Abstract
Nanogels have become an important topic of interdisciplinary research, especially in the fields of polymer chemistry, physics, materials science, pharmacy, and medicine where their small dimensions prove highly advantageous. One of the most important areas of research and development concerning these gels is in drug delivery applications. Nanogels could potentially revolutionize conventional therapy and diagnostic methods because of their superior effectiveness over their macro-sized counterparts in almost all therapeutic areas. Current strong interests in this class of material have driven many studies to discover novel production methods and new areas of application in this area. Therefore, it is important to keep abreast of the development of these gels. In this review, we aim to cover the basic aspects of organic nanogels including their definition, classification, and synthesis methods.
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Introduction
Polymeric submicron gels (nanogels and microgels) were once regarded as unwanted by-products of the polymerization process. These gels have now become increasingly important in many areas of research due to their diverse and useful properties. These properties include a high loading capacity for therapeutics such as drugs [1], imaging agents, proteins [2], DNA [3], and RNA [4]. These gels also have space for ligand attachments, high mechanical strength [5] that affords a higher resistance to degradation, and the ability to prolong the circulation period of cargo loading in the blood stream [6,7,8]. The gels’ small size and thus enhanced permeability [9] allows for efficient delivery of drugs to tumor sites and enables them to act as a protective layer for payloads [10,11,12]. These minute gels are now being widely used in many fields that include chemistry, physics, medical, pharmaceutical, and materials science. Such strong interests in this class of material have been the main drive for many studies to discover novel production methods and new areas of application in this area. Therefore, it is important to keep abreast of the development of these gels. In this review, we aim to cover the basic aspects of organic nanogels including their definition, classification, and synthesis methods.
Definition of submicron gels
In general, a gel is defined as a continuous colloid or polymeric network that fills an entire volume. A gel may be formed by covalent cross-linkages, physical interactions among its chains that could form aggregation, formation of glassy junctions by co-polymers, interlamellar interaction of polymers, and precipitation of flocculents in a polymeric network with a large structural deviation [13]. Submicron gels differ from the general description of the gel above, as they are a finite colloidal or polymeric network of which the pores are fully or semi-permeable to molecules of solvents with dimensions ranging from nano to micrometers. IUPAC in its “Gold Book” gave a straightforward definition of these gels according to size, wherein the size of the nano- and microgels were given as 1–100 nm and 0.1–100 μm, respectively [13]. The book also states that both nano and microgels could be gel particles of any shape from the above-mentioned size ranges. An alternative to this size-based definition can be found in [14], i.e. nanomaterials in general can be defined according to its applications—for biomedical applications, structures of up to 1000 nm may still be considered as nanostructured materials. Another definition of submicron gels is based on the occurrences of internal cross-linkages that covalently bind all the side chains in a single molecule [15]. This definition enables submicron gels to be classified as a new class of materials along with other types of macromolecules such as linear, branched, dendrimers, etc.
Classification of submicron gels
Gels, regardless of size and shape, natural or synthetic, can always be divided into two main groups. The first group consists of physical gels or pseudogels. This class of gel is formed via relatively weak physical bonds [16,17,18,19,20] such as Van der Waals, hydrogen bonding, hydrophilic/hydrophobic, or electrostatic interactions. Usually, gels in this group are formed via the self-assembly action of block co-polymers and/or graft co-polymers, which translates to weak binding forces. For example, increasing the applied stress, temperature, or even changing the pH or solvent would easily affect the physical structure of these gels.
The second group of gel consists of permanent gels. This type of gel is formed via relatively strong covalent bonds [21,22,23,24] that hold the shape of the gels. These permanent bonds give the gels the ability to sustain their shape under stress up until the degradation of the whole structure. This review will only focus on this second group of gels.
Generally, all types of submicron gels are referred to as submicron particles. A more specific classification apart from size range is therefore vital. Further details such as the morphology (capsules, sphere, rod, etc.), homogeneity/heterogeneity of the building monomer/polymer (polymer conjugates, etc.), and structure (layer-by-layer, core-shell particles) of the gels can also be taken into account. Depending on the factors mentioned above, submicron gels could be divided into classes, as show in Fig. 1.
Some examples of submicron gels are discussed in the following sections. The plain nanosphere is the most basic form of submicron gels. It is made up of a solid core of polymeric chains. The inner core can either be in the form of linear polymeric chains in a coiled position or a crosslinked polymeric network.
A nanocapsule resembles a submicron gel system with a thin polymeric skin encapsulating a reservoir or cargo. The outer layer of the nanocapsule consists of polymeric chains that are linked by physical forces or even chemical cross-linkages.
Hybrid submicron gels are based on a combination of polymeric gels and inorganic material. An example can found in the work of Zhu et al. [25] (Fig. 2). This type of gel has many advantages including stimuli-responsive properties based on volume phase transition.
