Skip to main content
SpringerLink
Log in

Thermal stability of blood plasma proteins of breast cancer patients, DSC study

  • Published:
Journal of Thermal Analysis and Calorimetry Aims and scope Submit manuscript

Abstract

The thermodynamic stability of blood plasma main proteins of 17 patients (32–72-year-old women) with preliminary diagnosis of ductal carcinoma was studied. We had only one case, when a tissue with size <1.0 cm showed the same DSC results as blood plasma of healthy persons. In all other cases, the profile of curves and melting thermodynamic parameters of proteins were different from the norm. Depending on the disease stage, the albumin melting temperature (T m) increased by maximum 6 °C, the integral melting width (∆T m) increased by 40–100 %, the melting enthalpy (∆H m) did not change practically, and it was 23.0 J g−1 (protein). And what was more important, a new weakly expressed shoulder appeared in the range 57–58 °C when no rigid tissue mass was detected in breast yet. The shoulder that was expressed at stage I converted to a clear peak at stage III with melting parameters T m = 58 ± 1 °C and ∆T m = 2.5 ± 0.5 °C. It was supposed that the well-known cancer biomarkers—fibronectin and tenascins—could be the proteins that melted at 58 ± 1 °C. Estimation of these protein concentrations on the basis of DSC data gave us the values which varied within 500–850 µg mL−1 depending on the disease stages. After accumulation of a large number of experimental materials, we suppose that DSC can be used as one of the cheap and fast methods for the estimation of risk factors for breast cancer development when no rigid tissue mass is formed in mammary glands yet.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Provenzano PP, Inman DR, Eliceiri KW, Knittel JG, Yan L, Rueden CT, White JG, Keely PJ. Collagen density promotes mammary tumor initiation and progression, Collagen density promotes mammary tumor initiation and progression. BMC Med. 2008;6:11.

    Article  Google Scholar 

  2. Bertrand KA, Tamimi RM, Scott CG, Jensen MR, Pankratz VSH, Daniel V, Norman A, Couch F, Shepherd J, Fan B, Chen Y, Ma L, Beck AH, Cummings SR, Kerlikowske K, Vachon CM. Mammographic density and risk of breast cancer by age and tumor characteristics. Breast Cancer Res. 2013;. doi:10.1186/bcr3570.

    Google Scholar 

  3. Sala E, Solomon L, Warren R, McCann J, Duffy S, Luben R, Day N. Size, node status and grade of breast tumours: association with mammographic parenchymal patterns. Eur Radiol. 2000;10(1):157–61.

    Article  CAS  Google Scholar 

  4. Boyd NF, Guo H, Martin LJ, Sun L, Stone J, Fishell E, Jong RA, Hislop G, Chiarelli A, Minkin S, Yaffe MJ. Mammographic density and the risk and detection of breast cancer. N Engl J Med. 2007;356(3):227–36.

    Article  CAS  Google Scholar 

  5. Colpaert C, Vermeulen P, Van Marck E, Dirix L. The presence of a fibrotic focus is an independent predictor of early metastasis in lymph node-negative breast cancer patients. Am J Surg Pathol. 2001;25:1557–8.

    Article  CAS  Google Scholar 

  6. Hennighausen L, Robinson GW. Information networks in the mammary gland. Nat Rev Mol Cell Biol. 2005;6(9):715–25.

    Article  CAS  Google Scholar 

  7. Monaselidze J, Kiladze M, Tananashvili D, Barbakadze S, Naskidashvili A, Khizanishvili A, Kvavadze R, Majagaladze G. Free and bound water influence on spirulina platensis survival. J Therm Anal Calorim. 2006;84(3):613–8.

    Article  CAS  Google Scholar 

  8. Monaselidze J, Barbakadze S, Kvirikashvili S, Majagaladze G, Khachidze D, Topchishvili L. Thermal characteristics of spirulina platensis cells under non-growing conditions at various values pH medium. Biomacromolecules. 2002;3(4):783–6.

    Article  CAS  Google Scholar 

  9. Monaselidze J, Kalandadze Y. Thermodynamic properties of serum and plasma of patients sick with cancer. High Temp High Press. 1997;29:677–80.

    Article  CAS  Google Scholar 

  10. Privalov PL. Stability of proteins. Proteins which do not present a single cooperative system. Adv Protein Chem. 1982;35:1–104.

    Article  CAS  Google Scholar 

  11. Privalov PL. Microcalorimetry of macromolecules: protein folding, multidomain proteins. In: Wiley J, editor USA; 2012 p. 225–72.

  12. Garbett NC, Miller JJ, Jenson AB, Chaires JB. Calorimetry outside the box: a new window into the plasma proteome. Biophys J. 2008;94(4):1377–83.

    Article  CAS  Google Scholar 

  13. Dafforn TR, Mahadeva R, Elliott PR, Sivasothy P, Lomas DA. A kinetic mechanism for the polymerization of alpha1-antitrypsin. J Biol Chem. 1999;274(14):9548–55.

