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Bactericidal Activity Identified in 2S Albumin from Sesame Seeds and In silico Studies of Structure–Function Relations

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Abstract

Pathogenic bacteria constitute an important cause of hospital-acquired infections. However, the misuse of available bactericidal agents has led to the appearance of antibiotic-resistant strains. Thus, efforts to seek new antimicrobials with different action mechanisms would have an enormous impact. Here, a novel antimicrobial protein (SiAMP2) belonging to the 2S albumin family was isolated from Sesamum indicum kernels and evaluated against several bacteria and fungi. Furthermore, in silico analysis was conducted in order to identify conserved residues through other 2S albumin antimicrobial proteins (2S-AMPs). SiAMP2 specifically inhibited Klebsiella sp. Specific regions in the molecule surface where cationic (RR/RRRK) and hydrophobic (MEYWPR) residues are exposed and conserved were proposed as being involved in antimicrobial activity. This study reinforces the hypothesis that plant storage proteins might also play as pathogen protection providing an insight into the mechanism of action for this novel 2S-AMP and evolutionary relations between antimicrobial activity and 2S albumins.

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Abbreviations

AMP:

Antimicrobial peptide/protein

RF:

Protein rich fraction

NRP:

Non-retained proteins

RP:

Retained proteins

MIC:

Minimum inhibitory concentration

IPG:

Immobilized pH gradient

SiAMP2:

Antimicrobial protein from Sesamum indicum

CFU:

Colony forming units

MP:

Maximum Parsimony method

2S-AMP:

Antimicrobial 2S albumin

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Acknowledgments

This work was supported by CAPES, CNPq, FAPDF, FAPEMIG and UCB.

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Authors and Affiliations

  1. Centro de Análises Proteômicas e Bioquímicas, Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN Quadra 916, Modulo B, Brasília, Distrito Federal, 70790-160, Brazil

    Simone Maria-Neto, Rodrigo V. Honorato, Fábio T. Costa, Renato G. Almeida, Daniel S. Amaro & Octávio L. Franco

  2. Pós-graduação em Patologia Molecular, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil

    Daniel S. Amaro & Octávio L. Franco

  3. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Av. da Universidade 2853, Benfica, Fortaleza, Ceará, 60020-181, Brazil

    José T. A. Oliveira & Ilka M. Vasconcelos

  4. Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer s/n, Campus Universitário Bairro São Pedro, Juiz de Fora, Minas Gerais, 36015-260, Brazil

    Octávio L. Franco

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  1. Simone Maria-Neto
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Maria-Neto, S., Honorato, R.V., Costa, F.T. et al. Bactericidal Activity Identified in 2S Albumin from Sesame Seeds and In silico Studies of Structure–Function Relations. Protein J 30, 340–350 (2011). https://doi.org/10.1007/s10930-011-9337-x

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