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RIKEN structural genomics beamlines at the SPring-8; high throughput protein crystallography with automated beamline operation

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Journal of Structural and Functional Genomics

Abstract

RIKEN Structural Genomics Beamlines, BL26B1 & BL26B2 at the SPring-8, have been constructed for the structural genomics research. The main feature of the beamline is full automation of the successive data collections to maximize the beam-time efficiency. The beamline optics adopted a standard design commonly used for the SPring-8 bending magnet beamlines. Beamline instruments are operated by centralized control system through the computer network to achieve the automatic operation. The core part of the beamline development is a sample management system composed of sample changer robots for laboratory and beamline, and a networked sample database. BL26B1 has started user operation, and the automatic operation with the sample management system has been implemented at BL26B2.

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Acknowledgements

We thank Yuji Fukumoto and Hironori Murakami as beamline operation staffs. Yukito Furukawa and other staffs at SPring-8 Beamline Control Group are greatly appreciated for providing the control system and device servers. Thanks are owed to Atsushi Nisawa for valuable suggestions and cooperation on beamline tuning. Thanks to Shunji Goto and Kunikazu Takeshita at Japan Synchrotron Radiation Research Institute (JASRI) for useful advice and cooperation in beamline construction. We also thank Tetsuya Ishikawa and Masashi Miyano at RIKEN/SPring-8 for valuable supports and discussions. A part of this work was supported by National Project on Protein Structural and Function Analysis by MEXT of Japan.

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Author notes

  1. Hiroyuki Kanda

    Present address: Rigaku co., Matsubara, Akishima, Tokyo, 196-8666, Japan

  2. Koh Ida

    Present address: Kitasato University, Kitasato, Sagamihara, Kanagawa, 228-8555, Japan

Authors and Affiliations

  1. SPring-8/RIKEN, Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan

    Go Ueno, Hiroyuki Kanda, Koh Ida, Takashi Kumasaka & Masaki Yamamoto

  2. Rigaku co., Matsubara, Akishima, Tokyo, 196-8666, Japan

    Go Ueno

  3. PharmAxess, Inc., CAST, Kouto, Kamigori, Ako, Hyogo, 678-1205, Japan

    Raita Hirose

  4. Yokohama City University, Suehiro, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan

    Koh Ida

  5. Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan

    Takashi Kumasaka

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  1. Go Ueno
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  2. Hiroyuki Kanda
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  3. Raita Hirose
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  4. Koh Ida
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  5. Takashi Kumasaka
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  6. Masaki Yamamoto
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Correspondence to Go Ueno.

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Ueno, G., Kanda, H., Hirose, R. et al. RIKEN structural genomics beamlines at the SPring-8; high throughput protein crystallography with automated beamline operation. J Struct Funct Genomics 7, 15–22 (2006). https://doi.org/10.1007/s10969-005-9005-5

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  • DOI: https://doi.org/10.1007/s10969-005-9005-5

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