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Bacteria Spectra Obtained by Laser Induced Fluorescence

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Abstract

Laser-induced fluorescence technique (LIF) is described for getting bacteria spectra in the liquid phase. An excimer laser pumping a dye-laser and a doubly frequency laser have been used, exciting the bacteria to 290 nm, and using a monochromator, 600 lines/mm grating, with a CCD to measure and obtain the fluorescence spectra. In this study, a laser induced fluorescence system to measure certain bioaerosols (bacteria) was optimised. Finally, a small bacteria fluorescence spectra collection is presented.

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References

  1. Hinds WC (1999) Aerosol technology: Properties, behavior, and measurement of airborne particles, 2nd edn. John Willey & Sons

  2. Parat S, Perdrix A, Baconnier P (1999) Relationships between air-conditioning, airbone microorganisms and health. Bull Acad Nat Med 183(2):327–344

    Google Scholar 

  3. Spurny KR (1994) On the chemical detection of bioaerosols. J Aerosol Sci 25(8):1533–1547

    Article  CAS  Google Scholar 

  4. Ge Z, Wexler AS, Johnston MV (1998) Laser desorption/ionization of single ultrafine multicomponent aerosols. Environ Sci Technol 32:3218–3223

    Article  CAS  Google Scholar 

  5. Jayne JT, Leard DC, Zhang X, Davidovits P, Smith KA, Kolb CE, Worsnop DR (2000) Development of an aerosol mass spectrometer for size and composition analysis of submicron particles. Aerosol Sci Technol 33(1–2):49–70

    Article  CAS  Google Scholar 

  6. Cheng YS, Barr EB, Fan BJ, Hargis PJ Jr, Arder DJ, O’Hern TJ, Torczynski JR, Tisone GC, Preppernau BL, Young SA, Radloff RJ (1999) Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy. Aerosol Sci Tech 30:186–201

    Google Scholar 

  7. Pan YI, Holler S, Chang RK, Hill SC, Pinnick RG, Niles S, Bottiger JR (1999) Single-shot fluorescence spectra of individual micrometer-sized bioaerosols iluminated by a 351- or a 266-nm ultraviolet laser. Opt Lett 24(2):116–118

    Google Scholar 

  8. Seaver M, Eversole JD, Hardgrove JJ, Cary WK Jr, Roselle DC (1999) Size and fluorescence measurements for field detection of biological aerosols. Aerosol Sci Tech 30(2):174–185

    Article  CAS  Google Scholar 

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Acknowledgments

Government of Spain, Ministerio de Educación y Ciencia (project REN2002-01439/CLI), University of La Rioja (projects API-02/07, API-03/07, API-04/05), Government of La Rioja (project ANGI 2001/36). Thanks to Dra. Carmen Torres research group from the University of La Rioja and Dr. Rafael Pereira from Innova Instrumentación S.L. This work has not been posible without the advices and the collaboration of Professors Jim Winefordner, Ben Smith and Nico Omenetto from the University of Florida (Gainesville, FL, USA).

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Authors and Affiliations

  1. University of La Rioja, Chemistry Department (Analytical Chemistry Area), C/Madre de Dios 51, 26006, Logroño, La Rioja, Spain

    Susana Cabredo & Alejandro Parra

  2. University of Zaragoza, Laser Analytical Spectroscopy Lab, Analytical Chemistry Department, C/Pedro Cerbuna 12, Building D, 50009, Zaragoza, Spain

    Jesús Anzano

Authors
  1. Susana Cabredo
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  2. Alejandro Parra
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  3. Jesús Anzano
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Correspondence to Susana Cabredo.

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Cabredo, S., Parra, A. & Anzano, J. Bacteria Spectra Obtained by Laser Induced Fluorescence. J Fluoresc 17, 171–180 (2007). https://doi.org/10.1007/s10895-007-0155-7

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  • DOI: https://doi.org/10.1007/s10895-007-0155-7

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