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Structural characteristics and cyclic voltammetric behaviour of Sonogel-Carbon tyrosinase biosensors. A detailed comparative study of three immobilization matrixes

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Abstract

Structural characteristics an cyclic voltammetry of three amperommetric biosensors based on immobilization of tyrosinase on a Sonogel-Carbon electrode for detection of phenols are described. Cyclic voltammetry was applied to study the electrochemical behaviour of the electrode and the electrochemical reaction on the electrode surface. Scanning electron microscopy, X-ray energy dispersive spectroscopy and atomic force microscopy were used for the structure characterization of the electrode surface, enzyme film and polymers coatings. The influence of additive-protective polymers, such as polyethylene glycol and perfluorinated-Nafion ion-exchanger on the surface of the biosensor were explored.

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Acknowledgements

The authors are grateful to FEDER (Fondo Europeo de Desarrollo Económico y Regional) and the Ministerio de Educación y Ciencia of Spain (Project CTQ2004-03708/BQU) and to the Junta de Andalucía for financial support, and Agencia Española de Cooperación Internacional (AECI) through a grant to Mohammed ElKaoutit.

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Authors and Affiliations

  1. Departamento de Química Analítica, Facultad de Ciencias, Universidad de Cádiz, Polígono Rio San Pedro, s/n, Apdo. 40, 11510, Puerto Real, Cadiz, Spain

    Mohammed ElKaoutit, Ignacio Naranjo-Rodríguez & José Luis Hidalgo-Hidalgo-de-Cisneros

  2. Departement de Chimie, Equipe de Recherche Electrochimie et Systèmes Interfaciaux, Faculté des Siences de Tétouan, Université Abdelmalek Essaâdi, B.P. 2121, M’Hannech II, 93002, Morocco

    Khalid Riffi Temsamani

  3. Departamento de Física de la Materia Condensada, Facultad de Ciencias, Universidad de Cádiz, Apdo. 40, 11510, Puerto Real, Cadiz, Spain

    Manuel Domínguez

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  1. Mohammed ElKaoutit
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  2. Ignacio Naranjo-Rodríguez
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  3. Khalid Riffi Temsamani
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  4. Manuel Domínguez
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  5. José Luis Hidalgo-Hidalgo-de-Cisneros
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Correspondence to Ignacio Naranjo-Rodríguez.

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ElKaoutit, M., Naranjo-Rodríguez, I., Temsamani, K.R. et al. Structural characteristics and cyclic voltammetric behaviour of Sonogel-Carbon tyrosinase biosensors. A detailed comparative study of three immobilization matrixes. J Sol-Gel Sci Technol 45, 157–163 (2008). https://doi.org/10.1007/s10971-007-1671-2

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