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Antifungal and Other Biological Activities of Two 2S Albumin-Homologous Proteins Against Pathogenic Fungi

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Abstract

The aim of this study was to determine whether 2S albumins from Passiflora edulis f. flavicarpa and Capsicum annuum seeds inhibit growth, induce plasma membrane permeabilization and induce endogenous production of nitric oxide in different pathogenic and non-pathogenic yeasts. The 2S albumin from P. flavicarpa (Pf-Alb) inhibited the growth of Kluyveromyces marxiannus, Candida albicans and Candida parapsilosis. The membranes of these yeast strains were permeabilized in the presence of Pf-Alb. The Pf-Alb also inhibited the glucose-stimulated acidification of the medium by Saccharomyces cerevisiae and C. albicans cells, which indicates a probable impairment of fungal metabolism because the inhibition of acidification occurred at various Pf-Alb concentrations and pre-incubation times. The 2S albumin from C. annuum (Ca-Alb) inhibited the growth of the yeasts K. marxiannus, C. tropicalis, C. albicans and S. cerevisiae. These yeast strains exhibited NO induction in the presence of Ca-Alb and displayed cellular agglomeration, elongated cells and the induction of pseudohyphae. Pf-Alb and Ca-Alb at various concentrations also inhibited the glucose-stimulated acidification of the medium by S. cerevisiae cells. Our results indicate that the ability of antimicrobial plant proteins such as 2S albumins to induce microbial inhibition could be an important factor in determining pathogen virulence. Therefore, 2S albumins might be targets for the design of new antifungal drugs.

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Abbreviations

Ca-Alb:

2S albumin-homologous protein from chili pepper seeds

DAF-2 DA:

3,4-diaminomethyl-2′,7′-difluoroescein diacetate

DIC:

Differential interference contrast

HPLC:

High-performance liquid chromatography

NO:

Nitric Oxide

Pf-Alb:

2S albumin-homologous protein from passion fruit

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Acknowledgments

We acknowledge the financial support of the Brazilian agencies CNPq, CAPES and FAPERJ. We are grateful to L. C. D. Souza for technical assistance.

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Authors and Affiliations

  1. Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, 28013-602, Brazil

    Suzanna F. F. Ribeiro, Gabriel B. Taveira, André O. Carvalho, Germana B. Dias, Maura Da Cunha, Claudete Santa-Catarina & Valdirene M. Gomes

  2. Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, 28013-602, Brazil

    Rosana Rodrigues

Authors
  1. Suzanna F. F. Ribeiro
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  2. Gabriel B. Taveira
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  3. André O. Carvalho
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  4. Germana B. Dias
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  5. Maura Da Cunha
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  6. Claudete Santa-Catarina
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  7. Rosana Rodrigues
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  8. Valdirene M. Gomes
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Corresponding author

Correspondence to Valdirene M. Gomes.

Additional information

Suzanna F. F. Ribeiro and Gabriel B. Taveira contributed equally to this work.

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Ribeiro, S.F.F., Taveira, G.B., Carvalho, A.O. et al. Antifungal and Other Biological Activities of Two 2S Albumin-Homologous Proteins Against Pathogenic Fungi. Protein J 31, 59–67 (2012). https://doi.org/10.1007/s10930-011-9375-4

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  • DOI: https://doi.org/10.1007/s10930-011-9375-4

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