Abstract
Semiconductor quantum dots have been used for labeling many biomacromolecules and small molecules, but it remains a challenge to couple it with short active peptides that play critical roles in many physiological processes. Several binding methods for QDs and short peptides have been reported, but all with some limitations in amino acid sequence. In this paper, we report a method for synthesis of quantum dots labeled short peptides that is appropriate to any short peptide. The quantum dots (CdTe)-labeled short peptides were verified and characterized by RP-HPLC. The QDs-labeled peptides were applied to monitor the specific binding between two immune peptides and T cell surface receptors. The quantum dots-labeled immune peptides provide a powerful method for studying immunological functions of these peptides, and an effective strategy for monitoring their complex modulating processes in vivo.
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References
Åkerman M. E., Chan W. C. W., Laakkonen P., Bhatia S. N., Ruoslahti E. (2002) PNAS 99: 12617–12621
Bailey R. E., Smith A. M., Nie S. (2004) Physica. E 25(1): 1–12
Bruchez Jr. M., Moronne M., Gin P., Weiss S., Paul Alivisatos A. (1998) Science 281: 2013–2016
Chan W. C., Nie S. (1998) Science 281(5385): 2016–2018
Chan, W. and White, P.: 2000, Fmoc Solid Phase Peptide Synthesis. Oxford University Press
Chan W. C., Maxwell D. J., Gao X., Bailey R. E., Han M., Nie S. (2002) Curr. Opin. Biotechnol. 13(1): 40–46
Gattas-Asfura, K. M. and Leblanc, R. M.: 2003, Chem. Commun. 2684–2685
Jaiswal J. K., Simon S. M. (2004) Trends Cell Biol. 14(9): 497–504
Lidke D. S., Nagy P., Heintzmann R., Arndt-Jovin D. J., Post J. N., Grecco H. E., Jares-Erijman E. A., Jovin T. M. (2004) Nat. Biotechnol. 22: 198–203
Winter J. O., Liu T. Y., Korgel B. A., Schmidt C. E. (2001) Adv. Mater. 13: 1673–1677
Zhang, C. Y., Ma, H., Ding, Y., Jin, L., Chen, D. Y. and Nie, S.: 2000, Analyst 125(6), 1029–1031
Zhang, H., Wang, L. P., Xiong, H. M., Hu, L. H., Yang, B., Li, W.: 2003a, Adv. Mater. 15(20), 1712–1715
Zhang H., Zhou Z., Yang B., Gao M. Y. (2003b) J. Phys. Chem. B 107: 8–13
Acknowledgments
This work was supported by a grant from the Special Funds for Major State Basic Research Projects (No. 2002CB613401).
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Qi, Y., Chen, J., Wang, L. et al. Synthesis of Quantum Dots Labeled Short Peptides and Imaging the T cell Surface Receptors with QDs-Labeled Peptides. Int J Pept Res Ther 13, 399–404 (2007). https://doi.org/10.1007/s10989-006-9047-y
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DOI: https://doi.org/10.1007/s10989-006-9047-y