Abstract
A double immunohistochemical technique for the simultaneous detection of T- and B cells in paraffin-embedded mice tissues have been developed. This procedure is based on using fluorescent nano-crystals (q-dots). The benefit of using q-dots evolves from their unique fluorescence characteristics advantages: such as broad excitation spectrum, narrow emission band and high photo-bleaching threshold compare to organic fluorophores. T cells antigens (CD3) were stained using antibody-coated q-dots with max emission at 655 nm (GαRb-QD655). B cells antigens (CD45R/B220) were stained using streptavidin-coated q-dots with max emission at 585 nm (SA-QD585). The simultaneous detection of T- and B cells was demonstrated in paraffin-embedded lymph node using standard fluorescence microscope.
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Zahavy, E., Freeman, E., Lustig, S. et al. Double Labeling and Simultaneous Detection of B- and T Cells Using Fluorescent Nano-Crystal (q-dots) in Paraffin-Embedded Tissues. J Fluoresc 15, 661–665 (2005). https://doi.org/10.1007/s10895-005-2972-x
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DOI: https://doi.org/10.1007/s10895-005-2972-x