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Bioformulated Hesperidin-Loaded PLGA Nanoparticles Counteract the Mitochondrial-Mediated Intrinsic Apoptotic Pathway in Cancer Cells

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Abstract

In this study, a biological approach has carried out to develop the polymer-based nanoparticles using a bioflavonoid of hesperidin. This formulation has been attempted and established for its biomedical application in cancer treatment. Hesperidin loaded PLGA nanoparticles (Hes-PLGA-NPs) were processed by oil in water single emulsion solvent evaporation technique and the physiochemical traits were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), XRD and NMR analysis. DLS and TEM analysis revealed that the preparation yielded a spherical shaped nanoparticle with an average measure of 38.4 nm. The XRD spectrum showed a crystalline type of nanoparticles. The FTIR and NMR investigation exposed that the PLGA also serves up as capping or switching material for pH-dependent drug release. Furthermore, the cationic natures of Hes-PLGA-NPs easily penetrate cancerous cells and efficiently repressed the growth in acidic conditions. Taking into account, the findings of in vitro studies in HEp-2 cell suggested that bioformulated Hes-PLGA-NPs are more competent than native hesperidin and might be used as a potent anti-cancer candidate. Further studies are warranted to expose the toxicity and molecular level mechanisms involved in the anti-cancer activity of the synthesized Hes-PLGA-NPs as nanomedicine.

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  • 31 October 2020

    The authors noticed an inadvertent error in our article “Bioformulated Hesperidin-Loaded PLGA Nanoparticles Counteract the Mitochondrial-Mediated Intrinsic Apoptotic Pathway in Cancer Cells”.

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Authors and Affiliations

  1. Department of Biochemistry, Sacred Heart College (Autonomous), Tirupattur, Tamil Nadu, 635 601, India

    Krithiga Balakrishnan & Anbu Singaravelu

  2. Department of Biochemistry, Thiruvalluvar Arts and Science College, Kurinjipadi, Cuddalore, Tamil Nadu, 607302, India

    Krithiga Balakrishnan

  3. Department of Physics, Sri Padmavati Mahila Visvavidyalayam (Women’s University), Tirupati, Andhra Pradesh, 517502, India

    Sangeetha C. Casimeer

  4. Department of Pharmacy, Aqaba University of Technology, Aqaba, Jordan

    Alaa Yousef Ghidan

  5. Pharmaceutical Service Administration, Quality and Medication Safety Unit, King Abdullah Medical City, Makkah, Saudi Arabia

    Fatima Yousef Ghethan

  6. Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, UAE University, Al Ain, United Arab Emirates

    Karthikkumar Venkatachalam

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  1. Krithiga Balakrishnan
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  3. Alaa Yousef Ghidan
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Balakrishnan, K., Casimeer, S.C., Ghidan, A.Y. et al. Bioformulated Hesperidin-Loaded PLGA Nanoparticles Counteract the Mitochondrial-Mediated Intrinsic Apoptotic Pathway in Cancer Cells. J Inorg Organomet Polym 31, 331–343 (2021). https://doi.org/10.1007/s10904-020-01746-9

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