Abstract
The effects of low counts on assemblage inferences in paleolimnological investigations have been examined for many biological proxies, but not yet for Cladocera. Established guidelines leading to the determination of an adequate, minimum count are absent with respect to sampling cladoceran remains from lake sediments. Using simulated subsamples derived from observed assemblages of considerably higher counts, we investigated the effect of counting effort on three principal characteristics of an assemblage: richness, number of new taxa encountered, and the absolute differences in relative abundances of dominant taxa. Results from six lakes located within diverse ecological regions (i.e. Subarctic, Canadian Shield, Acadian Forest) suggest that a minimum of between 70 and 100 individuals is satisfactory to characterize most assemblages. Doubling counting effort from 100 to 200 individuals leads to only modest gains in subsample relatedness to the observed assemblage. Greater counting effort may be necessary when the primary interest is in presence-absence or distributional data, or when abundances of ecologically relevant taxa are exceptionally low.
Similar content being viewed by others
References
Battarbee RW (1986) Diatom analysis. In: Berglund BE (ed) Handbook of Holocene palaeoecology and palaeohydrology. Wiley, Chichester, pp 527–570
Birks HJB (1998) Numerical tools in palaeolimnology—progress, potentialities, and problems. J Paleolimnol 20:307–332
Birks HJB, Line JM (1992) The use of rarefaction analysis for estimating palynological richness from Quaternary pollen analytical data. Holocene 2:1–10
Bodén P (1991) Reproducibility in the random settling method for quantitative diatom analysis. Micropaleontology 37:313–319
Bos DG (2001) Sedimentary cladoceran remains, a key to interpreting past changes in nutrient and trophic interactions. PhD Thesis, Queen’s University, Kingston
Brodersen KP, Whiteside MC, Lindegaard C (1998) Reconstruction of trophic state in Danish lakes using subfossil chydorid (Cladocera) assemblages. Can J Fish Aquat Sci 55:1093–1103
Deevey ES (1964) Preliminary account of fossilization of zooplankton in Rogers Lake. Verh Int Ver Limnol 115:981–992
DeSellas AM, Paterson AM, Sweetman JN, Smol JP (2008) Cladocera assemblages from the surface sediments of south-central Ontario lakes and their relationships to measured environmental variables. Hydrobiologia 600:105–119
Finney BP, Gregory-Eaves I, Sweetman J, Douglas MSV, Smol JP (2000) Impacts of climatic change and fishing on Pacific salmon abundance over the past 300 years. Science 290:795–799
Frey DG (1960) The ecological significance of cladoceran remains in lake sediments. Ecology 41:684–699
Frey DG (1982) The reticulated species of Chydorus (Cladocera, Chydoridae): two new species with suggestion of convergence. Hydrobiologia 93:255–279
Frey DG (1986) Cladocera analysis. In: Berglund BE (ed) Handbook of palaeoecology and palaeohydrology. Wiley, New York, pp 667–692
Frey DG (1987) The taxonomy and biogeography of the Cladocera. Hydrobiologia 145:5–17
Frey DG (1988) Littoral and offshore communities of diatoms, cladocerans, and dipterous larvae, and their interpretation in paleolimnology. J Paleolimnol 1:179–191
Ginn BK, Cumming BF, Smol JP (2007) Assessing pH changes since pre-industrial times in 51 low-alkalinity lakes in Nova Scotia, Canada. Can J Fish Aquat Sci 64:1043–1054
Glew J (1988) A portable extruding device for close interval sectioning of unconsolidated core samples. J Paleolimnol 1:235–239
Glew J (1991) Miniature gravity corer for recovering short sediment cores. J Paleolimnol 5:285–287
