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Decreased keratinocyte motility in skin wound on mice lacking the epidermal fatty acid binding protein gene

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Abstract

Fatty acids are shown to be important in various skin functions. Fatty acid binding protein (FABP) is postulated to serve as a lipid shuttle, solubilizing hydrophobic fatty acids and delivering them to the appropriate metabolic system. Among the FABP family proteins, epidermal-type FABP (E-FABP) is solely expressed in keratinocyte but its specific role in skin is not yet fully established. We found an elevated expression of E-FABP in regenerative keratinocytes of healing wounds. However, E-FABP null mice showed no marked differences compared to wild type mice in the process of wound closure, in vivo. On the other hand, in keratinocyte culture, E-FABP gene disruption decreased the cell motility, but did not affect the cell proliferation. E-FABP deletion may be compensated for in vivo by the microenvironment comprised of various cells such as fibroblasts and endothelial cells around the wound. Our analyses suggest that the E-FABP elevation may be necessary for the activation of cell motility within regenerative epidermis during wound healing.

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Authors and Affiliations

  1. Department of Dermatology, Tohoku University Graduate School of Medicine, Tohoku, Japan

    Yoshiyuki Kusakari, Eisaku Ogawa, Hiroshi Watanabe, Michiyo Kimura, Hachiro Tagami, Setsuya Aiba & Ryuhei Okuyama

  2. Department of Histology, Tohoku University Graduate School of Medicine, Tohoku, Japan

    Yuji Owada, Noriko Kitanaka & Hisatake Kondo

  3. Department of Dermatology, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai, 980-8574, Japan

    Ryuhei Okuyama

Authors
  1. Yoshiyuki Kusakari
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  2. Eisaku Ogawa
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  3. Yuji Owada
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  4. Noriko Kitanaka
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  5. Hiroshi Watanabe
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  6. Michiyo Kimura
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  7. Hachiro Tagami
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  8. Hisatake Kondo
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  9. Setsuya Aiba
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  10. Ryuhei Okuyama
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Correspondence to Ryuhei Okuyama.

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Kusakari, Y., Ogawa, E., Owada, Y. et al. Decreased keratinocyte motility in skin wound on mice lacking the epidermal fatty acid binding protein gene. Mol Cell Biochem 284, 183–188 (2006). https://doi.org/10.1007/s11010-005-9048-8

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  • DOI: https://doi.org/10.1007/s11010-005-9048-8

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