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Long Wavelength Fluorescence Ratiometric Zinc Biosensor

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Abstract

A protein-based emission ratiometric fluorescence biosensor is described that exhibits sensitivity to free zinc ion in solution down to picomolar concentrations. Ratiometric measurements are widely used to assure accurate quantitation, and emission ratios are preferred for laser scanning microscopes such as confocal fluorescence microscopes. The relatively long emission wavelengths used are well suited to studies in tissues and other matrices which exhibit significant fluorescence background, and the apo-carbonic anhydrase moiety recognizes zinc ion with high and controllable specificity.

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Acknowledgments

The authors wish to thank the National Institutes of Health (NIH RO1 EB003924) for their support.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 N. Greene Street, Baltimore, MD, 21201, USA

    Hui Hui Zeng, Evgenia G. Matveeva & Richard B. Thompson

  2. Department of Chemistry, University of Michigan, 930 W. University Ave., Ann Arbor, MI, 47805, USA

    Andrea K. Stoddard & Carol A. Fierke

Authors
  1. Hui Hui Zeng
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  2. Evgenia G. Matveeva
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  3. Andrea K. Stoddard
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  4. Carol A. Fierke
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  5. Richard B. Thompson
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Correspondence to Richard B. Thompson.

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Zeng, H.H., Matveeva, E.G., Stoddard, A.K. et al. Long Wavelength Fluorescence Ratiometric Zinc Biosensor. J Fluoresc 23, 375–379 (2013). https://doi.org/10.1007/s10895-013-1161-6

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  • DOI: https://doi.org/10.1007/s10895-013-1161-6

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