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Characterization of Protein Profiling and mRNA Expression of LLC Exosomes

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Abstract

Circulating exosomes are promising biomarker source in various diseases. Exosomal constituents can stably exist in the circulating plasma and serum thus making them ideal biomarkers for a number of clinical applications. Exosomes can also mediate the occurrence of many types of diseases, including distal cancerous metastasis and tumour enlargement, through encapsulated proteins or RNAs, which regulate interactions among tissues. While performing these actions, exosomes show tissue specificity. However, the mechanism for such selection is not clear. For non-small cell lung cancer (NSCLC), molecular diagnostic markers and mechanisms of exosome-mediated tumour metastasis are not well understood. Therefore, in this study, we characterized LLC exosomal proteins and mRNAs by analysing their molecular profiles, laying a foundation for exploring diagnostic markers of lung cancer. Furthermore, the interactions between exosomal membrane proteins and their target proteins were analysed and revealed a possible tissue propensity of LLC cell-derived exosomes. These findings provide a theoretical basis for studying exosome-mediated tissue targeting and distal lung cancer metastasis.

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Acknowledgements

We thank for Pei He and Shibei Wang collecting exosomes of LLC cells and running assays for following bioinformatics investigations in this study. We thanks Yujing Zhang designing and supervising this work.

Funding

This work was supported by grants from the National Natural Science Foundation of China (No. 31771666, 31741066, 31200969) and the Industrialization of Mongolian Medicine of “Prairie Talents” engineering (NEI ZU TONG ZI No. 2015-56).

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Authors and Affiliations

  1. State Key Laboratory of Pharmaceutical Biotechnology, Collaborative Innovation Center of Chemistry for Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing University, 163 Xianlin Road, Nanjing, 210023, Jiangsu, China

    Wen Zhang, Pei He, Shibei Wang, Amila Adili, Zixuan Chen, Chen-Yu Zhang, Xiaohong Jiang, Jing Li & Yujing Zhang

  2. Innovative Mongolian Medical Engineering Research Centre, International Mongolian Hospital of Inner Mongolia, 83 East University Road, Sai Han District, Hohhot, 010020, Inner Mongolia, China

    Wen Zhang

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  1. Wen Zhang
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Zhang, W., He, P., Wang, S. et al. Characterization of Protein Profiling and mRNA Expression of LLC Exosomes. Protein J 38, 586–597 (2019). https://doi.org/10.1007/s10930-019-09849-0

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