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Immunomodulatory effects of seabuckthorn (Hippophae rhamnoides L.) against chromium (VI) induced immunosuppression

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Abstract

The present study reports the immunomodulatory effects of seabuckthorn (Hippophae rhamnoides L.) leaf extract on cellular and humoral immune response by studying delayed-type hypersensitivity response, IL-2, IL-4 and γ-IFN levels and antibody titres in chromium-induced immunosuppressed animals. Oral feeding of chromium (30 mg/kg bw) significantly inhibited antibody production and S-RBC induced delayed-type hypersensitivity response. Administration of leaf extract (100 mg/kg bw) along with chromium significantly inhibited chromium-induced immunosuppression. To understand the immunomodulatory mechanism of leaf extract, in vitro studies were carried out using rat lymphocytes. Addition of chromium resulted in a significant decrease in lymphocyte size and increased ROS generation. The leaf extract of seabuckthorn significantly inhibited chromium-induced reactive oxygen species (ROS) generation and maintained the cell size identical to that of control cells. Chromium treatment markedly inhibited the mitochondrial transmembrane potential by larger lymphocytes in particular, while the leaf extract restored the same significantly. Chromium also inhibited significantly concanavalin A (ConA) induced IL-2, IL-4 and γ-IFN production in rat lymphocytes. The leaf extract (100 μg/ml) alone stimulated IL-2 and γ-IFN production even in the absence of ConA and also inhibited chromium-induced decline in IL-2 and γ-IFN production but it did not change IL-4 production. These observations suggest that the leaf extract of seabuckthorn has significant immunomodulatory activity and specifically activates the cell-mediated immune response. (Mol Cell Biochem 278: 101–109, 2005)

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References

  1. Dale MM, Foreman JC, Fan TD: Textbook of Immunopharmacology, 3rd edn. Blackwell Scientific Publication, London

  2. Patwardhan B, Kalbag D, Patki PS, Nagsampagi BA: Search of immunomodulatory agents – a review. Indian Drugs 28: 249–254, 1991

    Google Scholar 

  3. Charak Samhita. Translator: Shree Gulabkunverba Ayurvedic Society, Jamnagar, India, 1949

  4. Burger AM, Mengs U, Schuler JB, Fiebig HH: Anticancer activity of an aqueous mistletoe extract (AME) in syngeneic murine tumor models. Anticancer Res 21: 1965–1968, 2001

    PubMed  Google Scholar 

  5. Gracious ross R, Selvasubramanian S, Jayasundar S: Immunomodulatory activity of Punica granatum in rabbits – a preliminary study. J Ethnopharmacol 78: 85–87, 2001

    Article  PubMed  Google Scholar 

  6. Zhao W, Ye Q, Tan X, Jiang H, Li X, Chen K, Kinghorn AD: Three new sesquiterpene glycosides from Dendrobium nobile with immunomodulatory activity. J Nat Prod 64: 1196–2000, 2001

    Article  PubMed  Google Scholar 

  7. Kubena KS, Mcmurray DN: Nutrition and the immune system: A review of the nutrient-nutrient interactions. J Am Diet Assoc 96: 1156–1164, 1996

    Article  PubMed  Google Scholar 

  8. Bagchi D, Bagchi M, Stohs SJ: Chromium (VI) induced oxidative stress, apoptotic cell death and modulation of p53 tumor suppressor gene. Mol Cell Biochem 222: 149–158, 2002

    Article  Google Scholar 

  9. Shrivastava R, Upreti R, Seth P, Chaturvedi U: Effects of chromium on the immune system. FEMS Immunol Med Microbiol 34: 1–7, 2002

    Article  PubMed  Google Scholar 

  10. Tanigawa T, Araki T, Minato N: Decrease in Leu-11a negative lymphocytes in relation to natural killer cell activity in chromate workers. Br J Internal Med 48: 211–213, 1991

    Google Scholar 

  11. Cohen MD, Zeikoff JT, Chen LC, Schlesinger RB: Immunotoxicologic effect of inhaled chromium: Role of particle solubility and coexposure to ozone. Toxicol Appl Pharmacol 152: 30–40, 1998

    Article  PubMed  Google Scholar 

  12. Rousi A: The genus Hippophae L., A taxonomic study. Ann Bot Fenn 8: 177–227, 1971

    Google Scholar 

  13. Eccleston C, Baoru Y, Tahvonen R, Kallio H, Rimbach GH, Minihane AM: Effects of an antioxidant rich juice (Seabuckthorn) on risk factors for coronary heart disease in humans. J Nutr Biochem 13: 346–354, 2002

    Article  PubMed  Google Scholar 

  14. Yang B, Kalimo KO, Tahvonen, RL, Mattila LM, Katjisto JK, Kallio HP: Effects of dietary supplementation with sea buckthorn (Hippophae rhamnoides) seed and pulp oils on atopic dermatitis. J Nutr Biochem 10: 622–630, 1999

