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A Capillary Based Chemiluminscent Multi-Target Immunoassay

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Abstract

Renewed interest in capillary format immunoassays has lead to increasingly costly and complex approaches to preparation and readout. This study describes a simple multi-target method based on a capillary platform using horseradish peroxidase (HRP) labelled IgG to visualize an antibody antigen complex. When goat-anti-human IgG was employed as the probe and human IgG as target, the system allowed detection of target to less than 1 ng/mL using a standard detection approach. The capillaries were read visually or with a commercial grade CCD camera. Multi-target detection was demonstrated using a model system of rat-anti-mouse, goat-anti-human and mouse-anti-rat IgG. These probes were encoded to different locations in the capillary, providing a simple inexpensive approach to achieve multi-target assays.

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Abbreviations

PCR:

Polymerase chain reaction

ELISA:

Enzyme-linked immunosorbent assay

HRP:

Horseradish peroxidase

TMB:

3,3,5,5,-tetramethylbenzidine

CCD:

Charge-coupled device

PBS:

Phosphate Buffered Saline

BSA:

Bovine Serum Albumin

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Authors and Affiliations

  1. Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Jianghan University, Wuhan, 430056, China

    Yuan-Cheng Cao

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  1. Yuan-Cheng Cao
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Correspondence to Yuan-Cheng Cao.

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Cao, YC. A Capillary Based Chemiluminscent Multi-Target Immunoassay. J Fluoresc 25, 563–568 (2015). https://doi.org/10.1007/s10895-015-1535-z

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  • DOI: https://doi.org/10.1007/s10895-015-1535-z

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