Skip to main content
SpringerLink
Log in

Comparison study between direct and indirect labeling of estradiol for radioimmunoassay purpose

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

In the present study, estradiol (E2) which contains an aromatic phenol ring in its chemical structure was chosen for direct and indirect labeling techniques using 125I and chloramine-T oxidation method. In direct technique the hydrogen atom in phenol ring was replaced by a radioiodine atom directly, which in indirect one, histidine methyl ester (HME) was labeled firstly with 125I using chloramine-T method then conjugated with E2. The 125I-labeled materials were purified using HPLC technique. The comparison study between the two obtained tracers was carried out in terms of radiochemical purity, binding percent, specific activity, non specific binding, binding displacement, shelf life using a radioimmunoassay (RIA) with specific antibody. The results indicated that indirect iodination of estradiol will cause significant increasing in studied parameters when combined with specific antibody than direct one.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11

Similar content being viewed by others

References

  1. El-Shaboury, G., El-Tawoosy, M.: Synthesis of radioiodinated 4-[*I] iodantipyrine via exchange. J. Radioanal. Nucl. Chem. 252(1), 109–114 (2002)

    Article  CAS  Google Scholar 

  2. El-Shaboury, G., Farah, K., El-Tawoosy, M.: Nucleophilic radioiodination of 6-bromocholesterol via non-isotopic exchang reaction in molton. J. Radioanal. Nucl. Chem. 249(3), 535–540 (2001)

    Article  CAS  Google Scholar 

  3. El-Mouty, N.R.A., Attallah, K.M., El-Tawoosy, M., El-Kolaly, M.T.: Radioiodination and Biological Evaluation of Atenolol as a possible Cardiac Imaging agent. Arab. J. Nucl. Sci. Appl. 41(3), 29–37 (2008)

    Google Scholar 

  4. Stupnicki, R., Kowalezyk-Przeewloka, T., Kula, E., Kasongo, L.Y.: HME—a novel derivative for direct iodination in steroid. J. Steroid Biochem. 28(6), 663–667 (1987)

    Article  CAS  Google Scholar 

  5. Malmquist, M., Thorell, J.I.: Direct radioiodination of estradiol for a sensitive radioimmunoassay. J. Clin. Endocrynol. Metab. 49, 655–657 (1979)

    Article  CAS  Google Scholar 

  6. Niwender, G.D., Akbar, A.M., Nett, J.M.: Preparation of directly iodinated steroid hormones and related coumpounds. In: Methods in Enzymology. Hormone Action, Part A: Steroids Hormones, vol. 36, pp. 16–21. Academic Press (1975)

  7. Althouse, R., Wald, N.: Survival and handicap of infants with spina bifida. J. Arch. Dis. Child. 55, 845–850 (1981)

    Article  Google Scholar 

  8. Grundy, E.E., Simth, A.M.: Preparation of [T3+T4] free pooled plasma. Ann. Clin. Biochem. 32, 494–495 (1986)

    Google Scholar 

  9. El-Kolaly, M.T., Mehany, N.L., Hassan, S.E.M., Ayyoub, S.M.: Development, optimization and validation of one-step TSH IRMA using solid phase anti-TSH magnetic particles. Arab. J. Nucl. Sci. Appl. 39(2), 319–333 (2006)

    Google Scholar 

  10. El-Kolaly, M.T., Mehany, N.L., Ayyoub, S.M., Hassan, S.E.M.: Solid phase radioimmunoassay for measuring serum triiodothyronine and thyroxine using different preparation of their labeled hormones. Arab. J. Nucl. Sci. Appl. 38(3), 229–239 (2005)

    Google Scholar 

  11. El-Kolaly, M.T., Mehany, N.L., Ragab, M.T., El-Mohty, A.A.: Studies on Local preparation of double antibody radioimmunoassay for determination of alphafeto protein as a tumor marker. Arab. J. Nucl. Sci. Appl. 34(2), 295–315 (2001)

    Google Scholar 

  12. Mehany, N.L., El-Kolaly, M.T., El-Hashash, M.A., Sallam, Kh. M.: Liquid phase radioimmunoassay system for determination of progesterone in human serum using different radiolabeled tracers. Arab. J. Nucl. Sci. Appl. 40(1), 299–311 (2007)

    Google Scholar 

  13. Salacinski, P.R.P., Mclean, C., Sykes, J.E.C., Clement-Jones, V.V., Lowary, P.J.: Iodination of protein, glycoproteins and peptides using solid phase oxidizing agents 1,3,4,6-tetra chloro-3a,6a-diphenyleglycoluril. Anal. Biochem. 117, 136–141 (1981)

    Article  CAS  Google Scholar 

  14. Nemeth, J., Oroszei, G., Jakab, B., Magyarlaki, M., Szilvassy, Z., Roth, E., Farkas, B.: 125I-labeling and purification of peptide hormones and bovine serum albumin. J. Radioanal. Nucl. Chem. 251(1), 129–133 (2002)

    Article  CAS  Google Scholar 

  15. Byszewska, E.S., Markiewicz, A.: New RIA kit for determination of progesterone in Cows milk. J. Immunoass. Immunochem. 27(3), 279–288 (2006)

    Article  Google Scholar 

  16. Corrie, J.E.T., Hunter, W.M., Macpherson, J.S.: The provision of 125I-labelled tracers for radioimmunoassay of haptens: a general approach? J Immunol Methods 51(2), 159–166 (1982)

    Article  CAS  Google Scholar 

  17. Karir, T., Samuel, G., Kothari, K., Sivaparasad, N., Venkatesh, M.: J. Studies on the infulence of the structural modifications in the tracer of immunoassay of progesterone. Immunoass. Immunochem. 27(2), 151–171 (2006)

    Article  CAS  Google Scholar 

  18. Karir, T., Pal, N., Sivaparasad, N.: Radioimmunoassay kit formulation and its validation for serum progesterone using progesterone radiotracer purified by gel filtration. J. Radioanal. Nucl. Chem. 256, 127–131 (2003)

    Article  CAS  Google Scholar 

  19. Nomura, M., Imai, M., Usuda, S.: Solid phase radioimmunoassay of serum estradiol. J. Immunol. Methods 56, 13–17 (1983)

    Article  CAS  Google Scholar 

  20. Kordi, A., Banerjee, S., Pillai, M.R.A., Venkatesh, M.: Preparation and evaluation of 125I-aflatoxin B1. J. Radioanal. Nucl. Chem. 250(2), 231–236 (2001)

    Article  Google Scholar 

  21. Abdel-Fattah, A.A., Moustafa, K.A., El-Kollay, M.T.: Chemical kinetics of progesterone radioimmunoassay system. Arab. J. Nucl. Sci. Appl. 37(2), 101–110 (2004)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Labeled Compounds, Hot Laboratory Centre, Atomic Energy Authority, Post No. 13759, Cairo, Egypt

    Kh. M. Sallam & N. L. Mehany

Authors
  1. Kh. M. Sallam
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. L. Mehany
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kh. M. Sallam.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sallam, K.M., Mehany, N.L. Comparison study between direct and indirect labeling of estradiol for radioimmunoassay purpose. J Radioanal Nucl Chem 281, 329–337 (2009). https://doi.org/10.1007/s10967-009-0013-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10967-009-0013-y

Keywords

Search

Navigation