Abstract
Halophilic microorganisms produce several metabolites having potential application in biotechnology and industry. They also produce polyhydroxyalkanoates (PHA), which are suitable replacements for conventional plastics for packaging food and non-food materials due to their biocompatible and biodegradable properties. PHA produced by the native isolate of Halomonas venusta KT832796 using a minimal medium was made into a film. It was characterized using Fourier Transform Infrared (FTIR) spectroscopy, Nuclear Magnetic Resonance (NMR) spectroscopy, GC–MS, UTM (Mechanical strength), Differential Scanning Calorimetry (DSC) and X-ray diffraction (XRD) analysis. Based on FTIR, NMR and GC–MS analyses the polymer produced by Halomonas venusta KT832796 was found to be poly(3-hydroxybutyrate). The polymer had a tensile strength of 26 MPa and Young’s modulus of 3 GPa and the melting temperature Tm 173.59 °C, which are found to be similar to the standard PHB polymer and the crystalline state was revealed by XRD pattern. This PHB polymer can be used in food packaging systems and medical applications for drug delivery. This is the first communication on characterization of PHA produced by Halomonas venusta.
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Acknowledgements
The authors would like to express their gratitude to the Director, CSIR-CFTRI for providing facilities to carry out the research work. Ms. Angelina Stanley would like to thank the funding agency of UGC-Maulana Azad National Fellowship, for financially supporting the research work.
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Stanley, A., Murthy, P.S.K. & Vijayendra, S.V.N. Characterization of Polyhydroxyalkanoate Produced by Halomonas venusta KT832796. J Polym Environ 28, 973–983 (2020). https://doi.org/10.1007/s10924-020-01662-6
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DOI: https://doi.org/10.1007/s10924-020-01662-6