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Interaction of RNA polymerase II general transcription complex proteins with oligoribonucleotides

  • Structural-Functional Analysis of Biopolymers and their Complexes
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Abstract

Saccharomyces cerevisiae RNA polymerase II general transcription complex proteins (RNA polymerase II, TBP, TFIIB, TFIIF, TFIIE, and TFIIH) were tested for interaction with oligoribonucleotides. Electrophoretic mobility shift assays showed that 32P-labeled CU-rich oligonucleotide p-17 (5′-ACUCUCUUCCG-CAUCGC- 3′) interacted with the proteins to yield three types of complexes, corresponding to three retardation bands. All complexes were RNA-specific, because [32P]p-17 was displaced by an excess of nonlabeled S. cerevisiae RNA and was not displaced by a 500-fold molar excess of a nonlabeled oligodeoxyribonucleotide. The complexes corresponding to the middle retardation band were specific for p-17, because [32P]p-17 was displaced almost completely by a 100-fold molar excess of nonlabeled p-17 and was not displaced by a 500-fold molar excess of an AU-rich oligoribonucleotide. The complexes corresponding to the upper retardation band were RNA-specific, and those corresponding to the lower band were assumed to result from nonspecific sorption of [32P]p-17 on a protein. Oligoribonucleotides and oligodeoxyribonucleotides did not compete for the binding with RNA polymerase II general transcription complex proteins.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    I. A. Drachkova, M. V. Lysova, O. V. Prokuda, A. A. Sokolenko, T. V. Arshinova, V. F. Kobzev & L. K. Savinkova

  2. Institute of Chemical Biology and Fundamental Medicine, Siberian Division, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    M. N. Repkova

  3. Novosibirsk State University, 630090, Novosibirsk, Russia

    V. I. Yamkovoi

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  2. M. V. Lysova
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  3. M. N. Repkova
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  4. O. V. Prokuda
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  5. A. A. Sokolenko
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  6. T. V. Arshinova
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  9. L. K. Savinkova
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Translated from Molekulyarnaya Biologiya, Vol. 39, No. 1, 2005, pp. 139–146.

Original Russian Text Copyright © 2005 by Drachkova, Lysova, Repkova, Prokuda, Sokolenko, Arshinova, Kobzev, Yamkovoi, Savinkova.

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Drachkova, I.A., Lysova, M.V., Repkova, M.N. et al. Interaction of RNA polymerase II general transcription complex proteins with oligoribonucleotides. Mol Biol 39, 123–129 (2005). https://doi.org/10.1007/s11008-005-0017-9

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  • DOI: https://doi.org/10.1007/s11008-005-0017-9

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