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Construction of Deino-flr-2 radiation-tolerant genetically engineered strain containing flr-2 fluoride-tolerant gene and its enrichment behavior for U(VI)

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Abstract

In order to solve the problem of lack of resistance of active microorganisms to the complex ion environment in the treatment of uranium-containing wastewater, the Deino-flr-2 radiation-resistant genetically engineered bacteria containing flr-2 fluorine-resistant gene was constructed and characterized. Utilizing the reductase activity of Deinococcus radiodurans (DR) itself and the enrichment of U(VI) by the surface active functional groups of the bacteria, the treatment of simulated low-concentration uranium-containing wastewater will be realized. In the most favorable conditions, accounting for approximately 90% of U(VI) was removed. The calculated data disclosed that the enrichment process of U(VI) by Deino-flr-2 well fit with the pseudo-second-order kinetic and Freundlich isotherm model.

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Acknowledgements

The financial support from the National Natural Science Foundation of China (CN) (Grants 11705085) and the Hunan Province Natural Science Foundation of China (Grants 2020JJ6050 and 2020JJ4077) are gratefully acknowledged.

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Author notes

  1. Jiaqi Luo and Qiqi Zhu have contributed equally to this work and should be considered co-first authors.

Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, University of South China, Hengyang, 421001, Hunan, People’s Republic of China

    Jiaqi Luo & Guowen Peng

  2. School of Public Health, University of South China, Hengyang, 421001, Hunan, People’s Republic of China

    Qiqi Zhu, Shanshan Li & Fangzhu Xiao

  3. Hunan Province Engineering Technology Research Center of Uranium Tailings Treatment, University of South China, Hengyang, 421001, Hunan, People’s Republic of China

    Fang Chen & Guowen Peng

  4. Hunan Engineering Research Center for the Safety Control and Recycling of Radioactive Heavy Metal Pollutants, University of South China, Hengyang, 421001, Hunan, People’s Republic of China

    Fangzhu Xiao & Guowen Peng

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  1. Jiaqi Luo
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Luo, J., Zhu, Q., Li, S. et al. Construction of Deino-flr-2 radiation-tolerant genetically engineered strain containing flr-2 fluoride-tolerant gene and its enrichment behavior for U(VI). J Radioanal Nucl Chem 328, 1265–1278 (2021). https://doi.org/10.1007/s10967-021-07697-w

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  • DOI: https://doi.org/10.1007/s10967-021-07697-w

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