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Thermokinetic responses of the metabolic activity of Staphylococcus lentus cultivated in a glucose limited mineral salt medium

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Abstract

Biocalorimetric experiments were performed to investigate the metabolic thermal responses of the halotolerant species Staphylococcus lentus in glucose limited mineral salt medium. Growth factors were optimized in both shaker flask and calorimetric experiments. A limiting value of 5 g/L glucose was found to be the optimum for S. lentus growth. The heat flux profiles, OUR, biomass growth, and substrate depletion profiles were compared to deduce the metabolic activity of S. lentus. Shifts in heat flux due to the shifts in substrate uptake and three distinct phases of growth are noticeable in heat profile curves. Respirogram (OUR) and heat profiles are seen to follow the biomass growth curve. Oxycalorific coefficient is validated with the theoretical studies and those noticed in published literature.

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Abbreviations

LPM:

Liters per minute

S :

Substrate concentration (g/L)

R :

Correlation coefficient

k s :

Half-saturation constant for growth (g/L)

μ:

Specific growth rate (h−1)

μmax :

Maximum specific growth rate (h−1)

Y Q/S :

Heat yield due to substrate depletion (kJ/g)

Y Q/X :

Heat yield due to biomass growth (kJ/g)

Y Q/O :

Oxycalorific coefficient (kJ/mol)

T r :

Reactor temperature (°C)

T j :

Temperature of jacket fluid (silicone oil) (°C)

Q acc :

Heat accumulation (Q)

Q est :

Heat generation rates in reaction medium (Q)

References

  1. Senthilkumar S, Surianarayanan M, Sushleela R. Biocalorimetric studies of the metabolic activity of Pseudomonas aeroginasa aerobically grown in glucose limited complex growth medium. Biosci Biotechnol Biochem. 2008;72:936–42.

    Article  Google Scholar 

  2. Higuera-Guisset J, Rodriguez-Viejo J. Calorimetry of microbial growth using a thermopile based microreactor. Thermochimica Acta. 2005;427:187–91.

    Article  CAS  Google Scholar 

  3. Ligthart J, Daverio E. Application of calorimetric measurements for biokinetic characterization of nitrifying population in activated sludge. Water Res. 2003;37:2723–31.

    Article  Google Scholar 

  4. Vonstockar U, Duboc P, Menoud L, Marison IW. On-line calorimetry as a technique for process monitoring and control in biotechnology. Thermochimica Acta. 1997;300:225–36.

    Article  CAS  Google Scholar 

  5. Liu JS, Marison IW, Von Stockar U. Microbial growth by a net heat up-take: a calorimetric and thermodynamic study on acetotrophic methanogenesis by Methanosarcina bakeri. Biotechnol Bioeng. 2001;75:170–80.

    Article  CAS  Google Scholar 

  6. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem. 1959;31:426–8.

    Article  CAS  Google Scholar 

  7. Singh V. On-line measurement of oxygen uptake in cell culture using the dynamic method. Biotechnol Bioeng. 1996;52:443–8.

    Article  CAS  Google Scholar 

  8. Surianarayanan M, Balaji D, Senthilkumar S, Asit Baran M. Batch kinetics on growth of salt tolerant Pseudomonas aeroginasa secreting in a biocalorimeter. Biotechnol Bioprocess Eng. 2010;15:670–5.

    Article  Google Scholar 

  9. Bailey J, Ollis F. Biochemical engineering fundamentals. New York: McGraw-Hill; 1986. p. 383–6.

    Google Scholar 

  10. Robinson JA, Tiedj JM. Nonlinear estimation of Monod growth kinetic parameters from a single substrate depletion curve. Appl Environ Microbial. 1983;45:1453–8.

    CAS  Google Scholar 

  11. Senthilkumar S, Surianarayanan M, Swaminathan G. Biocalorimetric and respirometric studies on biological treatment of tannery saline wastewater. Appl Microb Biotechnol. 2008;78:249–55.

    Article  CAS  Google Scholar 

  12. Senthilkumar S, Surianaryanan M, Madhu B. Biocalorimetric and respirometric studies on metabolic activity of aerobically grown batch culture of Pseudomonas aeruginosa. Biotechnol Bioprocess Eng. 2007;12:340–7.

    Article  Google Scholar 

  13. Birou B, Marison IW, Von stockar U. Calorimetric investigation of aerobic fermentations. Biotechnol Bioeng. 1987;30:650–60.

    Article  CAS  Google Scholar 

  14. Surianarayanan M, Senthilkumar S. Bioenergetics studies on aerobic growth of Pseudomonas aeroginasa in a single substrate media. J Chem Technol Biotechnol. 2009;84:1234–9.

    Article  Google Scholar 

  15. Von Stockar U, Marison IW. The use of calorimetry in biotechnology. Adv Biochem Eng Biotechnol. 1989;40:93–136.

    Google Scholar 

  16. Cooney CL, Wang D, Mateles RI. Biotechnol Bioeng. 1968;11:269.

    Article  Google Scholar 

Download references

Acknowledgements

One of the authors (Mr S Sudharshan) is grateful to CSIR-New Delhi for the award of Senior Research Fellowship. The authors are thankful to Prof NR Rajagopal for his encouragement.

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Authors and Affiliations

  1. Chemical Engineering Department, Central Leather Research Institute (CLRI), Adyar, Chennai, 600 020, Tamilnadu, India

    Sudharshan Sekar, Surianarayanan Mahadevan, Sathish Sundar Dhilip Kumar & Asit Baran Mandal

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  1. Sudharshan Sekar
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  2. Surianarayanan Mahadevan
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  3. Sathish Sundar Dhilip Kumar
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  4. Asit Baran Mandal
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Correspondence to Surianarayanan Mahadevan.

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Sekar, S., Mahadevan, S., Kumar, S.S.D. et al. Thermokinetic responses of the metabolic activity of Staphylococcus lentus cultivated in a glucose limited mineral salt medium. J Therm Anal Calorim 104, 149–155 (2011). https://doi.org/10.1007/s10973-010-1121-1

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