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Polymer Films of Poly-3-hydroxybutyrate Synthesized by Cupriavidus necator from Different Carbon Sources

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Abstract

Films were prepared from 2% solutions of biodegradable poly-3-hydroxybutyrate [P(3HB)] and investigated. The polymer was synthesized by the Cupriavidus necator B-10646 bacterium cultivated using various carbon sources (glucose and glycerol of different degrees of purity, containing 0.3 to 17.93% impurities). Glycerol as the substrate influenced molecular-weight properties and crystallinity of the polymer without affecting its temperature characteristics. The P(3HB) specimens synthesized from glycerol had reduced Mw (300–400 kDa) and degree of crystallinity (50–55%) compared to the specimens synthesized from glucose (860 kDa and 76%, respectively). The low-crystallinity P(3HB) specimens, regardless of the degree of purity of glycerol, produced a beneficial effect on the properties of polymer films, which had a better developed folded surface and increased hydrophilicity. The values of the highest roughness (Ra) of the films synthesized from glycerol were 1.8 to 4.0 times lower and the water angles 1.4–1.6 times smaller compared to the films synthesized from glucose (71.75 nm and 87.4°, respectively). Those films performed better as cell scaffolds: the number of viable NIH fibroblasts was 1.7–1.9 times higher than on polystyrene (control) or films of P(3HB) synthesized from glucose.

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Acknowledgements

The reported study was funded by RFBR and KKRF Grant No. 19-43-240012 “Biological and physical principles of production of new generation biomaterials”. The work was carried out as part of the State Assignment of the Ministry of Education and Science of the Russian Federation No. FSRZ-2020-0006. The authors would like to express their special thanks to Krasnoyarsk Regional Center of Research Equipment of Federal Research Center «Krasnoyarsk Science Center SB RAS» for providing equipment to ensure the accomplishment of this project.

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Authors and Affiliations

  1. Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, Russia, 660041

    Ekaterina Shishatskaya, Alexander Vasiliev, Evgeniy Kiselev, Aleksey Sukovatyi & Tatiana Volova

  2. Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russia

    Ekaterina Shishatskaya, Evgeniy Kiselev, Aleksey Sukovatyi & Tatiana Volova

  3. L.V. Kirenskii Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russia

    Ivan Nemtsev, Anna Lukyanenko & Alexander Vasiliev

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  1. Ekaterina Shishatskaya
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Correspondence to Tatiana Volova.

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Shishatskaya, E., Nemtsev, I., Lukyanenko, A. et al. Polymer Films of Poly-3-hydroxybutyrate Synthesized by Cupriavidus necator from Different Carbon Sources. J Polym Environ 29, 837–850 (2021). https://doi.org/10.1007/s10924-020-01924-3

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