Abstract
This study compares the marker pigment composition in three different lake compartments: the water column; the surface sediment biofilm (0–0.5 cm), where production and main degradation processes take place; and the deep sediment (15–17 cm), where the signal is finally stored and marker pigments are used as a proxy in paleolimnological studies. The aim was to evaluate which marker pigments better record in the sediment the relative contributions of planktonic and benthic production in high mountain lakes, taking into account the differential preservation existing among pigments. A survey of 82 high mountain lakes distributed along the major environmental gradients was carried out in the Pyrenees. Comparison of pigments between the three compartments revealed that both water column and sediment biofilm signals could be distinguished in old sediment layers, despite the observation that some of the most characteristic carotenoids that appeared frequently and in high concentrations in the water column were already less common or even absent from the sediment biofilm. In the shallowest lakes, pigment composition in the sediment biofilm was typical of photosynthetically active communities and their deep sediment samples were characterised by a substitution of fucoxanthin by diatoxanthin as the dominant diatom marker pigment. However, in the deepest lakes the pigment composition of the sediment biofilm and deep sediment was similar, characterised by marker pigments of mainly planktonic algal groups and pheophytins, which are typical pigments of decaying communities. Results are discussed in terms of how pigment source (planktonic or benthic) and pigment type (lability of molecule) interact to shape pigment composition in the sediment record of high mountain lakes.
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Battarbee RW, Grytnes JA, Thompson R, Appleby P, Catalan J, Korhola A, Birks HJB, Heegaard E, Lami A (2002) Comparing palaeolimnological and instrumental evidence of climate change for remote mountain lakes over the last 200 years. J Paleolimnol 28:161–179
Bonilla S, Villeneuve V, Vincent WF (2005) Benthic and planktonic algal communities in a High Arctic Lake: pigment structure and contrasting responses to nutrient enrichment. J Phycol 41:1120–1130
Borrego CM, Arellano JB, Abella CA, Gillbro T, Garcia-Gil J (1999) The molar extinction coefficient of bacteriochlorophyll e and the pigment stoichiometry in Chlorobium phaeobacteroides. Photosynth Res 60:257–264
Britton G, Liaaen-Jensen S, Pfander H (1995) Carotenoids: volume 1A: isolation and analysis. Birkhäuser Verlag, Boston
Buchaca T, Catalan J (2007) Factors influencing the variability of pigments in the surface sediments of mountain lakes. Freshwater Biol 52:1365–1379
Buchaca T, Felip M, Catalan J (2005) A comparison of HPLC pigment analyses and biovolume estimates of phytoplankton groups in an oligotrophic lake. J Plankton Res 27:91–101
Cameron NG, Birks HJB, Jones VJ, Berge F, Catalan J, Flower RJ, García J, Kawecka B, Koining KA, Marchetto A, Sánchez Castillo P, Schmidt R, Sisko M, Solovieva N, Stefkova E, Toro M (1999) Surface-sediment and epilithic diatom pH calibration sets for remote European lakes (AL:PE project) and their comparison with the Surface Waters Acidification Programme (SWAP) calibration set. J Paleolimnol 22:291–317
Carpenter SR, Elser MM, Elser JJ (1986) Chlorophyll production, degradation and sedimentation: implications for paleolimnology. Limnol Oceanogr 31:112–124
Catalan J (1992) Evolution of dissolved and particulate matter during the ice-covered period in a deep, high-mountain lake. Can J Fish Aquat Sci 49:945–955
Catalan J, Ballesteros E, Gacia E, Palau A, Camarero L (1993) Chemical composition of disturbed and undisturbed high-mountain lakes in the Pyrenees: a reference for acidified sites. Water Res 27:133–141
Catalan J, Ventura M, Brancelj A, Granados I, Thies H, Nickus U, Korhola A, Lotter AF, Barbieri A, Stuchlík E, Lien L, Bitušík P, Buchaca T, Camarero L, Goudsmit GH, Kopácek J, Lemcke G, Livingstone DM, Müller B, Rautio M, Šiško M, Sorvari S, Šporka F, Strunecký O, Toro M (2002) Seasonal ecosystem variability in remote mountain lakes: implications for detecting climatic signals in sediment records. J Paleolimnol 28:25–46