Gregory-Eaves I, Smol JP, Finney BP, Lean DRS, Edwards ME (2000) Characteristics and variation in lakes along a north-south transect in Alaska. Arch Hydrobiol 147:193–223
Hann BJ (1989) Cladocera. Methods in Quaternary ecology. Geosci Can 16:17–26
Heiri O, Lotter AF (2001) Effect of low count sums on quantitative environmental reconstructions: an example using subfossil chironomids. J Paleolimnol 26:343–350
Hofmann W (1998) Cladocerans and chironomids as indicators of lake-level change in north temperate lakes. J Paleolimnol 19:55–62
Hofmann W (2000) Response of chydorid faunas to rapid climatic changes in four alpine lakes at different altitudes. Palaeogeogr Palaeoclimatol Palaeoecol 159:281–292
Hudson JJ, Taylor WD, Schindler DW (1999) Planktonic nutrient regeneration and cycling efficiency in temperate lakes. Nature 400:659–661
Järvinen M, Salonen K (1998) Influence of changing food web structure on nutrient limitation of phytoplankton in a highly humic lake. Can J Fish Aquat Sci 55:2562–2571
Jeppesen E, Leavitt P, De Meester L, Jensen JP (2001a) Functional ecology and palaeolimnology: using cladoceran remains to reconstruct anthropogenic impact. Trends Ecol Evol 16:191–198
Jeppesen E, Jensen JP, Skovgaard H, Hvidt CV (2001b) Changes in the abundance of planktivorous fish in Lake Skanderborg during the past two centuries—a palaeoecological approach. Palaeogeogr Palaeoclimatol Palaeoecol 172:143–152
Jeziorski A, Yan ND, Paterson AM, DeSellas AM, Turner MA, Jeffries DS, Keller B, Weeber RC, McNicol DK, Palmer ME, McIver K, Arseneau K, Ginn BK, Cumming BF, Smol JP (2008) The widespread threat of calcium decline in fresh waters. Science 322:1374–1377
Kattel GR, Battarbee RW, Mackay A, Birks HJB (2007) Are cladoceran fossils in lake sediment samples a biased reflection of the communities from which they are derived? J Paleolimnol 38:157–181
Kerfoot WC (1981) Long-term replacement cycles in cladoceran communities: a history of predation. Ecology 62:216–233
Kerfoot WC, Robbins JA, Weider LJ (1999) A new approach to historical reconstruction: combining descriptive and experimental paleolimnology. Limnol Oceanogr 44:1232–1247
Korhola A, Rautio M (2001) Cladocera and other brachiopod crustaceans. In: Smol JP, Birks HJB, Last WM (eds) Tracking environmental change using lake sediments: zoological indicators. Kluwer, Dordrecht, pp 5–41
Korhola A, Tikkanen M, Weckström J (2005) Quantification of Holocene lake-level changes in Finnish Lapland using a cladoceran-lake depth transfer model. J Paleolimnol 34:175–190
Korosi JB, Paterson AM, DeSellas AM, Smol JP (2008) Linking mean body size of pelagic Cladocera to environmental variables in Precambrian Shield lakes: a paleolimnological approach. J Limnol 67:22–34
Larocque I (2001) How many chironomid head capsules are enough? A statistical approach to determine sample size for palaeoclimatic reconstructions. Palaeogeogr Palaeoclimatol Palaeoecol 172:133–142
Lotter AF, Birks HJB, Eicher U, Hofmann W, Schwander J, Wick L (2000) Younger Dryas and Allerød summer temperatures at Gerzensee (Switzerland) inferred from fossil pollen and cladoceran assemblages. Palaeogeogr Palaeoclimatol Palaeoecol 159:349–361
Lytle DE, Wahl ER (2005) Palaeoenvironmental reconstructions using the modern analogue technique: the effects of sample size and decision rules. Holocene 15:554–566
Nykänen M, Vakkilainen K, Liukkonen M, Kairesalo T (2009) Cladoceran remains in lake sediments: a comparison between plankton counts and sediment records. J Paleolimnol 42:551–570
Paterson MJ (1994) Paleolimnological reconstruction of recent changes in assemblages of Cladocera from acidified lakes in the Adirondack Mountains (New York). J Paleolimnol 11:189–200
