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T-Cell Epitopes Based Vaccine Candidate’s Prediction for Treatment Against Burkholderia pseudomallei: Causative Agent of Melioidosis

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Abstract

Melioidosis is caused by the bacillus Burkholderia pseudomallei, this bacterium causes 165,000 new cases worldwide each year. Epitopes based vaccine a candidate of these bacterial species was identified by immunoinformatic study. Six epitopes VKAFRAAFAIPADVE, APVLIAVTAHTLTSK, GNPNFQAGDAARLIV, TLGAGINVDSTVGTS, LGAGINVDSTVGTSG, VKAFRAAFAIPADVE were linked to adjuvants, and linker molecules. Deep learning and artificial neural network tools were used to determine these epitopes, but structural prediction and validations shows multi-epitope vaccine was structurally and functionally sound. Molecular docking analysis shows interaction of multi-epitope vaccine and TLR-5 with ACE value of − 6.8 kcal/mol. Ramachandran plot shows greater than 97% amino acid residues are in allowed regions. The core objective of the current study is to craft multi-epitope vaccine against Burkholderia pseudomallei. This multi-epitope vaccine construct can be synthesised and validated by wet lab analysis in future studies, but in current study it shows good interaction patterns with TLR5 that will assist in its internalization. Here the validation of vaccine construct was conducted by simulation analysis that reveals stable interaction between ligand and receptor. Immunoinformatic studies not only save time but these methods were found to be economically beneficial.

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Acknowledgements

The authors acknowledge computational services and sound supervision provided throughout the research work by the Department of Biotechnology, Rama University Uttar Pradesh, Kanpur, India.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Engineering and Technology, Rama University, G.T. Road, Kanpur, 209217, India

    Komal Pal & Ajay Kumar

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  1. Komal Pal
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  2. Ajay Kumar
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Correspondence to Ajay Kumar.

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The author’s confirm that authors did not perform any experiments on human or animals as it is purely an in-silico study.

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Pal, K., Kumar, A. T-Cell Epitopes Based Vaccine Candidate’s Prediction for Treatment Against Burkholderia pseudomallei: Causative Agent of Melioidosis. Int J Pept Res Ther 28, 90 (2022). https://doi.org/10.1007/s10989-022-10400-6

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