Cuddington K, Leavitt PR (1999) An individual-based model of pigment flux in lakes: implications for organic biogeochemistry and paleoecology. Can J Fish Aquat Sci 56:1964–1977
Daley RJ (1973) Experimental characterization of lacustrine chlorophyll diagenesis. II. Bacterial, viral and herbivore grazing effects. Arch Hydrobiol 72:409–439
Daley RJ, Brown SR (1973) Experimental characterization of lacustrine chlorophyll diagenesis. I. Physiological and environmental effects. Arch Hydrobiol 72:277–304
Davies BH (1976) Carotenoids. Academic Press, London
Dreßler M, Hübener T, Görs S, Werner P, Selig U (2007) Multi-proxy reconstruction of trophic state, hypolimnetic anoxia and phototrophic sulphur bacteria abundance in a dimictic lake in Northern Germany over the past 80 years. J Paleolimnol 37:205–219
Felip M, Catalan J (2000) The relationship between phytoplankton biovolume and chlorophyll in a deep oligotrophic lake: decoupling in their spatial and temporal maxima. J Plankton Res 22:91–105
Fietz S, Nicklisch A, Oberhänsli H (2007) Phytoplankton response to climate changes in lake Baikal during the Holocene and Kazantsevo Interglacials assessed from sedimentary pigments. J Paleolimnol 37:177–203
Glew J (1991) Miniature gravity corer for recovering short sediment cores. J Paleolimnol 5:285–287
Härdle W (1991) Smoothing techniques with implementation in S. John Wiley and Sons, New York
Heiri O, Lotter AF, Lemcke G (2001) Loss on ignition as a method for estimating organic and carbonate content in sediments: reproducibility and comparability of results. J Paleolimnol 25:101–110
Hodgson DA, Vyverman W, Verleyen E, Sabbe K, Leavitt PR, Taton A, Squier AH, Keely BJ (2004) Environmental factors influencing the pigment composition of in situ benthic microbial communities in east Antarctic lakes. Aquat Microb Ecol 37:247–263
Hodgson DA, Wright SW, Tyler PA, Davies N (1998) Analysis of fossil pigments from algae and bacteria in meromictic Lake Fidler, Tasmania, and its application to lake management. J Paleolimnol 19:1–22
Hurley JP, Armstrong DE (1990) Fluxes and transformations of aquatic pigments in Lake Mendota, Wisconsin. Limnol Oceanogr 35:384–398
Hurley JP, Armstrong DE (1991) Pigment preservation in lake sediments: a comparison of sedimentary environments in Trout Lake, Wisconsin. Can J Fish Aquat Sci 48:472–486
Jeffrey SW, Hallegraeff GM (1987) Chlorophyllase distribution in ten classes of phytoplankton: a problem for chlorophyll analysis. Mar Ecol Prog Ser 35:293–304
Jeffrey SW, Mantoura RFC, Wright SW (1997) Phytoplankton pigments in oceanography: guidelines to modern methods. UNESCO Publishing, Paris
Kraay GW, Zapata M, Veldhuis MJW (1992) Separation of chlorophylls c1, c2 and c3 of marine phytoplankton by reversed-phase C18 high-performance liquid chromatography. J Phycol 28:708–712
Leavitt PR (1993) A review of factors that regulate carotenoid and chlorophyll deposition and fossil pigment abundance. J Paleolimnol 9:109–127
Leavitt PR, Brown SR (1988) Effects of grazing by Daphnia on algal carotenoids: implications for paleolimnology. J Paleolimnol 1:201–213
Leavitt PR, Carpenter SR (1989) Effects of sediment mixing and benthic algal production on fossil pigment stratigraphies. J Paleolimnol 2:147–158
Leavitt PR, Carpenter SR (1990) Aphotic pigment degradation in the hypolimnion: implications for sedimentation studies and paleolimnology. Limnol Oceanogr 35:520–534
Leavitt PR, Hodgson DA (2001) Sedimentary pigments. In: Smol JP, Birks HJB, Last WM (eds) Terrestrial, algal, and siliceous indicators. Kluwer Academic Publishers, Dordrecht, pp 295–325
Lee RE (1999) Phycology. Cambridge University Press, Cambridge
Liaaen-Jensen S (1965) Bacterial carotenoids. Acta Chem Scand 19:1025–1030
Louda JW, Liu L, Baker EW (2002) Senescence- and death-related alteration of chlorophylls and carotenoids in marine phytoplankton. Org Geochem 33:1635–1653
Mallorquí N, Arellano JB, Borrego CM, Garcia-Gil JL (2005) Signature pigments of green sulfur bacteria in lower Pleistocene deposits from the Banyoles lacustrine area (Spain). J Paleolimnol 34:271–280
Mantoura RFC, Llewellyn CA (1983) The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography. Anal Chim Acta 151:297–314
