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Decrypting Cryptic Click Beetle Species by Analysis of Sex Pheromones

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Abstract

Despite sex pheromones being highly species specific, their use as phylogenetic characters and a tool for the verification of species status are still relatively few compared to use of morphological and molecular characters. Earlier studies revealed that within the click beetle species Idolus picipennis, two types can be separated based on pheromone composition. Gas chromatography/mass spectrometry analysis of pheromone from a third type of Idolus revealed the presence of geranyl hexanoate and geranyl octanoate in a ratio of ca. 1:9. Neryl esters and farnesyl esters, present in the glands of the other two species, are absent in this type. In field experiments, males of all three types were attracted specifically to synthetic mixtures of pheromone resembling their own females. This suggests that cross attraction among different types is unlikely and indicates that they are likely distinct species. Using the large numbers of male beetles caught in pheromone traps, morphological differences between the species were studied and an identification key derived. This study highlights the role of sex pheromones as a powerful tool in integrative taxonomy and systematics to study the phylogenetic position and evolution of taxa and to determine the taxonomic status of cryptic species.

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References

  • Bach M (1852) Käferfauna für Nord- und Mitteldeutschland, mit besonderer Rücksicht auf die preussischen Rheinlande. Band 2. J. Hölscher, Koblenz

  • Borg-Karlson A-K, Agren L, Dobson H, Bergström G (1988) Identification and electroantennographic activity of sex-specific geranyl esters in an abdominal gland of female Agriotes obscurus (L.) and A. lineatus (L.) (Coleoptera, Elateridae). Experientia 44:531–534

    Article  CAS  Google Scholar 

  • Calam DH (1969) Species and sex-specific compounds from the heads of male bumblebees (Bombus spp.). Nature 221:856–857

    Article  CAS  PubMed  Google Scholar 

  • Cate PC (2007) Elateridae. In: Löbl I, Smetana A (eds) Catalogue of palaearctic Coleoptera, vol 4. Apollo Books, Stenstrup, pp 89–209

    Google Scholar 

  • Costa C, Lawrence JF, Rosa SP (2010) Elateridae leach, 1815. In: Leschen RAB, Beutel RG, Lawrence JF (eds) Handbook of zoology, Vol IV, Arthropoda: Insecta, Teilband 39, Coleoptera, beetles, vol 2, Morphology and systematics. Walter de Gruyter, Berlin, pp 75–103

    Google Scholar 

  • Dayrat B (2005) Towards integrative taxonomy. Biol J Linn Soc 85:407–415

    Article  Google Scholar 

  • Horak M, Whittle CP, Bellas TE, Rumbo ER (1988) Pheromone gland components of some australian tortricids in relation to their taxonomy. J Chem Ecol 14:1163–1175

    Article  CAS  PubMed  Google Scholar 

  • Howse PE, Stevens IDR, Jones OT (eds.) (1998) Practical applications of pheromones and other semiochemicals. In: Insect pheromones and their use in pest management. Chapman & Hall, London, pp 261–355

  • Ivastschenko II, Adamenko EA (1980) Place of pheromone formation in females of Selatosomus latus (Coleoptera, Elateridae). Russian J Zoo 59:225–228

    Google Scholar 

  • Jurenka R (2004) Insect pheromone biosynthesis. In: Schulz S (Ed.) The chemistry of pheromones and other semiochemicals I. Topics in current chemistry. Springer, Berlin, 239:97–132

  • Laibner S (2000) In: Elateridae of the Czech and Slovak Republics. Kabourek Nakladatelství, Zlin, p182

  • Lecocq T, Lhomme P, Michez D, Dellicour S, Valterova I, Rasmont P (2011) Molecular and chemical characters to evaluate species status of two cuckoo bumblebees: Bombus barbutellus and Bombus maxillosus (Hymenoptera, Apidae, Bombini). Syst Entomol 36:453–469

    Article  Google Scholar 

  • Lohse GA (1979) 34. Familie: Elateridae. In: Freude H, Harde K, LohseGA (eds.) Die Käfer Mitteleuropas, Band 6: Diversicornia. Goecke & Evers, Krefeld, pp 103–186

  • Mayr E (1942) In: Systematics and the origin of species. Columbia University Press, New York

  • Merivee E, Erm A (1993) Studies on sex pheromone gland morphology and pheromone components in female elaterid beetles Agriotes obscurus L. and Agriotes lineatus L. (Coleoptera, Elateridae). Proc Estonian Acad Sci Biol 42:108–117

    CAS  Google Scholar 

  • Mori K (2004) Pheromone synthesis. In: Schulz S (Ed.) The chemistry of pheromones and other semiochemicals I. Topics in current chemistry. Springer, Berlin 239:1–50

  • Padial JM, Miralles A, De la Riva I, Vences M (2010) The integrative future of taxonomy. Front Zoo 7:16

    Article  Google Scholar 

  • Platia (1994) In: Fauna d’Italia Coleoptera Elateridae. Edizioni calderini Bologna, pp 303–305

