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A Calcium Ion-Dependent Dimeric Bean Lectin with Antiproliferative Activity Toward Human Breast Cancer MCF-7 Cells

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Abstract

In this study, a 60.8-kDa dimeric lectin was isolated from the Phaseolus vulgaris cv. jade bean and characterized. The lectin was bound on Blue Sepharose 6 and Q Sepharose and was finally purified by size exclusion chromatography on Superdex 200. Its hemagglutinating activity toward rabbit erythrocytes was dependent on divalent cations, especially calcium ions. Various carbohydrates tested were devoid of any effect on the hemagglutinating activity. The lectin was stable at pH between 4.5 and 9.4 and temperatures between 30 and 70 °C. It did not exert antifungal activity toward Valsa mali, Setosphaeria turcica, Mycosphaerella arachidicola, Fusarium oxysporum and Bipolaris maydis. The IC50 of the antiproliferative activity of the lectin toward MCF-7 human breast cancer cells was 174 μM. It did not inhibit proliferation of WRL-68 human normal embryonic hepatocytes. The lectin was dependent on calcium ions for hemagglutinating activity and possessed a blocked N-terminus. These two characteristics make the lectin unique among Phaseolus lectins.

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Abbreviations

FPLC-SEC:

Fast protein liquid chromatography-size exclusion chromatography

HA:

Hemagglutinating activity

IC50 :

The half maximal inhibitory concentration

ML:

Mistletoe lectin

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Authors and Affiliations

  1. School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

    Randy Chi Fai Cheung, Wen Liang Pan & Tzi Bun Ng

  2. Department of Medicine, Imperial College London, London, UK

    Ho Him Leung

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  1. Randy Chi Fai Cheung
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  2. Ho Him Leung
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  3. Wen Liang Pan
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  4. Tzi Bun Ng
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Correspondence to Tzi Bun Ng.

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Cheung, R.C.F., Leung, H.H., Pan, W.L. et al. A Calcium Ion-Dependent Dimeric Bean Lectin with Antiproliferative Activity Toward Human Breast Cancer MCF-7 Cells. Protein J 32, 208–215 (2013). https://doi.org/10.1007/s10930-013-9477-2

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  • DOI: https://doi.org/10.1007/s10930-013-9477-2

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