Margalef R (1983) Limnología. Omega, Barcelona
Moss B (1968) Studies on the degradation of chlorophyll a and carotenoids in freshwaters. New Phytol 67:49–59
Proteau PJ, Gerwick WH, Garcia-Pichel F, Castenholz R (1993) The structure of scytonemin, an ultraviolet sunscreen pigment from the sheaths of cyanobacteria. Experientia 49:825–829
Repeta DJ, Gagosian RB (1984) Transformation reactions and recycling of carotenoids and chlorins in the Peru upwelling region (15° S, 75° W). Geochim Cosmochim Acta 46:1265–1277
Rowan KS (1989) Photosynthetic pigments of algae. Cambridge University Press
Sanger JE (1988) Fossil pigments in paleoecology and paleolimnology. Palaeogeogr Palaeoclimat Palaeoecol 62:343–359
Sanger JE, Gorham E (1970) The diversity of pigments in lake sediments and its ecological significance. Limnol Oceanogr 15:59–69
Sanger JE, Gorham E (1972) Stratigraphy of fossil pigments as a guide to the postglacial history of Kirchner Marsh, Minnesota. Limnol Oceanogr 17:840–854
Scheer H (1991) Structure and occurrence of chlorophylls. In: Scheer H (ed) Chlorophylls. CRC Press, Boca Raton, Florida
Sinninghe Damsté JS, Koopmans MP (1997) The fate of carotenoids in sediments: an overview. Pure Appl Chem 69/10:2067–2074
Soma Y, Tani Y, Soma M, Mitake H, Kurihara R, Hashomoto S, Watanabe T, Nakamura T (2007) Sedimentary steryl chlorin esters (SCEs) and other photosynthetic pigments as indicators of paleolimnological change over the last 28,000 years from the Buguldeika Saddle of Lake Baikal. J Paleolimnol 37:163–175
Steenbergen CLM, Korthals HJ, Dobrynin EG (1994) Algal and bacterial pigments in non-laminated lacustrine sediment: studies of their sedimentation, degradation and stratigraphy. FEMS Microbiol Ecol 13:335–351
Steinman AD, Havens KE, Louda JW, Winfree NM, Baker EW (1998) Characterization of the photoautotrophic algal and bacterial communities in a large, shallow, subtropical lake using HPLC-PDA based pigment analysis. Can J Fish Aquat Sci 55:206–219
Swain EB (1985) Measurement and interpretation of sedimentary pigments. Freshwater Biol 15:53–75
Ter Braak CJF (1994) Canonical community ordination. Part I: basic theory and linear methods. Ecoscience 1:127–140
Ter Braak CJF, Šmilauer P (2002) CANOCO Reference manual and CanoDraw for Windows user’s guide: software for canonical community ordination. (4.5). Microcomputer Power, Ithaca, NY
Ventura M, Camarero L, Buchaca T, Bartumeus F, Livingstone DM, Catalan J (2000) The main features of seasonal variability in the external forcing and dynamics of a deep mountain lake (Redó, Pyrenees). J Limnol 59:97–108
Villanueva J, Grimalt JO, De Wit R, Keely BJ, Maxwell JR (1994) Sources and transformations of chlorophylls and carotenoids in a monomictic sulphate-rich karstic lake environment. Org Geochem 22:739–757
Villanueva J, Hastings DW (2000) A century-scale record of the preservation of chlorophyll and its transformation products in anoxic sediments. Geochim Cosmochim Acta 64:2281–2294
Vinebrooke RD, Hall RI, Leavitt PR, Cumming BF (1998) Fossil pigments as indicators of phototrophic response to salinity and climatic change in lakes of western Canada. Can J Fish Aquat Sci 55:668–681
Vinebrooke RD, Leavitt PR (1999) Phytobenthos and phytoplankton as potential indicators of climate change in mountain lakes and ponds: a HPLC-based pigment approach. J N Am Benth Soc 18:15–33
Acknowledgements
We thank participants in the EMERGE project that contributed to the data set of environmental variables. We also thank C. Borrego for kindly providing us with photosynthetic bacteria cultures. We are grateful to M. Ventura, J. P. Smol and two anonymous reviewers for constructive comments on the manuscript and A. M. Poulsen for editorial assistance. The study was supported by the European Union within the framework of the EMERGE (Contract No. EVK1-CT-1999–00032) and EUROLIMPACS (GOCE-CT-2003–505540).
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Buchaca, T., Catalan, J. On the contribution of phytoplankton and benthic biofilms to the sediment record of marker pigments in high mountain lakes. J Paleolimnol 40, 369–383 (2008). https://doi.org/10.1007/s10933-007-9167-1
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DOI: https://doi.org/10.1007/s10933-007-9167-1