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Interaction of a New Fluorescent Probe with DNA and its Use in Determination of DNA

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Abstract

In this paper we reported a metal complex 1-Zn (2,5-di-[2-(3,5-bis(2-pyridylmethyl)amine-4-hydroxy-phenyl)-ethylene]-pyrazine-Zn) as a fluorescent probe sensing DNA. The result of the competitive experiment of the probe with ethidium bromide (EB) to bind DNA, absorption spectral change and polarization change in the presence and absence of DNA revealed that interaction between the probe and DNA was via intercalation. Ionic strength experiment showed the existence of electrostatic interaction as well. Scatchard plots also confirmed the combined binding modes. The fluorescence enhancement of the probe was ascribed to highly hydrophobic environment when it bound the macromolecules such as DNA, RNA or denatured DNA. The binding constant between the probe and DNA was estimated as 3.13 × 107 mol−1 L. The emission intensity increase was proportional to the concentration of DNA. Based on this, the probe was used to determine the concentration of calf thymus DNA (ct-DNA). The corresponding linear response ranged from 2.50 × 10−7 to 4.75 × 10−6 mol L−1, and detection limit was 1.93 × 10−8 mol L−1 for ct-DNA.

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Acknowledgments

Financial support of this study by the State Key Laboratory of Chemo/Biosensing and Chemometrics of Hunan University (No. 2005018), Jiangxi Province Education Ministry Foundation(No. 2005-38) and JXNSF (0420041) are highly acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, Nanchang University, Nanchang, 330031, China

    Fang-Ying Wu & Fei-Yan Xie

  2. Center of Analysis and Testing, Nanchang University, Nanchang, 330031, China

    Yu-Mei Wu

  3. Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul, 151-747, Republic of Korea

    Jong-In Hong

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  1. Fang-Ying Wu
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  2. Fei-Yan Xie
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  3. Yu-Mei Wu
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  4. Jong-In Hong
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Correspondence to Fang-Ying Wu or Jong-In Hong.

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Wu, FY., Xie, FY., Wu, YM. et al. Interaction of a New Fluorescent Probe with DNA and its Use in Determination of DNA. J Fluoresc 18, 175–181 (2008). https://doi.org/10.1007/s10895-007-0261-6

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  • DOI: https://doi.org/10.1007/s10895-007-0261-6

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