Biohybrid nanogels are gels that consist of synthetic polymers and biomolecules such as microorganisms, enzymes, peptides, and biopolymers [26] as their building blocks. The synthetic part in hybrid molecules contributes towards their overall mechanical properties, stability in aqueous phase, and sites for incorporation of reactive groups for conjugation with dyes or other specific markers. On the other hand, the content of the biomolecules reflects their biological mechanism. These types of nanogels have unique properties and huge potential in terms of application that are not seen in other types of nanogels.
Materials variety
Submicron gels are considered engineering materials, and are thus expected to be superior over their macro-sized counterparts in terms of properties. They are designed to enhance the effectiveness of bioactive substances, therapeutic agents, imaging substances, etc., by improving their solubility, size, biodistribution, release kinetic, delivery, stability, and biocompatibility, among others, as well as providing a special passage across biological barriers. These properties are achieved by the introduction of a variety of materials according to the desired effects of the gels.
Drug solubilizing properties can be enhanced by altering the hydrophilicity or hydrophobicity ofsubmicron gels core material, depending on the inherent solubility of the drug itself. Theselection of certain molecular weight values enables the control of the size distribution of the gels, while drug loading and release properties can be carried out by material selection and/or the adjustment of crosslinking density. In the case of triggered-release delivery, submicron gels thatare sensitive towards stimuli such as temperature and pH can be designed by the incorporation ofmaterials such as PNIPAAM and PAA respectively. The introduction of hydrophilic materialsinto the building blocks of submicron gels may prolong circulation in blood, since hydrophilicmaterials such as PEG are known to cause a steric effect that prevents serum opsonins actionfrom reticuloendothelial system (RES). PEG also provides stealth properties for enhancedbiocompatibility[27]. Biodegradability properties, on the other hand, can be exerted by theincorporation of either synthetic or natural based polymers[28]. Lorenzo et al. reported thatnanogels with tailorable phase transition behavior can be obtained by controlling the amount ofits constituting block copolymers of various phase transition behaviors [29]. Kumar et al.demonstrated that the use of 4000 g/mol PEGDMA in PEGDMA/MAA microgels resulted in thelargest microgels with the highest insulin loading efficiency [30]. On the other hand, the samematerial used for the same nanogels may yield a significant difference in terms of result usingdifferent synthesis methods [31]. Table 1
lists the typical materials used to build submicron gels.
Applications of submicron gels
Submicron gels have wide-ranging applications in medical and industrial fields. In medical applications, they are designed according to intended therapeutic categories, target areas and administration modes. Design consideration includes various moieties that enabled attachment or conjugation of ligands for diagnostic, biomarker, molecular imaging, gene delivery, targeted delivery or drug. Like-wise, in industrial applications, they are usually used as support materials for synthesis of active inorganic materials that can be used as catalysts. Industrial applications include water purification i.e. arsenic removal from drinking water [32], oil spill recovery [33], ultra-hydrophobic coating [34]. Examples of submicron gels applications can be found in Table 2.
Size effect on different therapeutic areas
Important characteristics of nano/microgels include size, shape and surface properties. These factors are crucial for their in vivo biodistribution, since different therapeutic areas involve different barriers that present specific requirements during delivery. Size has a significant influence on gels circulation time in blood, biodistribution, tissue penetration and the fate of therapeutic agents. The widely accepted ideal size for nano/microgels in delivery applications is 70-200 nm. Particles larger than a micron will be opsonized, and results in accumulation in the liver and spleen, leading to a higher risk of aggregation. On the other hand, gel particles less than 5 nm are cleared rapidly by way of renal clearance and extravasation [79, 80]. Surface properties such as charge also have effect on renal clearance, where particles of the same size but with negative charge are not filtered through the glomerular basement membrane [81]. Additionally, a low positive charge value on particles may enhance cellular uptake, while at a higher value, toxicity issues may surface.
Therapeutic areas determine a certain practical size for active compound delivery. For example; lipophilic molecules (<600 Da) can penetrate into the skin through transdermal passive delivery [82]. Transdermal protein or peptide delivery such as insulin can be done using highly flexible vesicles such as transfersomes. Under non-occulated conditions and with hydration gradient, transfersomes, with a size of up to 200-300 nm can squeeze through skin pores that are one-tenth of the vesicles.
