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Construction of a vector system for molecular cloning in Bacillus subtilis and Escherichia coli

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REFERENCES

  1. Schallmey M., Singh A., Ward O.P. 2004. Developments in the use of Bacillus species for industrial production. Can. J. Microbiol. 50, 1–17.

    Article  CAS  PubMed  Google Scholar 

  2. Hardy F., Vriend G., van der Vinne B., et al. 1994. The effect of engineering surface loops on the thermal stability of Bacillus subtilis neutral protease. Protein Eng. 7, 425–430.

    CAS  PubMed  Google Scholar 

  3. Leifert C., Li H., Chidburee S., et al. 1995. Antibiotic production and biocontrol activity by Bacillus subtilis CL27 and Bacillus pumilus CL45. J. Appl. Bacteriol. 78, 97–108.

    CAS  PubMed  Google Scholar 

  4. Hofemeister J., Conrad B., Adler B., et al. 2004. Genetic analysis of the biosynthesis of non-ribosomal peptide-and polyketide-like antibiotics, iron uptake and biofilm formation by Bacillus subtilis A1/3. Mol. Genet. Genomics Online, 1617–4615

  5. Kunst F., Ogasawara N., Moszer I., et al. 1997. The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature. 390, 249–256.

    CAS  PubMed  Google Scholar 

  6. Janniere L., Gruss A., Ehrlich S.D. 1993. Plasmids. In: Bacillus subtilis and Other Gram Positive Bacteria: Biochemistry, Physiology, and Molecular Genetics. Eds. Sonenshein A.L., Hoch J.A., Losick R. Washington, D.C.: Am. Soc. Microbiol., 625–644.

    Google Scholar 

  7. Perez-Arellano I., Zuniga M., Perez-Martinez G. 2001. Construction of compatible wide-host-range shuttle vectors for lactic acid bacteria and Escherichia coli. Plasmid. 46, 106–116.

    CAS  PubMed  Google Scholar 

  8. Anagnostopoulos C., Spizizen J. 1961. Requirements for transformation in Bacillus subtilis. J. Bacteriol. 81, 741–746.

    CAS  Google Scholar 

  9. Bullock W.O., Fernandes J.M., Short J.M. 1987. Bio-Techniques. 5, 376–379.

    CAS  Google Scholar 

  10. Chambers S.P., Prior S.E., Barstow D.A., et al. 1988. The pMTL nic-cloning vectors: 1. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing. Gene. 68, 139–149.

    CAS  PubMed  Google Scholar 

  11. Sambrook J., Fritsch E.F., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press.

    Google Scholar 

  12. Birnboim H.C., Doly J. 1979. A rapid alkaline exaction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7, 1513–1523.

    CAS  PubMed  Google Scholar 

  13. Bron S. 1990. Plasmids. In: Molecular Biological Methods for Bacillus. Eds. Harwood C.R., Cutting S.M. Chichester: Wiley, 75–174.

    Google Scholar 

  14. Titok M.A., Chapuis J., Selezneva Y.V., et al. 2003. Bacillus subtilis soil isolates: Plasmid replicon analysis and construction of a new theta-replicating vector. Plasmid. 49, 53–62.

    CAS  PubMed  Google Scholar 

  15. Janniere L., Bruand C., Ehrlich S.D. 1990. Structurally stable Bacillus subtilis cloning vectors. Gene. 87, 53–61.

    CAS  PubMed  Google Scholar 

  16. Joseph P., Fantino J.-R., Herbaud M.-L., et al. 2001. Rapid orientated cloning in a shuttle vector allowing modulated gene in Bacillus subtilis. FEMS Microbiol. Lett. 205, 91–97.

    CAS  PubMed  Google Scholar 

  17. Bron S., Luxen E. 1985. Segregational instability of pUB110-derived recombinant plasmids in Bacillus subtilis. Plasmid. 14, 235–244.

    CAS  PubMed  Google Scholar 

  18. Dabert P., Ehrlich S.D., Gruss A. 1992. High-molecular-weight linear multimer formation by single-stranded DNA plasmids in Escherichia coli. J. Bacteriol. 174, 173–178.

    CAS  PubMed  Google Scholar 

  19. Janniere L., Ehrlich S.D. 1987. Recombination between short repeated sequences is more frequent in plasmids than in the chromosome of Bacillus subtilis. Mol. Gen. Genet. 210, 116–121.

    CAS  PubMed  Google Scholar 

  20. Dabert P., Ehrlich S.D., Gruss A. 1992. Chi sequence protects against RecBCD degradation of DNA in vivo. Proc. Natl. Acad. Sci. USA. 89, 12073–12077.

    CAS  PubMed  Google Scholar 

  21. Gruss A., Ehrlich S.D. 1988. Insertion of foreign DNA into plasmids from Gram-positive bacteria induces formation of high-molecular-weight plasmid multimers. J. Bacteriol. 170, 1183–1190.

    CAS  PubMed  Google Scholar 

  22. De la Hoz A.B., Ayora S., Sitkiewicz I., et al. 2000. Plasmid copy-number control and better-than-random segregation genes of pSM19035 share a common regulator. Proc. Natl. Acad. Sci. USA. 97, 728–733.

    PubMed  Google Scholar 

  23. Lereclus D., Arantes O. 1992. spbA locus ensures the segregational stability of pTH1030, a novel type of Gram-positive replicon. Mol. Microbiol. 6, 35–46.

    CAS  PubMed  Google Scholar 

  24. Swinfield T.J., Janniere L., Ehrlich S.D., et al. 1991. Characterization of a region of the Enterococcus faecalis plasmid pAM beta 1 which enhances the segregational stability of pAM beta 1-derived cloning vectors in Bacillus subtilis. Plasmid. 26, 209–221.

    CAS  PubMed  Google Scholar 

  25. Renault P., Corthier G., Goupil N., et al. 1996. Plasmid vectors for Gram-positive bacteria switching from high to low copy number. Gene. 183, 175–182.

    CAS  PubMed  Google Scholar 

  26. Poyart C., Trieu-Cuot P. 1997. A broad-host-range mobilizable shuttle vector for the construction of transcriptional fusions to β-galactosidase in Gram-positive bacteria. FEMS Microbiol. Lett. 156, 193–198.

    CAS  PubMed  Google Scholar 

  27. Arantes O., Lereclus D. 1991. Construction of cloning vectors for Bacillus thuringiensis. Gene. 108, 115–119.

    CAS  PubMed  Google Scholar 

  28. Itaya M., Tanaka T. 1997. Experimental surgery to create subgenomes of Bacillus subtilis 168. Proc. Natl. Acad. Sci. USA. 94, 5378–5382.

    CAS  PubMed  Google Scholar 

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Translated from Molekulyarnaya Biologiya, Vol. 39, No. 2, 2005, pp. 345–348.

Original Russian Text Copyright © 2005 by Lagodich, Cherva, Shtaniuk, Prokulevich, Fomichev, Prozorov, Titok.

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Lagodich, A.V., Cherva, E.A., Shtaniuk, Y.V. et al. Construction of a vector system for molecular cloning in Bacillus subtilis and Escherichia coli . Mol Biol 39, 306–309 (2005). https://doi.org/10.1007/s11008-005-0043-7

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