  • Renou M, Lalanne-Cassou B, Michelot D, Gordon G, Doré J-C (1988) Multivariate analysis of the correlation between noctuidae subfamilies and the chemical structure of their sex pheromones or male attractants. J Chem Ecol 14:1187–1215

    Article  CAS  PubMed  Google Scholar 

  • Sanders CJ (1971) Sex pheromone specificity and taxonomy of budworm moths (Choristoneura). Science 171:911–913

    Article  CAS  PubMed  Google Scholar 

  • Schlick-Steiner BC, Steiner FM, Seifert B, Stauffer C, Christian E, Crozier RH (2010) Integrative taxonomy: a multisource approach to exploring biodiversity. Annu Rev Entomol 55:421–438

    Article  CAS  PubMed  Google Scholar 

  • Schneider CA, Rasband WS, Eliceiri KW (2012) NIH image to ImageJ: 25 years of image analysis. Nat Methods 9:671–675

    Article  CAS  PubMed  Google Scholar 

  • Svensson GP, Wang H-L, Lassance J-M, Anderbrant O, Chen G-F, Gregorsson B, Guertin C, Harala E, Jirle EV, Liblikas I, Petko V, Roques A, Rosenberg O, Strong W, Voolma K, Ylioja T, Wang Y-J, Zhou X-M, Löfstedt C (2013) Assessment of genetic and pheromonal diversity of the Cydia strobilella species complex (Lepidoptera: Tortricidae). Syst Entomol 38:305–315

    Article  Google Scholar 

  • Tolasch T (2008) Sex pheromone of Ectinus aterrimus (Linné, 1761) (Coleoptera: Elateridae). Chemoecology 18:177–180

    Article  CAS  Google Scholar 

  • Tolasch T, von Fragstein M, Steidle JLM (2007) Sex pheromone of Elater ferrugineus L. (Coleoptera: Elateridae). J Chem Ecol 33:2156–2166

    Article  CAS  PubMed  Google Scholar 

  • Tolasch T, von Fragstein M, Steidle JLM (2010) Sex pheromone of Agriotes acuminatus (Stephens, 1830) (Coleoptera: Elateridae). J Chem Ecol 36:314–318

    Article  CAS  PubMed  Google Scholar 

  • Tolasch T, König C, von Fragstein M, Steidle JLM (2013) Identification of the sex pheromone of Idolus picipennis (Bach, 1852) revealed the presence of a cryptic sibling species. J Chem Ecol 39:1433–1440

    Article  CAS  PubMed  Google Scholar 

  • Tóth M (2013) Pheromones and attractants of click beetles: an overview. J Pest Sci 86:3–17

    Article  Google Scholar 

  • Tóth M, Furian L, Yatsynin VG, Ujvary I, Szarukan I, Imrei Z, Tolasch T, Franke W, Jossi W (2003) Identification of pheromones and optimization of bait composition for click beetle pests (Coleoptera: Elateridae) in Central and Western Europe. Pest Manag Sci 59:417–425

    Article  PubMed  Google Scholar 

  • Vuts J, Tolasch T, Furlan L, Bálintné Csonka É, Felföldi T, Márialigeti K, Toshova TB, Subchev M, Xavier A, Tóth M (2012) Agriotes proximus and A. lineatus (Coleoptera: Elateridae): a comparative study on the pheromone composition and cytochrome c oxidase subunit I gene sequence. Chemoecology 22:23–28

    Article  CAS  Google Scholar 

  • Witzgall P, Kirsch P, Cork A (2010) Sex pheromones and their impact on pest management. J Chem Ecol 36:80–100

    Article  CAS  PubMed  Google Scholar 

  • Wyatt TD (2014) Pheromones and animal behaviour: chemical signals and signatures, 2nd edn. Cambridge University Press, Cambridge

    Google Scholar 

Download references

Acknowledgments

Joachim Lau (Gönningen) discovered the Idolus populations in Saarhölzbach. We thank Kerstin König, Pawel Malec, and Claas-Thido Pfaff for support in fieldwork. Christian König was financially supported by the Landesgraduiertenförderung Baden-Württemberg.

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Authors and Affiliations

  1. Institut für Zoologie, Universität Hohenheim, Tierökologie 220c, Garbenstraße 30, 70593, Stuttgart, Germany

    Christian König, Johannes L. M. Steidle & Till Tolasch

  2. Staatliches Museum für Naturkunde, Entomologische Abteilung, Rosenstein 1, 70191, Stuttgart, Germany

    Christian König

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  1. Christian König
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  2. Johannes L. M. Steidle
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  3. Till Tolasch
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Correspondence to Christian König.

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König, C., Steidle, J.L.M. & Tolasch, T. Decrypting Cryptic Click Beetle Species by Analysis of Sex Pheromones. J Chem Ecol 41, 740–745 (2015). https://doi.org/10.1007/s10886-015-0606-6

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  • DOI: https://doi.org/10.1007/s10886-015-0606-6

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