Tumors replicate faster compared to healthy tissues, and nutrients from the bloodstream are dominated by them as a result – potentially reducing healthy tissues to an inactive state. The rapid development of the tumor tissue hinders the nutrient supply to the inner layer of the tissue, leading to a build-up of necrotic core [83]. In order to continue growing beyond this point, the tumor stimulates rapid but poorly organized neovascularization [84] for nutrient supply. The newly-formed heterogenous blood vessels have high vascular permeability that allows the penetration of particles less than 100 nm through extravasation. On the other hand, a tumor also has poor lymphatic drainage which allows for the accumulation of anticancer drugs. The enhanced permeability and retention effect (EPR) of the tumor tissue enables nano/microparticles to be used as targeted drug delivery agents. However, therapy of the brain tumor is more complex due to the blood-brain barrier. The blood-brain barrier is formed by a tight endothelial layer that prevents passive accumulations of molecules in the brain. The pore size of the affected blood-brain barrier is around 12 nm, while it is smaller for the healthy tissue [8]. Particles 30-600 nm in size were tested with successes in animals, but only with the use of BBB permeating ligands [84].
Synthesis of submicron gels
Numerous methods to produce submicron gels have been presented in the literature. These gels can be produced via direct polymerization in homogenous or heterogeneous mixtures of different types of monomers, self-assembly mixtures, in an emulsion system, or in template-based nanofabrication such as Particle Replication in Nonwetting Templates (PRINT) or even polymerization involving systems with larger polymeric structures as their building blocks.
Normally, all types of production methods involve either grafting and/or crosslinking polymerization to form covalent bonds in the polymeric molecule backbone. In such processes, the substrate—be it a monomer or polymer—must contain at least one functional group that is polymerizable by the actions of radicals generated in the system.
Basically, radicals may be generated by either dissociation of ammonium persulfate in water [35, 85-87], degradation of chemical initiators such as N,N′-methylene-bis-acrylamide (classical chemistry) upon absorption of UV light photo initiator [87, 88] or radiolysis of water via ionizing radiation [78, 89, 90].
In the production of submicron gels, the polymerization process may take place in a solution (dilute solution polymerization) or sometimes involve the mechanism of emulsification (emulsion-based polymerization) to restrict the size of the final products as well as to avoid aggregation. Emulsification can be achieved using a certain type of surfactant depending on the design of the emulsion system.
Emulsion-based crosslinking polymerization
The synthesis of covalent-bonded submicron gels usually involves polymerization in a solution environment. In this case, the most important aspect of this process is to keep the polymerization in a confined space of which the size would depend on the size of the intended final product. Such confined spaces, which are also commonly known as micro or nanoreactors, are often formed using oil-in-water (direct) or water-in-oil (inverse) micelles.
Micelles—formed from the arrangement of surfactant in oil/water interphase—constitute micro or nanoreactors that are separated from each other [91]. Polymerization and crosslinking reactions in micro or nanoreactors normally leads to high monomer conversion [92, 93], making emulsion-based crosslinking polymerization a preferred technique in submicron gel production [94].
In a typical emulsion-based polymerization reaction (oil-in-water), radicals are generated from decomposition of the chemical initiators—also known as water radiolysis. These chemical initiators are normally generated outside of the micelles, where they react with monomers. The reacted monomers then transform into monomer radicals, and enter the emulsion via diffusion. The diffused monomer radicals would then react with other unreacted monomers. This will result in chain growth [15].
Emulsion-based polymerization may occur in both microemulsion and nanoemulsion. Although both types of emulsion are essentially prepared using the same materials (i.e. oil, water, and surfactant), the difference between the two lies in the thermodynamic stability [95] of both types of emulsions, which may render different effects on the final polymerization product. Further details regarding this issue will not be discussed in this review.
Direct micelles polymerization technique
Typically, emulsion polymerization processes involve several steps such as preparation of multilayer emulsion, and repeated dispersion or precipitation in certain sequences. These processes can sometimes be temperature and/or pH sensitive during synthesis. Unless justified, these techniques are normally avoided, as they are time consuming and expensive. Normally, a much simpler process, where a facile one-step polymerization reaction can be achieved, is always preferable.
Landfester et al. [34] studied varieties of miniemulsion techniques to synthesize polydimethylsiloxane (PDMS) nanogels. In their study, PDMS nanogels with sizes ranging from 40 to 1100 nm were obtained. They reported that the confinement provided by the small emulsions enabled more length scale copolymerization, thus highly crosslinked particles were obtained. The direct emulsion polymerization technique can also be used to synthesize magnetic-responsive nanogels from 4-vinylpyridine P (4-VP) [32]. Deen et al. [36] synthesized and characterized Poly (N-isopropylacrylamide) nanogels. They conducted an emulsion polymerization above the LCST and used a systematic influence of addition of surfactant on the final size and structure of the nanogels. Polymerization can also be initiated from the decomposition of chemical initiators using an argon laser exposure. In one study, amphiphilic nanoparticles were synthesized with a photo polymerization method [37]. In this study, direct micelles emulsion consisting of monomers, triethanolamine, and eosin Y were exposed to argon laser with a wavelength range of 480 to 520 nm for 1 h at room temperature. The DLS measurements showed that the nanoparticles produced ranged from 10 to 20 nm in size. Another study investigated the synthesis of dual-responsive nanogels using a novel initiator made from PEGylated AIBN [38]. The PEGylated AIBN was first synthesized using the method described in Walz et al. [96]. The product, PEG-AIBN-PEG, was then introduced to improve the macro-state and stability of the nanogels produced using this method.
