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An HBT-Based Near-Infrared Fluorescent Probe for Colorimetric and Ratiometric Detection of Bisulfite and its Application in Living Cells

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Abstract

In this paper, a new type of ratiometric fluorescent probe HBT-TCF with large emission shift (up to 200 nm) has been developed for sensing HSO3 − based on Michael-type addition reaction. The probe could fast recognize of HSO3 − within 3 min in PBS buffer solution. The detection limit of the probe for HSO3 − is as low as 101 nM. Meanwhile, the sensing mechanism is confirmed by NMR, LCMS. In addition, the probe can be applied to detect HSO3 − in living cells.

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Acknowledgment

This work was supported by the Key Project of the Education Department of Hubei Province (No. Q2015010).

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Authors and Affiliations

  1. Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, People’s Republic of China

    Yuanchao Sun, Zhen Chen, Fengzao Chen, Heng Liu, Hanping He, Xiuhua Zhang & Shengfu Wang

Authors
  1. Yuanchao Sun
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  2. Zhen Chen
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  3. Fengzao Chen
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  4. Heng Liu
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  5. Hanping He
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  6. Xiuhua Zhang
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  7. Shengfu Wang
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Corresponding author

Correspondence to Heng Liu.

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Sun, Y., Chen, Z., Chen, F. et al. An HBT-Based Near-Infrared Fluorescent Probe for Colorimetric and Ratiometric Detection of Bisulfite and its Application in Living Cells. J Fluoresc 27, 1405–1411 (2017). https://doi.org/10.1007/s10895-017-2077-3

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  • DOI: https://doi.org/10.1007/s10895-017-2077-3

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