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Cellular Uptake of Fluorescent Labelled Biotin–Streptavidin Microspheres

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Abstract

Amino functionalized, cross-linked, polystyrene microspheres were covalently loaded with streptavidin to which was coupled fluorescently labeled biotin and biotinylated-tagged DNA. These biotin–streptavidin microsphere conjugates were then successfully delivered into cells. The application of the streptavidin–biotin technology to these microspheres allows the effective delivery of any biotinylated material into intact mammalian cells, without the need for delicate procedures such as micro-injection.

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Acknowledgments

This research was supported by the EPSRC. R.M.S.-M. thanks the Royal Society for a Dorothy Hodgkin Research Fellowship.

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Authors and Affiliations

  1. Chemical Biology Section, School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, EH9 3JJ, Edinburgh, UK

    M. Bradley, L. Alexander & R. M. Sanchez-Martin

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  1. M. Bradley
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  2. L. Alexander
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  3. R. M. Sanchez-Martin
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Correspondence to R. M. Sanchez-Martin.

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Bradley, M., Alexander, L. & Sanchez-Martin, R.M. Cellular Uptake of Fluorescent Labelled Biotin–Streptavidin Microspheres. J Fluoresc 18, 733–739 (2008). https://doi.org/10.1007/s10895-008-0334-1

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  • DOI: https://doi.org/10.1007/s10895-008-0334-1

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