Sometimes, direct micelles are preferred over inverse micelles for polymerization purposes due to the sensitivity of monomers towards water hydrolysis. One study synthesized nanogels from Poly(N-vinylcaprolactam) via polymerization using the direct micelles method [39]. First, the PVCL monomers, toluene, hexadecane, and AIBN were mixed with SDS in water. Polymerization was then carried out at 72 °C in 500-rpm stirring conditions, for 17 h. The study reports that the problems of monomer hydrolysis, low-solid content of the dispersions, and the nanogel re-dispersion in water were eliminated using this method. Natural-based submicron gels from deoxycholate (DE) and carboxymethyl (CM) chitosan were formed via the chemical crosslinking of emulsified chitosan solution in methylene dichloride. The final product was then studied using TEM, the size of which was determined to be 200–600 nm [97].
Direct micelles polymerization may also offer a clean technique for producing nanogels, whereby polymerization can be done in an aqueous medium with zero or small amounts of surfactant. An example of this method can be found in the synthesis of PVCL-based nanogels with ketal linkages [40] and poly-(oligo(ethylene glycol) methacrylate) (POEGMA)-based nanogels with tunable thermosensitivity properties [42].
Emulsion polymerization can also be combined with other techniques to achieve metabolic thiol-cleavable submicron gels. Nanogels from 6-O-vinyladipoyl-D-galactose (ODGal) were first synthesized via the enzymatic transesterification technique. These nanogels were then crosslinked with VCL and MAA via free radical emulsion polymerization [43]. More recent examples can be found in Sengel et al. [44] and Yi et al. [45]. Table 3
provides concise information pertaining to the selected methods discussed under this subsection. A typical polymerization process in direct micelles system is elucidated in Fig. 3
.
Inverse micelles polymerization technique
Normally, emulsion-based polymerization involves polymerization reactions of polymers in an aqueous matrix. This suits hydrophobic polymers well because in normal emulsion the surfactants that constitute micelles are arranged in such a way that the hydrophobic part of the surfactants are pointed inwards, away from the aqueous matrix. In this way, the confined spaces in the micelles would be hydrophobic and thus be more suitable for containing hydrophobic polymers. In cases where hydrophilic polymers are the reactants, the hydrophilic spaces to contain these polymers can then be achieved by inversing the arrangement of the surfactants. These types of micelles are known as inverse micelles (water-in-oil) (Fig. 4).
Inverse micelles polymerization has been proven to yield stable and uniform micron-sized polyacrylamide latexes of high molecular weight [98]. Candau et al. demonstrated that nanoparticles from a polyacrylamide latex can be synthesized using either exposure to thermal condition at 45 °C or UV irradiation at 25°C [33]. In both cases, free radicals were generated by decomposition of azobisisobutyronitrile (AIBN). It was reported that the monomer, acrylamide, acted as a co-surfactant that contributed to the stability of the inverse micelles. Additionally, the viscosity of the final product decreased due to a reduction in particle interactions.
Although PVCL is prone to water hydrolysis, being a hydrophilic polymer, the inverse micelle system is usually preferred for polymerization. In one study, biocompatible and thermally sensitive poly(N-vinylcaprolactam) nanogels were synthesized using an inverse microemulsion polymerization technique [41]. Natural-based polymers can also be polymerized in inverse micelle spaces to produce submicron gels. One example is the modification of hyaluronan acid (HA) [46]. HA microgels can also be obtained via attachment of aldehyde and hydrazide functionalities through oxidation with sodium periodate and coupling with glutahydrazide, respectively, in inverse micelles [70]. Another example of natural-based nanogels produced using the inverse micelles polymerization technique is a crosslinked star shaped acrylate arms with amine groups from various amino acids of a hen egg’s ovalbumin [47]. Another study also investigated the microspheres from chitosan that were produced by the introduction of covalent bonds among its amino group with the use of different crosslinking agents such as glutaraldehyde, sulphuric acid, and heat treatment [48].
