Abstract
Analysis of ESTs corresponding to human POLRMT for mitochondrial RNA polymerase suggested the existence of an alternative transcript. This mRNA, which contained 225 bp of the intron 1 proximal region, was detected in HeLa cells by reverse transcription—polymerase chain reaction. Alternative transcripts containing intron 1 were also found in mouse and rat cells. Moreover, a third transcript with an additional exon (142 bp), which originates from intron 2, was observed in mouse cells. In contrast to the major mRNA coding for the full-size RNA polymerase, the alternative transcripts code for a protein truncated at the N end and devoid of the mitochondrial targeting signal. The protein occurs exclusively in the nucleus and corresponds to new nuclear RNA polymerase IV, which was recently identified.
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Translated from Molekulyarnaya Biologiya, Vol. 39, No. 1, 2005, pp. 67–71.
Original Russian Text Copyright © 2005 by Kravchenko, Chumakov.
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Kravchenko, Y.E., Chumakov, P.M. Alternative transcripts of POLRMT gene coding for nuclear RNA polymerase IV. Mol Biol 39, 58–61 (2005). https://doi.org/10.1007/s11008-005-0008-x
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DOI: https://doi.org/10.1007/s11008-005-0008-x