Skip to main content
SpringerLink
Log in

Detection of additional impurities in the UV-chromatogram of l-[S-methyl-11C]methionine

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

Samples of l-[S-methyl-11C]methionine prepared by on-column [11C]methylation on a C18 cartridge were analyzed by HPLC under different conditions in order to explain some anomalies observed in the UV-chromatograms. By so doing, two new unlabelled impurities were found and identified as iodide and homocystine. Their amounts are still compatible with the safe human use of the radiotracer solution. Nevertheless, if needed, iodide can be totally removed by elution through an anion-exchange resin, while homocystine can be reduced by either decreasing the amount of precursor used in the synthesis or preventing its air oxidation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Nakada K (2004) The role of methionine PET in oncology. Int Congress Series 1264:88–94

    Article  CAS  Google Scholar 

  2. Yamane T, Sakamoto S, Senda M (2010) Clinical impact of 11C-methionine PET on expected management of patients with brain neoplasm. Eur J Nucl Med 37:685–690

    Article  Google Scholar 

  3. Pascali C, Bogni A, Iwata R, Decise D, Crippa F, Bombardieri E (1999) High efficiency preparation of l-[S-methyl-11C]methionine by on-column [11C]methylation on C18 Sep-Pak. J. Labelled Compd Radiopharm 42:715–724

    Article  CAS  Google Scholar 

  4. Mitterhauser M, Wadsak W, Krcal A, Schmaljohann J, Eidherr H, Schmid A, Viernstein H, Dudczak R, Kletter K (2005) New aspects on the preparation of [11C]methionine—a simple and fast online approach without preparative HPLC. Appl Radiat Isot 62:441–445

    Article  CAS  Google Scholar 

  5. Gomez V, Gispert JD, Amador V, Llop J (2008) New method for routine production of l-[methyl-11C]methionine: in loop synthesis. J Label Compd Radiopharm 51:83–86

    Article  CAS  Google Scholar 

  6. Bogni A, Bombardieri E, Iwata R, Cadini L, Pascali C (2003) Stability of l-[S-methyl-11C]methionine solutions. J Radioanal Nucl Chem 256:109–203

    Article  Google Scholar 

  7. Schmitz F, Plenevaux A, Del-fiore G, Lemaire C, Comar D, Luxen A (1995) Fast routine production of l-[11C-methyl]methionine with Al2O3/KF. Appl Radiat Isot 46:893–897

    Article  CAS  Google Scholar 

  8. Berridge MS, Cassidy EH, Miraldi F (1995) [11C]Acetate and [11C]methionine: improved syntheses and quality control. Appl Radiat Isot 46:173–175

    Article  CAS  Google Scholar 

  9. L-Methionine ([11C]methyl) injection (2008) European Pharmacopoeia 6.0 edn, 1001-1003

  10. Laurie AJ, Lyon SG, Lasser EC (1992) Contrast material iodides: potential effects on radioactive iodine thyroid uptake. J Nucl Med 33:237–238

    CAS  Google Scholar 

  11. Product information Pralidoxime Iodide (P.A.M.) Injection (2008) Version 05, Phebra Pty Ltd

  12. Duerre JA, Miller CH (1966) Preparation of l-homocysteine from l-homocysteine thiolactone. Anal Biochem 17:310–315

    Article  CAS  Google Scholar 

  13. Korsakov MV, Kisselev MY, Solovyov D, Horti AG, Vasilev DA (1991) Robotic synthesis of [carbon-11]methionine. In: Proceedings of the IVth international workshop on targetry and target chemistry, Villigen, Switzerland, 9–12 September 1991

  14. Mudd SH, Finkelstein JD, Refsum H, Ueland PM, Malinow MR, Lentz SR, Jacobsen DW, Brattstrom L et al (2000) Homocysteine and its disulfide derivatives. Arterioscler Thromb Vasc Biol 20:1704–1706

    CAS  Google Scholar 

  15. Ragone R (2002) Homocystine solubility and vascular disease. FASEB J 16:401–404

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the Project 5‰ “Development of Radiopharmaceuticals”, the AIRC Project “Molecular and Cellular Imaging of Cancer” and the Project PIO INT Ministero Lavoro.

Author information

Author notes

  1. O. Crispu

    Present address: DANI Instrument Spa, V. Brianza, 87, 20093, Cologno Monzese Milan, Italy

Authors and Affiliations

  1. Fondazione IRCCS Istituto Nazionale dei Tumori, V. Venezian, 1, 20133, Milan, Italy

    C. Pascali, A. Bogni, C. Cucchi, L. Laera, G. Maiocchi, F. Crippa & E. Bombardieri

Authors
  1. C. Pascali
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Bogni
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Cucchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Laera
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. O. Crispu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. Maiocchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. F. Crippa
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. E. Bombardieri
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. Pascali.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pascali, C., Bogni, A., Cucchi, C. et al. Detection of additional impurities in the UV-chromatogram of l-[S-methyl-11C]methionine. J Radioanal Nucl Chem 288, 405–409 (2011). https://doi.org/10.1007/s10967-011-0995-0

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10967-011-0995-0

Keywords

Search

Navigation