One study used the above-mentioned method to synthesize nanogels with long-term stability and super low fouling ability against nonspecific protein adsorption from blood using poly(N-2-hydroxyethyl acrylamide) (polyHEAA) and acrylic acid (AA) [69]. As described in this work, the oil phase was prepared by mixing hexane, tween 80, Span 80, and ‘2,2’-azobis’(4-methoxy-2,4-dimethyl valeronitrile) (V-70), after which this mixture was kept on ice. The aqueous phase was prepared by dissolving HEAA, AA, and MBAA in 1 ml of DI water. Both the aqueous and oil phases were then added to a container and were shaken vigorously and sonocated for 2 min; the mixture was then purged with nitrogen. Polymerization was carried out at 40 °C for 4 h under nitrogen purging.
Inverse micelles polymerization is usually preferable against direct micelles polymerization, especially in the synthesis of submicron gels from polymers with good water solubility in both its monomer and polymer forms. In the case of polyacrylic acid (PAA), where both AA and PAA are soluble in the same polar medium during processing, formation of its submicron gels is impossible to control. Therefore, an inverse micelle system is a good alternative [49]. More recent works concerning this type of synthesis are described in Sahiner et al. [51], Su et al. [52], Hsiao et al. [53], Sun et al. [54] and Zhang et al. [55]. Table 4
provides a summary of the selected methods discussed under this subsection.
Surfactant-free micelles polymerization technique
Emulsion polymerization (Fig. 3) may come with several advantages, as discussed in the preceding section, but nevertheless, a few issues associated with utilization of surfactant are its main disadvantage. Surfactant and co-surfactant may sometimes become entrapped in the final product, thus it could be impossible to purify the final product from the bound surfactant molecules. However, as previously mentioned, emulsion-based polymerization can also be done in a surfactant-free system.
Surfactant-free emulsion polymerization has a few advantages in terms of submicron gel preparation. Clean and applicable surfaces can be readily obtained via this method [74]. Some examples can be found in a report by Singka et al. where pNIPAM nanogels were formed by grafting butyl acrylate (BA) [75] onto pNIPAM backbone and Zhou et al. where a POEGMA microgel was synthesized via the emulsifier free radical polymerization Fig. 5
technique [57].
Submicron gels can also be prepared using a chemical process that is known as heterogeneous free radical polymerization. This method normally involves monomers, chemical stabilizers, and chemical initiators that are soluble in its corresponding solvents. As the polymerization initializes, the resulting polymers will become insoluble in the solvent. As the polymers precipitate, the chemical stabilizers will stabilize the polymers by forming well-dispersed microgels. Some examples of this method can be seen in the preparation of PS [75], poly(methyl methacrylate) (PMMA) [76], and Poly(HFMA-co-NIPAM) [58] microgels.
Additionally, in one study, hydrophilic nanogels from NIPAM and VP were copolymerized in solution form without the use of a surfactant [59]. In another, hybrid nanogels containing lanthanum fluoride and copolymers were synthesized using the method of precipitation polymerization without surfactant [71]. The absence of surfactant in emulsion polymerization may cause emulsion instability, however, this issue can be overcome with the use of comonomers, as shown in a few researches [99, 100]. Comonomers can often act as a surfactant, as it normally has its own hydrophilic and hydrophobic parts. Hydrophilic comonomers normally surround the surface of the hydrophilic monomer, thus providing stability to the original emulsion.
Karabacak et al. [74] studied the effect of different types of monomers and pH value in the surfactant-free polymerization of PS colloids. In this study, 2-(dimethylamino)ethylmethacrylate (DMA) and methyl methacrylate (MMA) were found to be effective comonomers in a PS nanoparticle preparation via surfactant-free polymerization at different pH. The more hydrophilic the comonomers used in the process, the smaller the final particles produced.
A particular method of synthesis has been gaining popularity recently due to its minimalistic procedures. Known as the one-pot synthesis technique, this method has the advantage of simplicity. Additionally, the final product size can be easily tuned simply by varying the reactant concentration. In order to further overcome the need to use surfactants and any kind of stabilizer, Tang et al. [72] suggested a facile one-pot method in the preparation of nanogels through the combination of a step-growth polymerization with thermally-induced phase separation. The nanogel formation was initiated through the phase separation of thermo-sensitive polymers, where precursor particles formed. This was followed by aggregation and subsequent polymerization crosslinking of the precursor particles through an amine-epoxide reaction. Poly(propylene oxide) (PPO)-containing tri-amine (trade name Jeffamine T-403) and 1,3- butadiene bisepoxide were mixed at an amine to epoxide ratio of 1:0.95. The mixture was then incubated at 65 °C in a water bath for 15 min and rapidly cooled down to room temperature and diluted. The process was then repeated to grow the nanogels. The study reported that a higher monomer concentration would result in larger particles.
The one-pot facile method was used to synthesize nanogels with glucose-sensitive function [60]. Ramos et al. synthesized core-shell hybrid nanogels from N-vinylcaprolactam (VCL) with a 3-(trimethoxysilyl) propylmethacrylate (TPM) silica-based core [61]. Another study used the same method to synthesize HPMC/PAA hybrid nanogels in an aqueous-phase non-surfactant setup [62].
