Abstract
Nano bioactive glasses in the system; 70 SiO2–10 P2O5–(20–x) CaO–xCuO (x = 0, 3 and 5% mol%), were synthesized by the rapid alkali sol–gel method. The dual effect of incorporating 0, 3, and 5 mol% of CuO (replacing CaO) and gamma irradiation on nanobioactive glasses structure were studied. The bioactivity, cytotoxicity and promotion in wound healing of fibroblast cells (BJ-1) were studied. The results showed that the unirradiated and irradiated samples were able to form hydroxyapatite layer on their surfaces in SBF after 30 days. Scratch-wound healing assay also, indicated that the samples which incorporating 3 mol% of CuO with or without gamma irradiation had the highest wound closure rate (99.1%, and 98.1%, respectively). The result of cytotoxic assay demonstrated that 100 μg/mL of 0, 3 mol% of CuO before and after gamma irradiation showed non-significant decrease (p > 0.05) in the mean viability percentage towards BJ.1 cells. Therefore, these compositions could be suitable for wound healing applications.
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Acknowledgements
The authors thank the National Research Centre, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority (EAEA) for the possibility to use their equipment and facilities. And also, deepest thanks Dr. Eman Fayez Said Taha, Health Radiation Research Department, National Centre for Radiation Research and Technology, Egyptian Atomic Energy Authority (EAEA), for her cooperation and encouragement through this study. Recommendation: Further studies should be done to the stage of pre-clinical and clinical trials. Possible medical treatments can be tested in vitro before beginning in vivo studies by studying the wound healing process.
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Ahmed, M.M., Abd-Allah, W.M., Omar, A.E. et al. The Dual Effect of Copper and Gamma Irradiation on Chronic Wound Healing of Nanobioactive Glass. J Inorg Organomet Polym 30, 3646–3657 (2020). https://doi.org/10.1007/s10904-020-01501-0
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DOI: https://doi.org/10.1007/s10904-020-01501-0