    Article  PubMed  Google Scholar 

  15. Suleyman H, Gumustekin K, Taysi S, Keles S, Oztasan N, Aksas O, Altinkaynak K, Timur H, Akcay F, Akar S, Dane S, Gul M: Beneficial effects of Hippophae rhamnoides L. on nicotine induced oxidative stress in rat blood compared with vitamin E. Biol Pharm Bull 25: 1133–1136, 2002

    Article  PubMed  Google Scholar 

  16. Goel HC, Prasad J, Singh S, Sagar RK, Kumar IP, Sinha AK: Radioprotection by a herbal preparation of Hippophae rhamnoides, RH-3 against whole body lethal irradiation in mice. Phytomed 9: 15–25, 2002

    Article  Google Scholar 

  17. Fushion Z: Comparative research on the contents of seabuckthorn oil and its physical chemical properties, composition of fatty acids and biologically active substance in Northwestern China. Chinese oil and Fat (shanxi) 2: 25–30, 1987

    Google Scholar 

  18. Chen Y, Jiang Z, Qin W: Chemistry composition and characteristics of Seabuckthorn fruit and its oil. Chem Ind Forest Products (in Chinese) 10: 163–175, 1999

    Google Scholar 

  19. Yao Y, Tigerstedt P: Variation of vitamin C concentration between and within natural seabuckthorn (Hippophae rhamnoides L.) populations. Acta Agric Scand 42: 12–17, 1992

    Google Scholar 

  20. Yang B, Kallio HP: Fatty acid composition of lipids in Seabuckthorn (Hippophae rhamnoides L.) berries of different origins. J Agric Food Chem 49: 1939–1947, 2001

    Article  PubMed  Google Scholar 

  21. Geetha S, Sai Ram M, Singh V, Ilavazhagan G, Sawhney RC: Anti-oxidant and immunomodulatory properties of Seabuckthorn (Hippophae rhamnoides) an in vitro study. J Ethnopharmacol 79: 373–378, 2002

    Article  PubMed  Google Scholar 

  22. Sugiyama M, Tsuzuki K, Ogura R: Effect of ascorbic acid on DNA damage, cytotoxicity, glutathione reductase and formation of paramagnetic chromium in Chinese Hamster V-79 cells treated with sodium chromate (VI). J Biol Chem 266: 3383–3386, 1991

    PubMed  Google Scholar 

  23. Sai Ram M, Sharma SK, Ilavazhagan G, Kumar D, Selvamurthy W: Immunomodulatory properties of NIM-76, a volatile fraction from neem oil. J Ethnopharmacol 55: 133–139, 1997

    Article  PubMed  Google Scholar 

  24. Atal CK, Sharma ML, Kaul Khajuria A: Immunomodulatory agents of plant origin. Preliminary screening. J Ethnopharmacol 41: 185–192, 1986

    Google Scholar 

  25. Meydani SN, Wu D, Santos MS, Hayek MG: Antioxidants and immune status in aged persons: Overview of present evidence. Am J Clin Nutr 62: 1462S–1476S, 1995

    PubMed  Google Scholar 

  26. Sugiyama M: Role of physiological antioxidants in chromium (VI)-induced cellular injury. Free Radic Biol Med 12: 397–407, 1992

    Article  PubMed  Google Scholar 

  27. Shi X, Chiu A, Chen CT, Halliwell B, Castranova V, Vallyathan V: Reduction of chromium (VI) and its relationship to carcinogenesis. J Toxicol Environ Health B Crit Rev 2: 87–100, 1999

    Article  PubMed  Google Scholar 

  28. Kumar IP, Namita S, Goel HC: Modulation of chromatin organization by RH-3, a preparation of Hippophae rhamnoides, a possible role in radioprotection. Mol Cell Biochem 238: 1–9, 2002

    Article  PubMed  Google Scholar 

  29. Novruzov EN: Flavonoids of different forms of Seabuckthorn (Hippophae rhamnoides L.) growing in Azerbaijan. In: V Singh and PK Khosla (eds). Proceedings of International Workshop on Seabuckthorn, New Delhi, India, 2001, pp 140–146.

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Authors and Affiliations

  1. Defence Institute of Physiology & Allied Sciences, Lucknow Road, Timarpur, Delhi, 110054, India

    S. Geetha, Vandana Singh, M. Sai Ram, G. Ilavazhagan, P. K. Banerjee & R. C. Sawhney

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  1. S. Geetha
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  2. Vandana Singh
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  3. M. Sai Ram
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  4. G. Ilavazhagan
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  5. P. K. Banerjee
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  6. R. C. Sawhney
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Correspondence to R. C. Sawhney.

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Geetha, S., Singh, V., Ram, M.S. et al. Immunomodulatory effects of seabuckthorn (Hippophae rhamnoides L.) against chromium (VI) induced immunosuppression. Mol Cell Biochem 278, 101–109 (2005). https://doi.org/10.1007/s11010-005-7095-9

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  • DOI: https://doi.org/10.1007/s11010-005-7095-9

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