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Performance Validation for Microplate Fluorimeters

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Abstract

As the sophistication of instruments that make fluorimetric measurements on samples in microplates has increased, so has the need for methods to validate instrumental performance. This paper describes a solid-state validation microplate that tests multiple aspects of fluorescence performance, including signal linearity, gain, noise, sensitivity, wavelength accuracy, and polarization stability. Both the operating principles and the validation of the validation microplate are discussed.

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References

  1. A. K. Gaigalas et al. (2001). J. Res. Natl. Inst. Stand. Technol. 106, 381–389.

    Google Scholar 

  2. L. Wang et al. (2002). J. Res. Natl. Inst. Stand. Technol. 107, 339– 353.

    Google Scholar 

  3. A. Schwartz et al. (2002). J. Res. Natl. Inst. Stand. Technol. 107, 83–91.

    Google Scholar 

  4. J. Lakowicz (1999). Principles of Fluorescence Spectroscopy, 2nd Ed., Kluwer Academic/Plenum, New York.

    Google Scholar 

  5. J. Owicki (2000). J. Biomol. Screening 5, 297–306.

    Google Scholar 

  6. J. Miller (1981). In J. Miller (Ed.), Standards in Fluorescence Spectroscopy, Chapman & Hall, London, pp. 8–14.

    Google Scholar 

  7. W. Venable, Jr. and K. Eckerle (1979). Standard Reference Materials: Didymium Glass Filters for Calibrating the Wavelength Scale of Spectrophotometers—SRM 2009, 2010, 2013, and 2014, National Bureau of Standards, Washington, DC.

  8. W. J. Korzun and W. G. Miller (1986). Clin. Chem. 32, 162–165.

    Google Scholar 

  9. ASTM International (1972). Standard Test Method for Spectral Bandwidth and Wavelength Accuracy of Fluorescence Spectrometers (Reapproved 1998), ASTM International, West Conshohocken, PA.

  10. J. C. Travis et al. (2002). Anal. Chem. 74, 3408–3415.

    Google Scholar 

  11. A. Paladini and L. Erijman (1988). J. Biochem. Biophys. Methods 17, 61–66.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, California, 94089

    Robert Giebeler, Evelyn McGown & Todd French

  2. Owicki Consulting, 956 N. California Avenue, Palo Alto, California, 94303

    John C. Owicki

Authors
  1. Robert Giebeler
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  2. Evelyn McGown
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  3. Todd French
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  4. John C. Owicki
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Corresponding author

Correspondence to Robert Giebeler.

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Giebeler, R., McGown, E., French, T. et al. Performance Validation for Microplate Fluorimeters. J Fluoresc 15, 363–375 (2005). https://doi.org/10.1007/s10895-005-2631-2

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  • DOI: https://doi.org/10.1007/s10895-005-2631-2

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