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Characterization of Atrazine-Loaded Biodegradable Poly(Hydroxybutyrate-Co-Hydroxyvalerate) Microspheres

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Abstract

Polyhydroxybutyrate-co-hydroxyvalerate microspheres (PHBV-MS) were prepared as a delivery system for the herbicide atrazine (ATZ). Characterization of the system included investigation of in vitro release properties and genotoxicity. ATZ − PHBV-MS particle diameters showed a size distribution range of 1–13 μm. Differential scanning calorimetry analyses indicated that ATZ was associated with the PHBV microparticles. The release profiles showed a different release behavior for the pure herbicide in solution, as compared with that containing ATZ-loaded PHBV-MS. Korsmeyer–Peppas model analyses showed that atrazine release from the microparticles occurred by a combination of diffusion through the matrix and partial diffusion through water-filled pores of the PHBV microparticles. A Lactuca sativa test result showed that the genotoxicity of ATZ-loaded PHBV-MP was decreased in relation to ATZ alone. The results demonstrate a viable biodegradable herbicide release system using atrazine for agrochemical purposes.

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Acknowledgments

This research was supported by FAPESP, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) and Fundunesp. RG and NFSM (fellowships 08/01222-9 and 06/00121-0, respectively) are also grateful to FAPESP. AHR and LFF are recipients of fellowship from CNPq. The authors would like to thank Dr. Luiz H. C. Mattoso (Embrapa-CNPDIA) for facilitating use of the DSC equipment.

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Authors and Affiliations

  1. Unesp - Univ. Estadual Paulista, Departamento de Engenharia Ambiental, Campus Sorocaba, Av. Três de Março, 511, CEP 18087-180, Sorocaba, SP, Brazil

    Renato Grillo, Nathalie F. S. de Melo, Roberto Wagner Lourenço, André Henrique Rosa & Leonardo Fernandes Fraceto

  2. Departamento de Bioquímica, Instituto de Biologia, Unicamp, Cidade Universitária Zeferino, Vaz, s/n, Campinas, SP, Brazil

    Renato Grillo, Nathalie F. S. de Melo & Leonardo Fernandes Fraceto

  3. Departamento de Biotecnologia, Universidade de Sorocaba, Sorocaba, SP, Brazil

    Renata de Lima

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  1. Renato Grillo
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  2. Nathalie F. S. de Melo
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  3. Renata de Lima
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  4. Roberto Wagner Lourenço
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  5. André Henrique Rosa
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  6. Leonardo Fernandes Fraceto
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Correspondence to Leonardo Fernandes Fraceto.

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Grillo, R., de Melo, N.F.S., de Lima, R. et al. Characterization of Atrazine-Loaded Biodegradable Poly(Hydroxybutyrate-Co-Hydroxyvalerate) Microspheres. J Polym Environ 18, 26–32 (2010). https://doi.org/10.1007/s10924-009-0153-8

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  • DOI: https://doi.org/10.1007/s10924-009-0153-8

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