One study describes the synthesis of bioreducible and acid labile nanogels/microgels synthesis as “very simple” [101]. In the study, the nanogels were synthesized using this method without use of surfactants, stabilizers, or additives. However, the precursor materials were constructed using several pre-synthesis steps that in of itself had a yield value of 58–79%. pH-responsive Poly(vinyl-amine) (PVAM) microgels were synthesized in one other study using a two-step method based on a non-aqueous dispersion (NAD) polymerization method [63]. Nano or microgels can be used as a building block to form a nano-product. As an example, several researchers investigated the preparation of silver nanoparticles using an in situ reduction method of Ag + ions in NIPAM microgels [102]. Additionally, surfactant and organic solvents can both be avoided in this context. In a recent study, a pH-responsive alginate nanogel was synthesized using the one-pot technique without surfactant and organic solvent [103]. Recent works under this category can be found in Zhao et al.[64] and Abreu et al.[65]. Summary of selected methods under this subsection is given in Table 5.
Lithographic method
The advancement of submicron gels application in the field of modern diagnostics, advanced catalytic, treatment, etc. has initiated the development of vastly complex particles. These applications place great emphasis on the functionality of the candidate materials. On the other hand, hydrogels, in general, offer a great template for the development of designer chemicals such as smart structures that are able to react to stimuli such as pH, heat, electric potential, photon, etc. A technique known as lithography offers a high degree of control in engineering gels. Some examples of materials development using this method are briefly discussed in the following section.
One study involved the synthesis of a PEG micro ring using a soft lithographic method [73]. Briefly, PDMS was stamped with confined spaces in the order of picoliter and then put in contact with a flat PDMS film stained with a hexadexanethiol (HDT) ethanol solution. Next, a drop of PEGDA mixture with a photoinitator was applied to the stamp. The stamp was then pressed on a glass slide at 30 °C for 4 h followed by cooling to 4 °C. The PDMS stamp was lifted and the patterned substrate was annealed. The crosslinking process was initiated at 10 °C for 10 min using UV photo irradiation. A method that was developed based on the lithographic technique known, as Particle Replication in Nonwetting Templates (PRINT), as described in [66], was used to synthesize PEG microsized gels with variable sizes, shapes, and compositions.
One of the most recently explored areas in lithographic methodologies is the microfluidic technique. This technique affords a high degree of control in the design of intended colloids and narrow-size distributions. In this technique, a liquid monomer and its immiscible solvent reservoirs are channeled at an elevated pressure through specialized fabricated devices that consist of tapered micro channels with junctions, where the two liquids merge forming an emulsion and micro droplets. The crosslinking process can be done either by UV irradiation or polycondensation [67, 104].
Microgels with a pH-responsive character was developed using a microfluidic technique [68] for cell encapsulation and programmed release. Fabrication was done in 2 steps. First, cytocompatible dendritic polyglycerols (dPG) with acid-cleavable linkers were prepared. These macromonomers were then channeled through a microfluidic device together with a fibroblast cell (NIH3T3) from a mouse and homo-bifunctional PEG-dicylooctyne as the crosslinker. The crosslinking process in the emulsified mixture was then continued through bio-orthogonal strain-promoted azide–alkyne cycloaddition (SPAAC). The degradability of the final microgels could then be altered using dPG with different acid-cleavable linkers that would degrade with different kinetic conditions depending on the pH of the environment.
The microfluidic technique—while offering a high degree of control in the architecture of colloids and a narrow-sized distribution, as well as allowing for a surfactant and organic solvent-free process—also comes with a few limitations. If this method is to be employed for encapsulation of bioactive materials, the design of the whole procedure must be set so that the bioactive materials would not interact with the monomer, solvent, and other additives. Furthermore, this approach depends heavily on a high level of precision that is required in the fabrication of micro-moldings and other design aspects. Table 6
gives a summary on size and parameters on methods discussed under this subsection.
Irradiation method
Staudinger et al. first reported the application of an irradiation-induced method in submicron gels in the 1930s, where styrene divinylbenzene microgels were formed upon exposure to irradiation. Subsequently, more and more works have since been reported, covering a multitude of different methods and microgels from different starting materials. The irradiation-induced method has since become more popular due to its additive-free initiation, ability of crosslinking/grafting, particle size control, and its functionalization and sterilization in synthesizing submicron gels, which can be achieved in just one step.
Generally, radiation-induced synthesis of submicron gels can be classified into two groups, and this depends on the selection of the reactant that will influence the dominant crosslinking process that ensues during the recombination of radicals. The first group is characterized by inter-molecular crosslinking, often involving reactants from the monomer or its mixtures. The second group emphasizes on the intra-molecular crosslinking of one single polymer [15, 105].
