Abstract
The synthesis of two isotopomers of L-DOPA, the precursors for labeled dopamine is reported. The ring labeled with deuterium or tritium [2′,5′,6-2H3]-L-DOPA, and [2′,5′,6-3H3]-L-DOPA were obtained using acid catalyzed isotopic exchange between L-DOPA and heavy or tritiated water, respectively. Their derivatives, i.e., [2′,5′,6-2H3], and [2′,5′,6-3H3]-dopamine were obtained by enzymatic decarboxylation of deuterated or tritiated isotopomers of L-DOPA using enzyme tyrosine decarboxylase (EC 4.1.1.25).
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Pająk, M., Kańska, M. Enzymatic synthesis of dopamine ring labeled with hydrogen isotopes. J Radioanal Nucl Chem 279, 455–458 (2009). https://doi.org/10.1007/s10967-008-7335-z
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DOI: https://doi.org/10.1007/s10967-008-7335-z