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A New Broad-Spectrum Peptide Antibiotic Produced by Bacillus brevis Strain MH9 Isolated from Margalla Hills of Islamabad, Pakistan

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Abstract

The antimicrobial peptide from a bacterial strain is isolated from soil sample of Margalla Hills of Islamabad, Pakistan. The peptide is found to significantly inhibit the growth of both Gram-positive (Staphylococcus aureus ATCC 6538 and Micrococcus luteus ATCC 10240) and Gram-negative (Escherichia coli ATCC 25922 and Salmonella typhi ATCC 14028) bacteria as compared to gramicidin as standard. The bacterium is identified as Bacillus brevis strain MH9 based on phenotype and phylogenetic analysis. The antibacterial polypeptide was produced optimally at 35 °C after 48 h of growth, precipitated by 50 % ammonium sulphate, and further purified using HPLC. The sequential steps of purification decrease the peptide contents with prominent antibacterial activity. The peptide composed of 11 amino acid was further characterized by FT-IR and NMR. Results suggested that the peptide molecule is a novel antibacterial agent that is effective against both Gram-positive and Gram-negative bacteria. This study may have important implications for new peptide antibiotic that could be a new addition to treat infections.

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Authors and Affiliations

  1. Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, 60000, Pakistan

    Syed Aun Muhammad

  2. Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Islamabad, 44000, Pakistan

    Amjad Ali, Anam Naz, Afreenish Hassan & Saadia Andleeb

  3. Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad, Pakistan

    Naeem Riaz

  4. University College of Pharmacy, University of the Punjab, Lahore, Pakistan

    Syed Saeed-ul-Hassan

  5. Centre for Genomics and Applied Gene Technology, Institute of Integrative Omics and Applied Biotechnology (IIOAB), Nonakuri, Purba Medinipur, West Bengal, India

    Debmalya Barh

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  1. Syed Aun Muhammad
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  2. Amjad Ali
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  3. Anam Naz
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Correspondence to Amjad Ali.

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There was no funding available for this research. We acknowledge the administrative support of Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad and Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University Multan, Pakistan.

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Muhammad, S.A., Ali, A., Naz, A. et al. A New Broad-Spectrum Peptide Antibiotic Produced by Bacillus brevis Strain MH9 Isolated from Margalla Hills of Islamabad, Pakistan. Int J Pept Res Ther 22, 271–279 (2016). https://doi.org/10.1007/s10989-015-9508-2

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  • DOI: https://doi.org/10.1007/s10989-015-9508-2

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