Payne RJ, Mitchell EAD (2009) How many is enough? Determining optimal count totals for ecological and palaeoecological studies of testate amoebae. J Paleolimnol 42:483–495
Quinlan R, Smol JP (2001) Setting minimum head capsule abundance and taxa deletion criteria in chironomid-based inference models. J Paleolimnol 26:327–342
Rautio M, Sorvari S, Korhola A (2000) Diatom and crustacean zooplankton communities, their seasonal variability and representativeness in the sediment of subarctic Lake Saanajärvi. J Limnol 59:81–96
Sarmaja-Korhonen K, Kultti S, Solovieva N, Valiranta M (2003) Mid-Holocene palaeoclimatic and palaeohydrological conditions in northeastern European Russia: a multi-proxy study of Lake Vankavad. J Paleolimnol 30:415–426
Seber GAF, Wild CJ (1989) Nonlinear regression. Wiley, New York
Smirnov NN (1974) Fauna of the U.S.S.R., Crustacea. Vol 1: Chydoridae. Israel Program for Scientific Translations, Jerusalem
Smirnov NN (1996) Cladocera: the Chydorinae and Sayciinae (Chydoridae) of the World. SPB Academic Publishing, Amsterdam
Sweetman JN, Finney BP (2003) Differential responses of zooplankton populations (Bosmina longirostris) to fish predation and nutrient loading in an introduced and natural sockeye salmon nursery lake on Kodiak Island, Alaska, USA. J Paleolimnol 30:183–193
Sweetman JN, Smol JP (2006a) Patterns in the distribution of cladocerans (Crustacea: Branchiopoda) in lakes across a north-south transect in Alaska, USA. Hydrobiologia 553:277–291
Sweetman JN, Smol JP (2006b) A guide to the identification of cladoceran remains (Crustacea, Branchiopoda) in Alaskan lake sediments. Arch Hydrobiol Suppl 151:353–394
Sweetman JN, LaFace E, Rühland KM, Smol JP (2008) Evaluating the response of Cladocera to recent environmental change in lakes from the Canadian Arctic treeline region. Arct Antarct Alp Res 40:584–591
Szeroczyńska K, Sarmaja-Korjonen K (2007) Atlas of subfossil Cladocera from central and northern Europe. Friends of the Lower Vistula Society, Świecie
Taylor DJ, Ishikane CR, Haney RA (2002) The systematics of Holarctic Bosminids and a revision that reconciles molecular and morphological evolution. Limnol Oceanogr 47:1486–1495
ter Braak CFJ, Juggins S (1993) Weighted averaging partial least squares regression (WA-PLS): an improved method for reconstructing environmental variables from species assemblages. Hydrobiologia 269–270:485–502
Toms JD, Lesperance ML (2003) Piecewise regression: a tool for identifying ecological thresholds. Ecology 84:2034–2041
Urabe J, Elser JJ, Kyle M, Yoshida T, Sekino T, Kawabata Z (2002) Herbivorous animals can mitigate unfavourable ratios of energy and material supplies by enhancing nutrient recycling. Ecol Lett 5:177–185
Wall AAJ, Gilbert D, Magny M, Mitchell EAD (2010) Testate amoeba analysis of lake sediments: impact of filter size and total count on estimates of density, species richness and assemblage structure. J Paleolimnol 43:689–704
Witty LM (2004) Practical guide to identifying freshwater crustacean zooplankton. Cooperative Freshwater Ecology Unit. Laurentian University, Sudbury
Acknowledgments
Funding for this research was provided by a Natural Sciences and Engineering Research Council of Canada (NSERC) grant to JPS and a NSERC Postdoctoral Fellowship to JK. We also thank Brendan Wiltse and Heather Haig for assistance implementing the R code. Two anonymous reviewers and Oliver Heiri (Associate Editor) also provided useful comments and suggestions.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kurek, J., Korosi, J.B., Jeziorski, A. et al. Establishing reliable minimum count sizes for cladoceran subfossils sampled from lake sediments. J Paleolimnol 44, 603–612 (2010). https://doi.org/10.1007/s10933-010-9440-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10933-010-9440-6