    Article  CAS  Google Scholar 

  14. Khachidze DG, Monaselidze DR. Microcalorimetric study of human blood serum. Biofizika. 2000;45(2):320–4.

    CAS  Google Scholar 

  15. Monaselidze J, Tevdoradze T, Zibzibadze M, Alibegashvili M, Ramishvili L, Gordeziani M, Kotrikadze N. The study of blood serum proteins in patients with mammary gland tumours. J Biol Phys Chem. 2012;1:19–22.

    Article  Google Scholar 

  16. Monaselidze J, Lezhava T, Khachidze D, Gorgoshidze M, Kiladze M, Lomidze E, Tvauri G, Barbakadze Sh, Kikaleishvili L, Ramishvili M, Nemsadze G. The possibilities of DSC in breast cancer study and diagnostics. International scientific conference on physical research methods in medicine, Tbilisi. 2011; Proceedings I p.177–80.

  17. Monaselidze J, Kalandadze Ya, Tofuridze I, Gadabadze M, Tsertsvadze T. Scanning microcalorimetry of human plasma/serum of patient with various forms of leukemia and immunodeficiency virus. 1996; 14th IUPAC conference on chemical thermodynamics. Aug 25–30, Osaka, Japan, S7–26 p 22.

  18. Khachidze DG, Monaselidze DR. Independent denaturation of albumin and globulin in human blood serum. Biofizika. 2000;45(2):325–8.

    CAS  Google Scholar 

  19. Zapf I, Fekecs T, Ferencz A, Tizedes G, Pavlovics G, Kálmán E, Lőrinczy D. DSC analysis of human plasma in breast cancer patients. Thermochim Acta. 2011;524(1–2):88–91.

    Article  CAS  Google Scholar 

  20. Jennifer J, Choate MD, Deane F, Mosher MD. Fibronectin concentration in plasma of patients with breast cancer, colon cancer, and acute leukemia. Cancer. 1983;51(6):1142–7.

    Article  Google Scholar 

  21. Guttery DS, Hancox RA, Mulligan KT, Hughes S, Lambe SM, Pringle HJ, Walker RA, Jones LJ, Shaw JA. Association of invasion-promoting tenascin-C additional domains with breast cancers in young women. Breast Cancer Res. 2010;. doi:10.1186/bcr2618.

    Google Scholar 

  22. Brellier F, Martina E, Degen M, Heuzé-Vourch N, Petit A, Kryza T, Courty Y, Terracciano L, Ruiz C, Chiquet-Ehrismann R. Tenascin-W is a better cancer biomarker than tenascin-C for most human solid tumors. BMC Clin Pathol. 2012;. doi:10.1186/1472-6890-12-14.

    Google Scholar 

  23. Mosher DF. Plasma fibronectin concentration: a risk factor for arterial thrombosis? Arterioscler Thromb Vasc Biol. 2006;26:1193–95.

    Article  CAS  Google Scholar 

  24. Góralski P, Rogalińska M, Błoński JZ, Pytel E, Robak T, Kiliańska ZM, Piekarski H. The differences in thermal profiles between normal and leukemic cells exposed to anticancer drug evaluated by differential scanning calorimetry. J Therm Anal Calorim. 2014;118(2):1339–44.

    Article  Google Scholar 

  25. Szalai Z, Molnár TF, Lőrinczy D. Differential scanning calorimetry (DSC) of blood serum in chronic obstructive pulmonary disease (COPD). J Therm Anal Calorim. 2013;113(1):259–64.

    Article  CAS  Google Scholar 

  26. Moezzi M, Ferencz A, Lőrinczy D. Evaluation of blood plasma changes by differential scanning calorimetry in psoriatic patients treated with drugs. J Therm Anal Calorim. 2014;116(2):557–62.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Tbilisi State Medical University, Tbilisi, Georgia

    L. Kikalishvili & M. Ramishvili

  2. K. Madichi Mammalogy Centre, Tbilisi, Georgia

    G. Nemsadze & P. Khorava

  3. Tbilisi State University, Tbilisi, Georgia

    T. Lezhava

  4. E. Andronikashvili Institute of Physics, STU, Tbilisi, Georgia

    M. Gorgoshidze, M. Kiladze & J. Monaselidze

Authors
  1. L. Kikalishvili
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Ramishvili
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Nemsadze
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Lezhava
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. Khorava
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Gorgoshidze
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. Kiladze
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. J. Monaselidze
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Monaselidze.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kikalishvili, L., Ramishvili, M., Nemsadze, G. et al. Thermal stability of blood plasma proteins of breast cancer patients, DSC study. J Therm Anal Calorim 120, 501–505 (2015). https://doi.org/10.1007/s10973-015-4426-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10973-015-4426-2

Keywords

Search

Navigation