB1 could suppress TNF-triggered apoptosis in OV-90 cells, but has no effect on cellular cathepsin B activity. hSB1 may function as a regulator of cathepsin B-mediated apoptosis.

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, 200433, PR China

    Jian-Ping Liu, Nan-Song Liu, Han-Ying Yuan, Qian Guo, Hong Lu & Yu-Yang Li

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  1. Jian-Ping Liu
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  2. Nan-Song Liu
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  3. Han-Ying Yuan
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  4. Qian Guo
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  5. Hong Lu
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  6. Yu-Yang Li
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Correspondence to Hong Lu.

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Liu, JP., Liu, NS., Yuan, HY. et al. Human Homologue of Seta Binding Protein 1 Interacts with Cathepsin B and Participates in TNF-Induced Apoptosis in Ovarian Cancer Cells. Mol Cell Biochem 292, 189–195 (2006). https://doi.org/10.1007/s11010-006-9214-7

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