Abstract
The aim of this study was to synthesize hyaluronic acid–tyramine (HA–Tm) conjugate and label it with radioisotopes for in vivo biodistribution study. Radiolabeling of HA–Tm was carried out using 125I and 131I and the observed radiochemical yield were 96 and 98 % respectively. Biodistribution study of radiolabeled HA–Tm was performed with normal Balb/c mice via oral administration. The uptake values of [131I]HA–Tm in organs were measured by gamma-counter at different time points. Biodistribution results showed that most radioactivities were observed in the gastrointestinal tract at initial time point. A small part of [131I]HA–Tm was then absorbed into the bloodstream from the small intestine and distributed in some organs. SPECT/CT images of orally administered [125I]HA–Tm were well matched with the biodistribution data. The results in the present study would provide an efficient radiolabeling method of hyaluronic acid and also present quantitative organ distributions of orally administered HA.
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Acknowledgments
This work was supported by the Nuclear Research Development Project from the Korean Ministry of Science, ICT and Future Planning (Grant no. 2012M2A2A601135). This work was supported in part by the National Research Foundation of Korea Grant funded by the Korea government (Grant no. 2012M2B2B1055244) and Korea Atomic Energy Research Institute (527200-14).
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So-Young Ma and You Ree Nam have contributed equally to this study.
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Ma, SY., Nam, Y.R., Jeon, J. et al. Simple and efficient radiolabeling of hyaluronic acid and its in vivo evaluation via oral administration. J Radioanal Nucl Chem 305, 139–145 (2015). https://doi.org/10.1007/s10967-015-3986-8
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DOI: https://doi.org/10.1007/s10967-015-3986-8