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Photochemically-Generated Silver Chloride Nanoparticles Stabilized by a Peptide Inhibitor of Cell Division and Its Antimicrobial Properties

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Abstract

The 40 amino acid residues peptide MciZ (Mother Cell Inhibitor of FtsZ) represents a promising antibacterial agent as it is an effective inhibitor of bacterial cell division, Z-ring formation and localization. However, its efficacy is limited to Gram-positive bacteria as MciZ is unable to penetrate into Gram-negative organisms. In this study, we report the synthesis of plasmonic silver chloride nanoparticles stabilized by MciZ (NP/MciZ). NP/MciZ were synthesized using a fast and green route under blue light irradiation. Electron microscopy showed that NP/MciZ were enveloped by a peptide layer responsible for colloidal stability. X-ray diffraction analysis showed that silver chloride nanoparticles were crystalline in nature with small proportion of metallic silver. NP/MciZ showed a dose-dependent cytotoxicity against mammalian VERO cells. However, NP/MciZ exhibited remarkable antibacterial activity against Gram-positive bacterium B. megaterium comparable to MciZ. Furthermore, NP/MciZ showed similar antimicrobial activity against the Gram-negative bacterium E. coli and the yeast C. albicans. The photochemically-generated NP/MciZ presented here is a new organic–inorganic hybrid nanomaterial and has potential for biomedical or other applications.

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Acknowledgements

The authors are grateful to the Multiuser Central Facilities (UFABC). We thank Bruno L. Batista for help with ICP-MS measurements and Aryane Tofanello for help with the ImageJ software. Cesar de la Fuente-Nunez holds a Presidential Professorship at the University of Pennsylvania and is a recipient of the Langer Prize by the AIChE Foundation. We acknowledge funding from the Institute for Diabetes, Obesity, and Metabolism of the University of Pennsylvania.

Funding

We would like to thank Fundação de Amparo à Pesquisa do Estado de São Paulo (via Grants 2013/12338-6 and 2017/17275-3) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (via Grant 305740/2017-2) for financial support.

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Author notes

  1. Geomar F. Cruz and Iris de Araujo have contributed equally to this work.

Authors and Affiliations

  1. Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, SP, Brazil

    Geomar F. Cruz, Vani X. Oliveira Jr., Dnane V. Almeida & Wanius Garcia

  2. Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, São Bernardo do Campo, SP, Brazil

    Iris de Araujo, Felipe N. Ambrosio & Fernanda D. Silva

  3. Machine Biology Group, Departments of Psychiatry and Microbiology, Institute for Biomedical Informatics, Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, Penn Institute for Computational Science, and Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, 19104, USA

    Marcelo D. T. Torres & Cesar de la Fuente-Nunez

  4. Centro de Engenharia, Modelagem e Ciências Sociais Aplicadas, Universidade Federal do ABC, São Bernardo do Campo, SP, Brazil

    Christiane B. Lombello

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  1. Geomar F. Cruz
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Cruz, G.F., de Araujo, I., Torres, M.D.T. et al. Photochemically-Generated Silver Chloride Nanoparticles Stabilized by a Peptide Inhibitor of Cell Division and Its Antimicrobial Properties. J Inorg Organomet Polym 30, 2464–2474 (2020). https://doi.org/10.1007/s10904-019-01427-2

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