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Laser-Induced Fluorescence Imaging and Spectroscopy of GFP Transgenic Plants

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Abstract

Green fluorescent protein (GFP) and other fluorescent protein bioreporters can be used to monitor transgenes in plants. GFP is a valuable marker for transgene presence and expression, but remote sensing instrumentation for stand-off detection has lagged behind fluorescent protein marker biotechnology. However, both biology and photonics are needed for the monitoring technology to be fully realized. In this paper, we describe laser-induced fluorescence imaging and laser-induced fluorescence spectroscopy of GFP-transgenic plants in ambient light towards the application of remote sensing of transgenic plants producing GFP.

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Authors and Affiliations

  1. Department of Plant Sciences, University of Tennessee, Knoxville, Tennesssee

    C. Neal Stewart Jr., Reginald J. Millwood, Matthew D. Halfhill, Mentewab Ayalew & Vinitha Cardoza

  2. Department of Botany, University of Vermont, Burlington, Vermont

    Vinitha Cardoza

  3. Department Radiation Oncology, Wake Forest University School of Medicine, Winston Salem, North Carolina

    Mitra Kooshki

  4. DOE Special Technologies Lab, Bechtel Nevada, Santa Barbara, California

    Gene A. Capelle, Kevin R. Kyle, David Piaseki, Gregory McCrum & John Di Benedetto

  5. Department of Plant Sciences, University of Tennessee, 2431 Joe Johnson Dr, Knoxville, Tennesssee, 37996

    C. Neal Stewart Jr.

Authors
  1. C. Neal Stewart Jr.
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  2. Reginald J. Millwood
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  3. Matthew D. Halfhill
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  4. Mentewab Ayalew
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  6. Mitra Kooshki
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  7. Gene A. Capelle
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  10. Gregory McCrum
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  11. John Di Benedetto
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Correspondence to C. Neal Stewart Jr..

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Stewart, C.N., Millwood, R.J., Halfhill, M.D. et al. Laser-Induced Fluorescence Imaging and Spectroscopy of GFP Transgenic Plants. J Fluoresc 15, 697–705 (2005). https://doi.org/10.1007/s10895-005-2977-5

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  • DOI: https://doi.org/10.1007/s10895-005-2977-5

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