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A Novel L-Arginine Functionalized CdTe Quantum Dots Fluorescence Probe for Pyrophosphate Anion Detection

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Abstract

In this paper, a novel amino acid surface-functionalized semiconductor CdTe quantum dot fluorescent probe amidated by carboxyl and amide groups was synthesized to detect pyrophosphate ions (P2O74−, PPi). L-Arginine (L-Arg) was grafted onto cysteine modified CdTe quantum dots (Mea-CdTe QDs) to form a new L-Arginine-functionalized quantum dot fluorescent probe (L-Arg@Mea-CdTe). The prepared probe has good optical properties with multiple grafted functional groups on the surface. The guanidine group of the L-Arg@Mea-CdTe fluorescent probe is strongly basic and will be fully protonated under physiological conditions. The resulting hydrogen bonds bound to pyrophosphate lead to significant changes in the fluorescence of CdTe quantum dots. IR and XPS characterization were performed to confirm it. The addition of PPi induces a significant fluorescence quenching of L-Arg@Mea-CdTe in aqueous solution. The fluorescent QDs probe can also detect pyrophosphate with good sensitivity and anti-interference performance. The detection limit of the L-Arg@Mea-CdTe fluorescence probe for PPi is as low as 0.30 μM. In addition, the novel nano-fluorescent probe was successfully applied to detect PPi in water and in cell imaging.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This research is financially supported by the Anhui Province University Natural Science Research Project (No. KJ2020A0014) and the Anhui Provincial Natural Science Foundation (No. 1808085ME155).

Funding

This work was supported by the Anhui Province University Natural Science Research Project (No. KJ2020A0014) and the Anhui Provincial Natural Science Foundation (No. 1808085ME155).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Anhui University, Hefei, 230601, People’s Republic of China

    Ting Chen, Zicheng Ge, Min Fang & Weiju Zhu

  2. School of Materials Science and Engineering, Anhui University, Hefei, 230601, People’s Republic of China

    Cun Li

  3. AnHui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Anhui University, Hefei, 230601, People’s Republic of China

    Weiju Zhu & Cun Li

  4. Anhui Province Key Laboratory of Environment-Friendly Polymer Materials, Anhui University, Hefei, 230601, People’s Republic of China

    Min Fang

Authors
  1. Ting Chen
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  2. Zicheng Ge
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  3. Min Fang
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Ting Chen, Zicheng Ge. The first draft of the manuscript was written by Ting Chen and revised by Weiju Zhu and Min Fang; Cun Li and Min Fang gave writing- reviewing and experimental platform, supervision. All authors read and approved the final manuscript.

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Correspondence to Min Fang or Cun Li.

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Zicheng Ge Co-first author.

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Chen, T., Ge, Z., Fang, M. et al. A Novel L-Arginine Functionalized CdTe Quantum Dots Fluorescence Probe for Pyrophosphate Anion Detection. J Fluoresc 33, 2075–2084 (2023). https://doi.org/10.1007/s10895-023-03198-z

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