Submicron gels are formed when a monomeric/polymeric solution or emulsion is exposed to ionizing irradiation. The radiation energy is mostly absorbed by the water component up to a certain energy threshold; at which point the water molecules will decompose into several short-lived radicals. Among these, the hydroxyl radicals and hydrogen atoms will be the two radicals with the potential to convert a monomer or polymer into microradicals i.e. via hydrogen abstraction. The recombination of two separate microradicals will lead to intermolecular crosslinking, normally accompanied with an increase in molecular weight and the eventual formation of a large single macroscopic gel due to an exceedingly high degree of crosslinking. On the other hand, recombination of radicals from the same chain is known as intra-molecular cross-linking, where separate micromolecules in nano and micro scales can be obtained. Recombination of radicals under this condition does not affect the molecular weight of the resulting gel. Several examples of ionizing radiation-induced syntheses of submicron gels are discussed in the following section.
Ulanski et al. synthesized PAA nanogels by irradiating PAA at a pH of 2.0 in a closed-loop system with continuous flow of PAA. They reported that at a higher pH, chain scission is more likely to occur due to the repulsive forces between negatively charged polyelectrolyte chains. In order to promote crosslinking, the pH of the polyelectrolyte solution was adjusted to 2.0 to neutralize the repulsive forces that prevent adjacent polymers to merge. Furthermore, to maximize intramolecular crosslinking, the synthesis was done by irradiating a dilute solution of PAA with a high dose rate of electron beams in pulse mode. The average molecular weight, intrinsic viscosities, and radius of gyration data suggest that intramolecular crosslinking has taken place and nanogels have been formed [50]. The same group of researchers also synthesized PVP nanogels by irradiating a deoxygenated dilute aqueous solution of the polymer with a very high dose rate of electrons in pulse mode. As PVP does not contain any functional group that is sensitive towards solvated electrons, the sample was thus saturated with nitrous oxide to convert the solvated electron into hydroxyl radicals. As proven by the researchers, the PVP reacted with hydroxyl radicals by means of hydrogen abstraction. They reported that there was no change in molecular weight, but the size of the gel decreased, thus proving the formation of nanogels and intramolecular crosslinking [89].
Henke et al. synthesized Polyvinylpyrrolidone (PVP) and PAA nanogels in their study. They first prepared PVP and PAA complexes to obtain a certain molar fraction of carboxylic groups. The mixture was then irradiated in a glass vessel under fast stirring with electrons in pulses [106]. The PVP and PAA mixture was kept at a low concentration to avoid intermolecular crosslinking. They reported that there was a small increase in molecular weight for the irradiated mixture in comparison to the irradiated pure PVP. The small increase in molecular weight was the result of some intermolecular crosslinking that occurred simultaneously with the dominant intramolecular crosslinking processes. The intrinsic viscosity and radius of gyration of the microgels decreased at higher doses, suggesting evidence of formation of tightly crosslinked nanogels.
PVP nanogels were synthesized by intra molecular crosslinking via pulse radiolysis. PVP solutions were prepared by mixing PVP monomer, NaCl, and KCl in a buffer solution at a pH of 7.4 [107]. PVP can also be grafted to AA using high-energy electron beam irradiation to synthesize nanogels. They reported that nanogels with controlled size distribution and functionality was achieved using this method [78,108]. Hybrid nanogels can also be synthesized using an irradiation-induced method. For example, in one study, temperature-responsive nanogels were prepared via the immobilization of quantum dots in the interior spaces of nanogels [25].
Apart from chemical initiators, emulsion polymerization can also be initiated using ionizing radiation. Song et al. demonstrated that nanogels could be produced via the irradiation of styrene microemulsion. The size of the polystyrene (PS) gel obtained ranged from 52 to 210 nm [109]. The same application of ionizing radiation can also be used on inverse micelle systems, as reported by Yusof et al. [22] in their synthesis of PEGDA nanogels. In their work, a radiation crosslinkable polymer PEGDA solution, n heptane, and dioctyl sulfosuccinate (AOT) were first mixed. Then, the mixture was stirred until a clear one-phase solution formed and then exposed to an electron beam. The emulsion proved to be a suitable template for the polymerization of water-soluble crosslinkable polymers such as PEGDA (Fig. 6 a). Upon irradiation of a ternary system, the energy imparted will be absorbed by the oil phase and scavenged by the micelles to produce a hydroxyl radical via the direct radiolysis of water (b). Polymeric macroradicals are then formed due to hydroxyl radical attack (c). Macroradicals that are generated inside the micelle then recombine to form nanogels in the micelle (d).
