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Exploring the Nature of Photo-Damage in Two-photon Excitation by Fluorescence Intensity Modulation

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Abstract

We investigate the relative photo-damage effects during one- and two-photon excitations and demonstrate that there exist fundamental differences in the damage induced by a high repetition rate laser as compared to that of a CW laser. This difference is evident from the degree of enhanced fluorescence intensity achieved by blanking the excitation with an optical chopper. Such an enhancement in fluorescence intensity provides better signal-to-noise ratio that could have immediate applications in multiphoton imaging of live specimens.

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Acknowledgements

AKD thanks CSIR, India for graduate fellowship. The authors thank MCIT and DST, India and Wellcome Trust Foundation, UK for funding. We acknowledge Debjit Roy for his assistance.

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  1. Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, UP-208016, India

    Arijit Kumar De & Debabrata Goswami

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  1. Arijit Kumar De
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  2. Debabrata Goswami
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Correspondence to Debabrata Goswami.

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De, A.K., Goswami, D. Exploring the Nature of Photo-Damage in Two-photon Excitation by Fluorescence Intensity Modulation. J Fluoresc 19, 381–386 (2009). https://doi.org/10.1007/s10895-008-0405-3

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  • DOI: https://doi.org/10.1007/s10895-008-0405-3

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