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The expression of CD40-CD40L and activities of matrix metalloproteinases in atherosclerotic rats

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Abstract

This study investigated the expression of CD40, CD40 ligand (CD40L) and matrix metalloproteinases (MMPs) in dietary-induced atherosclerosis in rats. Wister rats were fed with high cholesterol diet (As group, n = 6) or with normal diet (N group, n = 6). Blood cells that express CD40 and CD40L were sorted by flow cytometry, the MMP-2 and MMP-9 were measured by zymography method. The morphological locations of MMP-2 and MMP-9 in the aorta were studied with immunohistochemistry and by microscopy. The results showed that the expression of CD40, CD40L and matrix metalloproteinase were higher in As group than those in control group. The MMP-2 and MMP-9 were positive in As group but negative in control group by immunohistochemistry study. Our results suggest that the expression of CD40 and CD40L in the blood cells and the activities of MMP-2 and MMP-9 in plasma were higher in As group than those in Normal group, indicating that they may contribute to the formation of atherosclerosis.

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Abbreviations

CD40L:

CD40 ligand

DAB:

diaminobenzidine

FACS:

fluorescence-activated cell sorter

FITC:

fluorescence isothiocyanate

IL:

interleukin

MCP-1:

monocyte chemoattractant protein-1

MMP:

matrix metalloproteinase

PBS:

phosphate-buffered saline

TF:

tissue factor

TNF:

tumor necrosis factor

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Authors and Affiliations

  1. Department of Cardiology, First Affiliated Hospital of the Medical College, Shantou University, Shantou, 515041, Guangdong Province, People's Republic of China

    Min Wu & Yu-Guang Li

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  1. Min Wu
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  2. Yu-Guang Li
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Correspondence to Yu-Guang Li.

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Wu, M., Li, YG. The expression of CD40-CD40L and activities of matrix metalloproteinases in atherosclerotic rats. Mol Cell Biochem 282, 141–146 (2006). https://doi.org/10.1007/s11010-006-1741-8

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  • DOI: https://doi.org/10.1007/s11010-006-1741-8

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