Several works involving surfactant-free irradiation methods have been presented in the past including [50, 77, 89, 110, 111]. All these works have a disadvantage in terms of controlling the molecular weight and size of the final product. In 2012, Kadlubowski et al. suggested a new method that would allow for the control of the size and molecular weight of a gel [112]. In this new method, nanogels from PVP were synthesized in two stages (Fig. 7).
In the first stage, a PVP solution was prepared to a concentration above its critical hydrodynamic concentration. The solution was then irradiated at a low dose rate to yield PVP microradicals at 10−7 mol dm−3 of concentration, in a system with a much higher amount of polymer coils than the microradical concentration value with the amount of average radicals per macromolecule being lower than 1. Under this condition, the macromolecules in the system would prefer intermolecular crosslinking, which would lead to an increase in size and molecular weight. The second stage of the process was done to stop further growth of the macromolecules. The above-mentioned irradiated solution was then diluted to a concentration lower than its coil overlapping concentration and then subjected to pulses of fast electron. Short and intense pulses of fast electrons generated radical concentrations of around 10−4–10−3 mol dm−3. Such a high value in a diluted macromolecules solution may result in the simultaneous creation of many radicals on each of the micromolecule. Under this condition, intramolecular crosslinking is preferable, at which point nanogels with almost constant molecular weight are formed regardless of dose increment. By adjusting the initial dose during gamma irradiation followed with dilution and exposure to pulse irradiation, nanogels with certain molecular weight and size can readily be obtained (Fig. 8).
Irradiation-induced synthesis can also be used to synthesize nanoparticles from natural polymers. Although the normal response of natural polymers towards ionizing irradiation is fragmentation and aggregation, under certain processing conditions, crosslinking to form nanoparticles can be achieved. In one study, protein nanogels were prepared via the gamma irradiation of bovine serum albumin (BSA) as the basic material [23, 113, 114]. The BSA was dissolved in ethanol solution, and then irradiated with gamma rays at a low dose rate at a relatively low temperature range. It was found that different concentrations of ethanol in the albumin solution led to different-sized nanogels.
The radiation-induced synthesis of nanogels can also be combined with other controlled-radical polymerization such as the Reversible Addition–Fragmentation Chain Transfer (RAFT) method to achieve narrow molecular weight distributions, controlled molecular weights, and complex architectures. Recently, the PNIPAAM nanogel was prepared using two RAFT agents in dimethylformamide (DMF). The polymerization was initiated using gamma rays [115]. Amphiphilic copolymers containing temperature and pH sensitive units with hydrophobic cores were produced using the same combination of methods [29].
Apart from being able to produce submicron gels from reactants in solution form, radiation-induced polymerization is also useful in the preparation of microgels from bulk materials. In a Brabender mixing chamber coupled with an electron beam setup, thermoplastic microgels from polypropylene (PP) and ethylene octene copolymer (EOC) were prepared via electron-induced reactive processing. Briefly, equal amounts of PP and EOC were melted and mixed in a mixing chamber at a speed of 45 rpm and at 180 °C in the presence of air. The mixing process was accompanied with simultaneous exposure to electron beams at 100 kGy with electron energy of 1.5 MeV. The microgels were used as an additive material in a thermoplastic matrix to improve elasticity and melt processability [21]. Recent report related to this type of synthesis method can be found in the work of Cruz et al. [116]. Summary of selected methods discussed under this subsection can be found in Table 7.
The above review indicates that the irradiation route is obviously an attractive method in producing submicron gels, as the process normally comprises a single-step technique. Also, in some cases, the doses applied are equivalent to a sterilization dose. Therefore, in addition to polymerization, the irradiated samples are also sterilized. Nevertheless, there is one disadvantage to this method—although it is facility-dependent, the availability of ionizing irradiators is still low and access to similar services is also limited. Table 8
gives a summary in terms of methods, materials, advantages and disadvantages of methods from different categories discussed in this review.
Conclusion
This review presents an overview of the basic aspects regarding the definition, classification, and synthesis methods of organic submicron gels. Submicron gels have undergone interesting transformations from being an unwanted by-product to now being applied in many emerging advanced applications. The emergence of new applications, meanwhile, has driven the discovery of new technique of syntheses that allow for precise tailoring of the final products. Submicron gels can be synthesized using emulsion-based crosslinking, lithographic methods, and irradiation-induced methods—each with its own strengths, advantages, and disadvantages. The selection of methods can sometimes be limited to the properties of the reactant (i.e. water solubility, economic, application, facility, etc.). Nevertheless, these methods can also be combined to complement each other, resulting in a far superior process. In summary, one would find that the best combination of processing techniques would comprise a one-pot aqueous setup coupled with irradiation-induced polymerization/crosslinking on a polymer solution as the starting material. This setup would allow for the highly advantageous processing of submicron gels.
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